SEMA5B (BC002776) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SEMAG; SemG
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC002776 edited GGCACGAGGCTCCAGCATGGAGGAGGCCACCGGCTGTGCAGGGTTCAATCTCATCCACTT GGTGGCCACGGGCATCTCCTGCTTCTTGGGCTCTGGGCTCCTGACCCTAGCAGTGTACCT GTCTTGCCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCC CAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGA ATTCAAGACCCTGAACAAGAATAACTTGATCCCTGATGACAGAGCCAACTTCTACCCATT GCAGCAGACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTT CCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGG GGACTTGGGCTTCTTGCCTTCATAAGGCACAGAGCAGATGGAGATGGGACAGTGGAGCCA GTTTGGTTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCC ATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCT GACATATGGCCGCAGGTCCAGTTCAGCCCAGGTCTCTCATGGTTATCTTCCAACCCACTG TCACGCTGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTGAGGAATTG GAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCCTGTGG CCCCCACAAGCTGAGTCTGGCCTTCTCCAGCTGGCCCCAAAAAAGGCCTTTGCTACATCC TGATTATCTCTGAAAGTAATCAATCAAGTGGCTCCAGTAGCTCTGGATTTTCTGCCAGGG CTGGGCCATTGTGGTGCTGCCCCAGTATGACATGGGACCAAGGCCAGCGCAGGTTATCCA CCTCTGCCTGGAAGTCTATACTCTACCCAGGGCATCCCTCTGGTCAGAGGCAGTGAGTAC TGGGAACTGGAGGCTGACCTGTGCTTAGAAGTCCTTTAATCTGGGCTGGTACAGGCCTCA GCCTTGCCCTCAATGCACGAAAGGTGGCCCAGGAGAGAGGATCAATGCCATAGGAGGCAG AAGTCTGGCCTCTGTGCCTCTATGGAGACTATCTTCCAGTTGCTGCTCAACAGAGTTGTT GGCTGAGACCTGCTTGGGAGTCTCTGCTGGCCCTTCATCTGTTCAGGAACACACACACAC ACACACTCACACACGCACACACAATCACAATTTGCTACAGCAACAAAAAAGACATTGGGC TGTGGCATTATTAATTAAAGATGATATCCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002776 unedited NAAAATTCGGTCCGATCTTGTATAACACTCACTAAGGCGGCCGCGAAATNCGCACGAGGC CTCCAGCATGNAGGAGGCCAACCGGCTTGTGCAGGGTTCAATCTCATCCACTTGGTGGCC AACGGGCATCTCCTGCTTCTTGGGCTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTG CCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCCCAACCA TTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGAATTCAA GACCCTGAACAAGAATAACTTGATCCCTGATGACAGAGCCAACTTCTACCCATTGCAGCA GACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTTCCGGCC CGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTACTACCGCCGTCCTGGGGACTT GGGCTTCTTGCCTTCATAAGGCACAGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGG TTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCCATCCGG CTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCTGACATA TGGCCGCAGGTCCAGTTCAGCCCCAGTCTCTCATGGTTATCTTCCAACCCACTGTCACGC TGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTTGAGAATTGGAGAAT GGAGAATGGCAAGAGGGCAGGCTTTTTAGTTTTGGGTTGGAGACAACTTTTCTGTGGCCC CCCACAAGCTGAAGTCTGGCCTTCTCCAACTTGCCCCAAAAAAAGCCTTA
Restriction Sites	Please inquire
ACCN	BC002776
Insert Size	1383 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC002776.1, AAH02776.1
RefSeq Size	1383 bp
Locus ID	54437
Protein Families	Transmembrane
Protein Pathways	Axon guidance
Gene Summary	This gene encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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