TRAIL (TNFSF10) (BC032722) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Apo-2 ligand; Apo-2L; APO2L; CD253; TL2; TNF-related apoptosis inducing ligand TRAIL; TRAIL; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily; tumor necrosis factor apoptosis-inducing ligand splice variant delta
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC032722 edited CCACGCGTCCGAGAAGGAAGGGCTTCAGTGACCGGCTGCCTGGCTGACTTACAGCAGTCA GACTCTGACAGGATCATGGCTATGATGGAGGTCCAGGGGGGACCCAGCCTGGGACAGACC TGCGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTGTAACTTAC GTGTACTTTACCAACGAGCTGAAGCAGATGCAGGACAAGTACTCCAAAAGTGGCATTGCT TGTTTCTTAAAAGAAGATGACAGTTATTGGGACCCCAATGACGAAGAGAGTATGAACAGC CCCTGCTGGCAAGTCAAGTGGCAACTCCGTCAGCTCGTTAGAAAGATGATTTTGAGAACC TCTGAGGAAACCATTTCTACAGTTCAAGAAAAGCAACAAAATATTTCTCCCCTAGTGAGA GAAAGAGGTCCTCAGAGAGTAGCAGCTCACATAACTGGGACCAGAGGAAGAAGCAACACA TTGTCTTCTCCAAACTCCAAGAATGAAAAGGCTCTGGGCCGCAAAATAAACTCCTGGGAA TCATCAAGGAGTGGGCATTCATTCCTGAGCAACTTGCACTTGAGGAATGGTGAACTGGTC ATCCATGAAAAAGGGTTTTACTACATCTATTCCCAAACATACTTTCGATTTCAGGAGGAA ATAAAAGAAAACACAAAGAACGACAAACAAATGGTCCAATATATTTACAAATACACAAGT TATCCTGACCCTATATTGTTGATGAAAAGTGCTAGAAATAGTTGTTGGTCTAAAGATGCA GAATATGGACTCTATTCCATCTATCAAGGGGGAATATTTGAGCTTAAGGAAAATGACAGA ATTTTTGTTTCTGTAACAAATGAGCACTTGATAGACATGGACCATGAAGCCAGTTTTTTC GGGGCCTTTTTAGTTGGCTAACTGACCTGGAAAGAAAAAGCAATAACCTCAAAGTGACTA TTCAGTTTTCAGGATGATACACTATGAAGATGTTTCAAAAAATCTGACCAAAACAAACAA ACAGAAAACAGAAAACAAAAAAACCTCTATGCAATCTGAGTAGAGCAGCCACAACCAAAA AATTCTACAACACACACTGTTCTGAAAGTGACTCACTTATCCCAAGAAAATGAAATTGCT GAAAGATCTTTCAGGACTCTACCTCATATCAGTTTGCTAGCAGAAATCTAGAAGACTGTC AGCTTCCAAACATTAATGCAATGGTTAACATCTTCTGTCTTTATAATCTACTCCTTGTAA AGACTGTAGAAGAAAGCGCAACAATCCATCTCTCAAGTAGTGTATCACAGTAGTAGCCTC CAGGTTTCCTTAAGGGACAACATCCTTAAGTCAAAAGAGAGAAGAGGCACCACTAAAAGA TCGCAGTTTGCCTGGTGCAGTGGCTCACACCTGTAATCCCAACATTTTGGGAACCCAAGG TGGGTAGATCACGAGATCAAGAGATCAAGACCATAGTGACCAACATAGTGAAACCCCATC TCTACTGAAAGTGCAAAAATTAGCTGGGTGTGTTGGCACATGCCTGTAGTCCCAGCTACT TGAGAGGCTGAGGCAGGAGAATCGTTTGAACCCGGGAGGCAGAGGTTGCAGTGTGGTGAG ATCATGCCACTACACTCCAGCCTGGCGACAGAGCGAGACTTGGTTTCAAAAAAAAAAAAA AAAAAAACTTCAGTAAGTACGTGTTATTTTTTTCAATAAAATTCTATTACAGTAAAAAAA AAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032722 unedited GGGATCGGATTTGTATACGACTTACTATAGGCGGCCGCGTATTCAGATCTGGTACCGGTC CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGAGAAGGAAGGGCTTCAGTGACCGGC TGCCTGGCTGACTTACAGCAGTCAGACTCTGACAGGATCATGGCTATGATGGAGGTCCAG GGGGGACCCAGCCTGGGACAGACCTGCGTGCTGATCGTGATCTTCACAGTGCTCCTGCAG TCTCTCTGTGTGGCTGTAACTTACGTGTACTTTACCAACGAGCTGAAGCAGATGCAGGAC AAGTACTCCAAAAGTGGCATTGCTTGTTTCTTAAAAGAAGATGACAGTTATTGGGACCCC AATGACGAAGAGAGTATGAACAGCCCCTGCTGGCAAGTCAAGTGGCAACTCCGTCAGCTC GTTAGAAAGATGATTTTGAGAACCTCTGAGGAAACCATTTCTACAGTTCAAGAAAAGCAA CAAAATATTTCTCCCCTAGTGAGAGAAAGAGGTCCTCAGAGAGTAGCAGCTCACATAACT GGGACCAGAGGAAGAAGCAACACATTGTCTTCTCCAAACTCCAAGAATGAAAAGGCTCTG GGCCGCAAAATAAACTCCTGGGAATCATCAAGGAGTGGGCATTCATTCCTGAGCAACTTG CACTTGAGGAATGGTGAACTGGTCATCCATGAAAAAGGGTTTTACTACATCTATTCCCAA ACATACTTTCGATTTCAGGAGGAAATAACAGATAACACAGAGAACGACCAACAAATGGTC CAATATATTTACAAATACACCAGTTATTCCCTCGGGGGGACCCCCCGTTGNCGTGCAGTT CCCCCCTTTTTATTCCCATGTATTACGTGTTCCCCCTGTTTTTCCCCCATTTATCCAGAT GTGGGTGTGG
Restriction Sites	Please inquire
ACCN	BC032722
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC032722.1, AAH32722.1
RefSeq Size	1748 bp
RefSeq ORF	843 bp
Locus ID	8743
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity
Gene Summary	The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

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