DOPA Decarboxylase (DDC) (NM_001242886) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AADC
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330048 representing NM_001242886. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAACGCAAGTGAATTCCGAAGGAGAGGGAAGGAGATGGTGGATTACGTGGCCAACTACATGGAAGGC ATTGAGGGACGCCAGGTCTACCCTGACGTGGAGCCCGGGTACCTGCGGCCGCTGATCCCTGCCGCTGCC CCTCAGGAGCCAGACACGTTTGAGGACATCATCAACGACGTTGAGAAGATAATCATGCCTGGGGCGGCA AGCCCAGCATGCACAGAGCTGGAGACTGTGATGATGGACTGGCTCGGGAAGATGCTGGAACTACCAAAG GCATTTTTGAATGAGAAAGCTGGAGAAGGGGGAGGAGTGATCCAGGGAAGTGCCAGTGAAGCCACCCTG GTGGCCCTGCTGGCCGCTCGGACCAAAGTGATCCATCGGCTGCAGGCAGCGTCCCCAGAGCTCACACAG GCCGCTATCATGGAGAAGCTGGTGGCTTACTCATCCGATCAGGCACACTCCTCAGTGGAAAGAGCTGGG TTAATTGGTGGAGTGAAATTAAAAGCCATCCCCTCAGATGGCAACTTCGCCATGCGTGCGTCTGCCCTG CAGGAAGCCCTGGAGAGAGACAAAGCGGCTGGCCTGATTCCTTTCTTTATGGTTGCCACCCTGGGGACC ACAACATGCTGCTCCTTTGACAATCTCTTAGAAGTCGGTCCTATCTGCAACAAGGAAGACATATGGCTG CACGTTGATGCAGCCTACGCAGGCAGTGCATTCATCTGCCCTGAGTTCCGGCACCTTCTGAATGGAGTG GAGTTTGCAGATTCATTCAACTTTAATCCCCACAAATGGCTATTGGTGAATTTTGACTGTTCTGCCATG TGGGTGAAAAAGAGAACAGACTTAACGGGAGCCTTTAGACTGGACCCCACTTACCTGAAGCACAGCCAT CAGGATTCAGGGCTTATCACTGACTACCGGCATTGGCAGATACCACTGGGCAGAAGATTTCGCTCTTTG AAAATGTGGTTTGTATTTAGGATGTATGGAGTCAAAGGACTGCAGGCTTATATCCGCAAGCATGTCCAG CTGTCCCATGAGTTTGAGTCACTGGTGCGCCAGGATCCCCGCTTTGAAATCTGTGTGGAAGTCATTCTG GGGCTTGTCTGCTTTCGGCTAAAGGGTTCCAACAAAGTGAATGAAGCTCTTCTGCAAAGAATAAACAGT GCCAAAAAAATCCACTTGGTTCCATGTCACCTCAGGGACAAGTTTGTCCTGCGCTTTGCCATCTGTTCT CGCACGGTGGAATCTGCCCATGTGCAGCGGGCCTGGGAACACATCAAAGAGCTGGCGGCCGACGTGCTG CGAGCAGAGAGGGAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001242886
Insert Size	1329 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242886.1
RefSeq Size	1861 bp
RefSeq ORF	1329 bp
Locus ID	1644
UniProt ID	P20711
Protein Families	Druggable Genome
Protein Pathways	Histidine metabolism, Metabolic pathways, Phenylalanine metabolism, Tryptophan metabolism, Tyrosine metabolism
MW	49.7 kDa
Gene Summary	The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (3) lacks an in-frame coding exon, compared to variant 2. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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