RICTOR (NM_001285439) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AVO3; hAVO3; PIA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001285439, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGATCGGCCGCGGCCGCTCTCTGAAGAACCTCCGAGTACGAGGGCGGAATGACAGCGGCGAGG AGAACGTCCCGCTGGATCTGACCCGAGAACCTTCTGATAACTTAAGAGAGATTCTCCAAAATGTGGCCAG ATTGCAGGGAGTATCAAATATGAGAAAGCTAGGCCATCTGAATAACTTTACTAAGCTTCTTTGTGATATT GGCCACAGTGAAGAAAAACTGGGCTTTCACTATGAGGATATCATAATTTGTTTGCGGTTAGCTTTATTAA ATGAAGCAAAAGAAGTGCGAGCAGCAGGGCTACGAGCGCTTCGATATCTCATCCAAGACTCCAGTATTCT CCAGAAGGTGCTAAAATTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATACAACAGAGCAACGAG GTAGAGAGGACACAAGCACTTCGATTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTGTTTCCTAGTT CTGTGACCAACTCATTAATTGCAGTTGGAAATGATGGACTTCAAGAAAGAGACAGAATGGTCCGAGCATG CATTGCCATTATCTGTGAACTAGCACTTCAGAATCCAGAGGTGGTGGCCCTTCGAGGTGGACTAAACACC ATCTTGAAAAATGTGATCGATTGCCAATTAAGTCGAATAAATGAGGCCCTAATTACTACAATTTTGCACC TTCTTAATCATCCAAAGACTCGACAGTATGTGCGAGCTGATGTAGAATTAGAGAGAATTTTAGCACCCTA TACTGATTTTCACTACAGACATAGTCCAGATACAGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGA TTTCTAGCCAGTAAAATGGGAATCATAGCAACATTCCGATCATGGGCAGGTATTATTAATTTATGTAAAC CTGGAAATTCTGGGATCCAGTCTCTAATAGGAGTACTTTGCATACCAAATATGGAAATAAGGCGAGGTCT ACTTGAAGTGCTTTATGATATATTTCGTCTTCCTCTACCTGTTGTGACTGAGGAGTTCATAGAAGCACTA CTCAGTGTAGATCCAGGGAGGTTCCAAGACAGTTGGAGGCTTTCAGATGGCTTTGTGGCAGCTGAGGCAA AAACTATTCTTCCTCATCGTGCCAGATCCAGGCCAGACCTCATGGATAATTATTTGGCACTGATACTCTC TGCATTTATTCGTAATGGACTTTTAGAGGGTCTAGTTGAAGTGATAACAAACAGTGATGATCATATCTCA GTTAGAGCTACCATCCTTTTAGGAGAGCTTTTACATATGGCAAACACAATTCTTCCTCATTCACATAGCC ATCATTTACACTGCTTGCCAACCCTAATGAATATGGCTGCATCCTTTGATATCCCCAAGGAAAAGAGACT GCGAGCCAGTGCAGCCTTGAACTGTTTAAAACGCTTCCATGAAATGAAGAAACGAGGACCTAAGCCTTAT AGTCTTCATTTAGACCACATTATTCAGAAAGCAATTGCAACACACCAGAAACGGGATCAGTATCTCCGAG TTCAGAAAGATATATTTATCCTTAAGGATACAGAGGAAGCTCTTTTAATTAACCTTAGAGATAGCCAAGT CCTTCAACATAAAGAGAATCTTGAATGGAATTGGAATCTTATAGGGACCATTCTTAAGTGGCCAAATGTA AATCTAAGAAACTATAAAGATGAACAGTTACACAGGTTTGTACGAAGACTACTTTATTTTTACAAGCCCA GCAGTAAATTATATGCCAACCTGGATCTGGATTTTGCCAAGGCCAAACAGCTCACGGTTGTAGGTTGCCA GTTTACAGAATTTCTTCTTGAATCTGAAGAGGATGGGCAAGGCTACTTAGAAGATCTAGTAAAGGATATT GTTCAGTGGCTCAATGCTTCATCTGGAATGAAACCCGAAAGAAGTCTTCAAAATAATGGTTTATTGACCA CCCTTAGTCAACACTACTTTTTATTTATTGGAACACTTTCTTGCCACCCTCATGGAGTTAAAATGCTGGA AAAATGCAGTGTATTTCAGTGTCTCCTTAATCTTTGCTCCTTGAAAAACCAAGATCACTTGCTAAAACTT ACTGTTTCTAGCTTGGACTATAGCAGAGATGGATTGGCTAGAGTCATCCTTTCCAAAATTTTAACTGCAG CTACTGATGCCTGCAGACTCTATGCAACAAAACATTTAAGGGTATTATTGAGAGCTAATGTTGAATTCTT TAATAATTGGGGAATTGAGTTGTTAGTGACCCAGCTACATGATAAAAACAAAACGATTTCCTCTGAAGCT CTTGATATCCTCGATGAAGCATGTGAAGACAAGGCCAATCTTCATGCTCTCATTCAGATGAAACCAGCGT TATCCCACCTTGGAGACAAGGGTTTGCTTCTCCTGCTGAGATTTCTCTCCATTCCAAAAGGATTTTCCTA TCTGAATGAAAGAGGTTATGTAGCAAAACAATTGGAAAAGTGGCACAGGGAATACAACTCCAAATATGTT GACTTGATTGAGGAACAACTCAATGAAGCACTTACTACTTACCGGAAGCCTGTTGATGGTGATAACTATG TTCGTCGGAGTAACCAAAGATTACAGCGTCCTCACGTCTACCTGCCTATACACCTTTATGGACAACTAGT ACACCATAAAACAGGCTGCCATTTGTTGGAAGTACAGAATATTATTACAGAACTCTGTCGTAATGTTCGT ACACCAGATTTGGATAAGTGGGAAGAAATTAAAAAACTGAAAGCATCTCTTTGGGCCTTGGGAAATATCG GCTCATCAAATTGGGGTCTCAATTTGCTACAGGAAGAAAACGTGATTCCAGATATACTAAAACTTGCAAA ACAGTGTGAAGTTCTTTCCATCAGAGGGACCTGTGTATATGTACTTGGGCTCATAGCTAAAACCAAACAA GGCTGTGATATTCTAAAATGTCACAACTGGGATGCTGTGAGGCATAGTCGCAAACATCTGTGGCCAGTGG TTCCAGATGATGTGGAACAACTCTGTAATGAACTTTCATCTATCCCAAGCACTCTAAGTTTGAACTCGGA GTCAACCAGCTCTAGACATAATAGTGAAAGTGAATCTGTGCCATCGAGTATGTTCATATTGGAGGATGAC CGGTTTGGCAGCAGCTCTACTAGTACATTTTTCCTTGATATCAATGAAGATACAGAGCCAACATTTTATG ACCGATCTGGACCCATAAAGGATAAAAATTCATTCCCTTTCTTTGCTTCTAGTAAACTTGTGAAGAATCG TATCTTAAATTCGCTTACTTTGCCTAACAAAAAACATCGTAGTAGCAGTGATCCAAAAGGAGGGAAATTA TCATCTGAAAGTAAGACAAGCAACAGGCGAATCAGAACACTTACGGAGCCCAGTGTTGATTTTAATCATA GTGATGATTTTACACCCATATCCACTGTACAGAAAACATTACAATTAGAGACTTCATTTATGGGGAATAA GCACATTGAAGACACTGGTAGTACACCAAGCATTGGAGAAAATGACTTAAAATTCACCAAGAATTTTGGT ACAGAGAATCACAGAGAAAATACAAGCCGAGAGAGGTTAGTAGTAGAAAGTTCAACGAGCTCACATATGA AGATACGTAGCCAAAGTTTCAATACAGACACTACAACAAGTGGCATAAGTTCAATGAGCTCAAGTCCTTC ACGAGAGACAGTAGGTGTAGATGCTACAACTATGGACACAGACTGTGGAAGCATGAGTACTGTGGTAAGT ACTAAAACTATTAAGACAAGCCACTATTTGACGCCACAGTCTAACCATCTGTCTCTCTCCAAATCAAATT CGGTGTCCCTGGTGCCTCCAGGTTCTTCTCATACGCTTCCTAGAAGAGCACAGTCCCTTAAAGCACCCTC TATTGCTACAATTAAAAGTCTAGCAGATTGTAACTTTAGTTACACAAGTTCTAGAGATGCTTTTGGCTAT GCTACACTGAAAAGACTACAGCAACAAAGAATGCATCCATCCTTATCTCACTCTGAAGCTTTGGCATCTC CAGCAAAAGATGTGCTATTTACTGATACCATCACCATGAAGGCCAACAGTTTTGAGTCCAGATTAACACC AAGCAGGATCGATTTTAAAAAGAAGCATGTCGGGGGAATCAGGAGCTTAAGACCTACAATAACAAACAAC CTTTTCAGGTTCATGAAAGCCTTAAGTTATGCATCATTAGATAAAGAAGATTTATTGAGTCCTATTAATC AAAATACCCTGCAACGATCTTCCTCAGTGCGGTCCATGGTGTCCAGTGCCACATATGGGGGTTCAGATGA TTACATTGGTCTTGCTCTCCCGGTGGATATAAATGATATATTCCAGGTAAAGGATATTCCCTATTTTCAG ACAAAAAACATACCACCACATGATGATCGAGGTGCAAGAGCATTTGCCCATGATGCAGGAGGTCTTCCAT CTGGAACTGGAGGTCTTGTAAAAAATTCTTTTCACTTGCTACGACAGCAGATGAGTCTTACGGAAATAAT GAATTCAATCCATTCAGATGCCTCTCTGTTTTTAGAAAGTACAGAAGACACTGGACTACAGGAACATACA GATGATAACTGCCTTTATTGTGTCTGTATTGAAATTCTGGGTTTCCAGCCCAGCAACCAACTGAGTGCAA TATGTAGTCATTCAGACTTTCAAGATATTCCATATTCTGATTGGTGTGAGCAGACTATCCATAATCCTTT AGAAGTGGTTCCCTCTAAGTTTTCGGGGATTTCTGGATGCAGTGATGGGGTGTCTCAAGAAGGCTCAGCT AGCAGCACCAAAAGCACAGAATTGTTACTAGGTGTTAAAACAATTCCAGATGATACACCAATGTGCCGTA TACTCCTTCGCAAAGAAGTTCTAAGATTAGTCATTAATTTGAGTAGTTCAGTTTCAACTAAATGTCATGA GACTGGGCTTTTAACAATTAAGGAGAAGTATCCTCAAACATTTGATGACATATGCCTTTACTCTGAGGTT TCCCATTTGCTGTCACACTGCACATTCAGACTTCCGTGTCGGAGGTTCATACAAGAATTATTTCAAGATG TACAGTTTCTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAACACCACCAAAGCAACCTATAGTTGA TACATCTGCTGAATCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001285439
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285439.1, NP_001272368.1
RefSeq Size	9629 bp
RefSeq ORF	5199 bp
Locus ID	253260
UniProt ID	Q6R327
Protein Pathways	mTOR signaling pathway
Gene Summary	RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1. The resulting longer isoform (2) has an internal protein segment not found in isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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