Proteins
Calmodulin bovine protein, 1 mg
Alpha-2-HS-glycoprotein / AHSG bovine protein, 1 g
| Protein Source | Serum |
RNASE1 bovine protein, 0.1 g
Thyroglobulin bovine protein, 0.1 g
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- 2 mM dithiothreitol (8)
- the solution can be further dialyzed against PBS (phosphate buffered saline (8)
- 10 mM Tris-HCl (6)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization. (4) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (4)
-
e.g. Dulbeccos PBS).
See References 2 and 3 for more details. (4) -
pH 7.4).
For Immunization purposes (4) - ELISA. (3)
- 10 mM sodium phosphate (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
- e.g. Dulbeccos PBS). (2)
- pH 7.4). For immunization purposes (2)
-
pH 7.4).
For immunization purposes (2) - protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
-
ELISA.
Functional Assays. (1)
