Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

Mutant ORF Clones

CytoSections

Mass Spec Standards

Mutant Proteins

Over-expression Lysates

Human

P53

MSH2

NM23A

PCNA

BCL2

CD30

MSH6

TGIF

CD117

ERBB 3

BNP

APOLIPOPROTEIN E

CHK2

BUBR1

PPM1B

AURORA A

PAXILLIN

HMGA1

RBPMS

HSP60

PROTEASOME SUBUNIT ALPHA TYPE 6

UNG

BIRC5

CARDIAC TROPONIN I

APE1

C-MYC

MTH1

RFC2

RPA34

MCM2

EWSR1

PRMT5

MYH

OCT4

HDAC1

SSB

ANNEXIN A2

MAP126

C-MYB

BAT3

CTBP2

VASP

CASPASE 9

GEMIN 2

CHK1

EXOSC7

ANNEXIN A3

NUCLEOPHOSMIN

SIRT1

CDK1

NUCLEOSTEMIN

OTX2

SHP1

CYCLIN D1

DNMT3B

HSP90AB1

HNRNP Q

PBX1

PRION PROTEIN PRP

KAP1

PASK

RAD51

SUMO 1

GRB7

LSM1

RFC3

DAXX

PAK3

SNRPN

SYNTAXIN 3

FEN1

SPT3

KPNB1

ATIC

HMG1

TRBP

TRIP

CD168

GRP75

LCK

PIAS2

EXOSC3

FBP1

LAP2

IKB BETA

PIRH2

RAD9

DHFR

MATK

MRE11

PHGDH

REX1

SMN1

WDR77

CDC25C

CDC45L

RFC4

CASPASE-6

PAK1

SMARCAD1

Human

Mouse

Rat

Monkey

Dog

Bovine

Human (Expected from sequence similarity: Mouse

Chicken

Pig

Cow)

Human (Predicted: Mouse

Hamster

Canine

Rabbit

Dog)

Xenopus

Bovine)

Primate

Sheep

Drosophila

Monkey)

Chimpanzee

Mouse (Predicted: Rat

Rhesus Monkey)

Guinea Pig

Human (Predicted: Mouse)

Pig)

X. tropicalis)

Yeast

Hamster)

Human (Predicted: Rat

Mouse (Expected from sequence similarity: Rat

Zebrafish

Chicken)

Cow

Human (Predicted: Bovine

Rabbit)

Rat)

Xenopus)

Equine

Feline

Fish

Frog

Gerbil

Goat

Human (Expected from sequence similarity: Dog

Human (Predicted: Bovine)

Human (Predicted: Chicken

Human (Predicted: Monkey)

Mammalian

Mouse)

Porcine

Rat (Expected from sequence similarity: Dog

Rat (Expected from sequence similarity: Dog)

Rhesus Monkey

Saccharomyces cerevisiae

Algal

Avian

Baboon

Beluga

C. elegans

Canis

Escherichia coli

Fruit fly

Fungi

Helicobacter pylori

Horse

Human (Expected from sequence similarity: Cow)

Human (Expected from sequence similarity: Dog)

Human (Expected from sequence similarity: Feline

Human (Expected from sequence similarity: Mouse)

Human (Expected from sequence similarity: Pig

Human (Expected from sequence similarity: Pig)

Human (Expected from sequence similarity: Rat

Human (Predicted: Chimpanzee

Human (Predicted: Cow)

Human (Predicted: Dog

Human (Predicted: Hamster

Human (Predicted: Monkey

Human (Predicted: Pig

Human (Predicted: Rat)

Human (Predicted: Rhesus Monkey)

Insect

Mouse (Does not react with: Rat)

Mouse (Expected from sequence similarity: Human

Mouse (Predicted: Bovine)

Mouse (Predicted: Chimpanzee

Mouse (Predicted: Monkey)

Mouse (Predicted: Pig

Mouse (Predicted: Pig)

Pig (Expected from sequence similarity: Dog

Pig (Expected from sequence similarity: Dog)

Pig (Expected from sequence similarity: Feline

Plant

Rat (Predicted: Bovine

Rat (Predicted: Mouse

Salmonella Typhimurium

Spinach

Trichinella spiralis

White Fly

X. tropicalis

Yeast (Does not react with: Xenopus)

WB

IHC

IF

FC

ELISA

IP

ICC/IF

LMNX

10k-ChIP

Simple Western

ChIP

Dot

PEP-ELISA

Immunoblotting

CyTOF-ready

Block/Neutralize

ICC

SISCAPA

Electron Microscopy

FN

ID

Sandwich ELISA

Mouse

Rabbit

Goat

Chicken

Human

Rat

HEK293T

HEK293

E. coli

Sf9

Plasmids

Myc-DDK

C-Myc/DDK

Tag Free

mGFP

TurboGFP

C-MYC/DDK

N-His

C-His

C-DDK

N-GST and C-His

C-DDK/His

DDK-His

N-His&C-His

N-MARI

C-Fc

N-GST and C-HIS

C-6His

C-6His-Avi

C-mFc

His-tag

C-6×His

C-HIS

N-DDK

N-His&Flag

N-His-ABP (Albumin-Binding Protein)

N-His-GST

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-AC

pCMV6-AC-Myc-DDK

pCMV6-XL4

pCMV6-AN-Myc-DDK

pCMV6-XL6

PCMV6-Neo

pCMV6-XL5-DDK-His

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

DyLight 488

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG3

IgG2a, kappa

IgG2c

IgG2b, kappa

IgM, kappa

IgG3, kappa

IgM

IgY

Monoclonal

Polyclonal

No

Yes

Indirect

Sandwich

Direct

TrueMAB

LentiORF

TrueORF

TrueClone

MVPro

CytoSections

TrueORF Gold, TrueORF

UltraMAB

TrueRAB

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

WB

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Nuclear Hormone Receptor

Embryonic stem cells

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Pathways in cancer

Cell cycle

Colorectal cancer

p53 signaling pathway

Mismatch repair

Small cell lung cancer

MAPK signaling pathway

Prostate cancer

Chronic myeloid leukemia

Metabolic pathways

Amyotrophic lateral sclerosis (ALS)

Apoptosis

DNA replication

Wnt signaling pathway

Huntington's disease

Endometrial cancer

Neurotrophin signaling pathway

Pancreatic cancer

Bladder cancer

Thyroid cancer

Non-small cell lung cancer

Nucleotide excision repair

Base excision repair

Glioma

Melanoma

Basal cell carcinoma

Focal adhesion

Purine metabolism

Pyrimidine metabolism

RNA degradation

Spliceosome

Homologous recombination

Endocytosis

ErbB signaling pathway

Oocyte meiosis

Jak-STAT signaling pathway

Acute myeloid leukemia

Cytokine-cytokine receptor interaction

Regulation of actin cytoskeleton

Progesterone-mediated oocyte maturation

T cell receptor signaling pathway

Alzheimer's disease

Chemokine signaling pathway

Proteasome

Notch signaling pathway

Leukocyte transendothelial migration

VEGF signaling pathway

Viral myocarditis

Calcium signaling pathway

Natural killer cell mediated cytotoxicity

Antigen processing and presentation

Primary immunodeficiency

Hematopoietic cell lineage

Melanogenesis

Ubiquitin mediated proteolysis

Fc gamma R-mediated phagocytosis

Systemic lupus erythematosus

TGF-beta signaling pathway

Axon guidance

Non-homologous end-joining

One carbon pool by folate

B cell receptor signaling pathway

NOD-like receptor signaling pathway

Cytosolic DNA-sensing pathway

Cardiac muscle contraction

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

Type I diabetes mellitus

Drug metabolism - other enzymes

Gap junction

Renal cell carcinoma

Prion diseases

Parkinson's disease

Cysteine and methionine metabolism

Insulin signaling pathway

SNARE interactions in vesicular transport

Adherens junction

RNA polymerase

Glycerophospholipid metabolism

ECM-receptor interaction

Fructose and mannose metabolism

Glycolysis / Gluconeogenesis

Pentose phosphate pathway

Adipocytokine signaling pathway

RIG-I-like receptor signaling pathway

Folate biosynthesis

Glycine

Pathogenic Escherichia coli infection

serine and threonine metabolism

Epithelial cell signaling in Helicobacter pylori infection

Type II diabetes mellitus

Selenoamino acid metabolism

Ether lipid metabolism

Glycerolipid metabolism

Sphingolipid metabolism

Alanine

Arginine and proline metabolism

Phenylalanine

Phenylalanine metabolism

Tyrosine metabolism

aspartate and glutamate metabolism

tyrosine and tryptophan biosynthesis

Steroid biosynthesis

Glyoxylate and dicarboxylate metabolism

Basal transcription factors

Nicotinate and nicotinamide metabolism

Human

E. coli

Human 293

96-well plate

96-well plate, Luminex

Luminex

Neomycin

Puromycin

None

Recombinant Antibodies

TA600521

TA600538

TA700515

TA700523

TA600006

TA600032

TA600033

TA600053

TA600459

TA600459, TA600460, TA600463

TA600460

TA600461

TA600462

TA600496

TA600497

TA600515, TA600516

TA600523, TA600524

TA600539

TA600540

TA600562

TA700006

TA700032

TA700033

TA700459

TA700459, TA700460

TA700459, TA700461

TA700462

TA700463

TA700496

TA700497

TA700521, TA700531

TA700522

TA700538, TA700541

TA700539

TA700540

TA700562

Carrier-free Antibodies

1

2

3

Yes

In Stock