ADO (Myc-DDK-tagged)-Human 2-aminoethanethiol (cysteamine) dioxygenase (ADO)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Recombinant protein of human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), 20 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Ado (Myc-DDK-tagged) - Mouse 2-aminoethanethiol (cysteamine) dioxygenase (Ado)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Ado (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Ado - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Ado (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
ADO - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Ado - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Recombinant protein of human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of 2-aminoethanethiol (cysteamine) dioxygenase (ADO)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
ADO - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control |
| Donor DNA | GFP-puro |
ADO CRISPRa kit - CRISPR gene activation of human 2-aminoethanethiol dioxygenase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
ADO - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control |
| Donor DNA | mBFP-Neo |
ADO - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
