GCLM (Myc-DDK-tagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Gclm (Myc-DDK-tagged) - Mouse glutamate-cysteine ligase, modifier subunit (Gclm)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
GCLM - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
GCLM - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Gclm - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Recombinant protein of human glutamate-cysteine ligase, modifier subunit (GCLM), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human glutamate-cysteine ligase, modifier subunit (GCLM), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of glutamate-cysteine ligase, modifier subunit (GCLM)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
GCLM HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
GCLM CRISPRa kit - CRISPR gene activation of human glutamate-cysteine ligase modifier subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Gclm CRISPRa kit - CRISPR gene activation of mouse glutamate-cysteine ligase, modifier subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Mus musculus gene Gclm
qPCR primer pairs and template standards against Homo sapiens gene GCLM
| Application | Plasmid of exact quantity for transcript copy number calculation |
Purified recombinant protein of Mouse glutamate-cysteine ligase, modifier subunit (Gclm), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
Gclm (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
