HSD11B2 (Myc-DDK-tagged)-Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Hsd11b2 (Myc-DDK-tagged) - Mouse hydroxysteroid 11-beta dehydrogenase 2 (Hsd11b2)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
HSD11B2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Hsd11b2 - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
Hsd11b2 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
HSD11B2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
HSD11B2 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Hsd11b2 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Recombinant protein of human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2), 1 mg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Recombinant protein of human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2), 100 µg
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
HSD11B2 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
HSD11B2 CRISPRa kit - CRISPR gene activation of human hydroxysteroid 11-beta dehydrogenase 2
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Hsd11b2 CRISPRa kit - CRISPR gene activation of mouse hydroxysteroid 11-beta dehydrogenase 2
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene HSD11B2
| Application | Plasmid of exact quantity for transcript copy number calculation |
