6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: 16-001]

Specifications

Product Data
Clone Name	16-001
Applications	WB
Recommend Dilution	ELISA. Western blot.
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody rects with recombinant proteins-(His)6.
Isotype	IgG1
Formulation	PBS pH7.4, with 15mM sodium azide State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20 to -70 °C. Avoid repeated freezing and thawing.
Background	6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Synonyms	6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag
Reference Data

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