CRISPR / Cas9，基因编辑工具
什么是CRISPR？
CRISPR / Cas9是一种RNA导向的靶向基因组编辑工具，可让研究人员针对细胞系或动物进行基因敲除、SNP、插入和缺失。 CRISPR / Cas9基因组编辑系统需要两个组件，Cas9，核酸内切酶和向导RNA（sgRNA）；sgRNA特异性地将Cas9引导至基因组序列中的位置。 在3'末端存在与原间隔物相邻的基序（PAM-序列NGG），Cas9将解开DNA双链体并在被引导RNA识别靶序列后切割两条链。
产品与服务
OriGene提供的试剂支持广泛的CRISPR / Cas9基因编辑应用，单击下图与基因编辑相关的产品，详细了解我们可提供的服务：
Gene Knockout Kits
CRISPR/CAS9 Vectors
gRNA and donor cloning
AAVS1/ROSA26 Transgene Insertion
Functional CAS9 Protein
CRISPRa CRISPRi
CRISPR资源
- Learn CRISPR from our Youtube Channel
- Webinar from Cell: Expanding CRISPR Tool Box
- Frequently Asked Questions
- HA tagging Validation Data
- CRISPR Knockout kit Manual
- CRISPR vector Manual
- CRISPR Trangene Knockin at AAVS1 and ROSA26 loci Manual
- CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) Manual
- CRISPR eBook
References
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Multiplex
Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD,
Wu X, Jiang W, Marraffini LA, Zhang F.
Science 2013 Feb 15;339(6121):819-23 -
RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM
Science 2013 Feb 15;339(6121):823-6
近期文献应用
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1. GBA Regulates EMT/MET and Chemoresistance in Squamous Cell Carcinoma Cells by Modulating the Cellular Glycosphingolipid Profile Published in Cells , 2023 Jul , using KN201614BN |
PubMed: 37508550 |
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2. Regulation of microRNA expression by the adaptor protein GRB2 Published in Scientific Reports, 2023 Jun , using KN200469 |
PubMed: 37328606 |
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3. Development of a Novel σ 1 Receptor Biosensor Based on Its Heterodimerization with Binding Immunoglobulin Protein in Living Cells. Published in ACS chemical neuroscience, 2023 Jun , using KN201206 |
PubMed: 37191585 |
