Itgax Rat Monoclonal Antibody [Clone ID: 223H7]

Specifications

Product Data
Clone Name	223H7
Applications	FC
Recommend Dilution	Flow Cytometry: 10 μg/ml (final concentration). For details see Protocol below.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Murine dendritic cells isolated from C57BL/6 mice
Specificity	This antibody reacts with Mouse CD11c antigen on Flow Cytometry. Other species not tested.
Isotype	IgG2a
Formulation	PBS Label: FITC State: Liquid purified Ig fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Protein G Agarose Chromatography
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	integrin alpha X
Database Link	Entrez Gene 16411 Mouse UniProt ID Q9QXH4
Background	The CD11c ( α X integrin; ~150 kDa) glycoprotein non-covalently associates with CD18 ( β 2 integrin; ~95 kDa) to form the heterodimeric complement receptor type 4 (CR4), which is involved in monocyte/granulocyte adhesion during inflammatory responses. The CD11c/CD18 receptor binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. CD11c/CD18 is also implicated in B cell proliferation and mediates B cell binding to fibrinogen. CD11c is commonly used as a marker for dendritic cells, but it is also expressed on macrophages, monocytes, granulocytes, NK cells, activated T and B lymphocytes and microglia.
Synonyms	ITGAX, Integrin alpha-X, Leu M5
Note	This product was originally produced by MBL International. Protocol: Flow Cytometric analysis for floating cells We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3]. 2) Resuspend the cells with washing buffer (5x106 cells/mL). 3) Add 50 μL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 20 μL of normal goat serum containing 1 mg/mL normal human IgG and 0.1% NaN3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 40 μL of the FITC labeled mouse CD11c monoclonal antibody (223H7) (10 μ g/mL) diluted in the washing buffer. Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Add 30 μL of 1:50 Biotin labeled anti-mouse I-Ab (Aaβb) diluted with the washing buffer. Mix well and incubate for 15 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 9) Add 30 μL of 1:50 PE labeled streptavidin diluted with the washing buffer. Mix well and incubate for 15 minutes at room temperature. 10) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 11) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. (Positive control for Flow cytometry; mouse splenocyte)
Reference Data

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