CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: UB2]

CAT＃: AM26668FC-N

CD95 (FAS) mouse monoclonal antibody, clone UB2, FITC

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CNY 5764.00

货期*
5周
规格
    • 50 Tests
Cited in 13 publications.

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Specifications

Product Data
Clone Name UB2
Applications FC
Recommend Dilution Flow cytometry: 20 μl (ready for use).
For details see protocol below.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Recombinant human Fas
Specificity This antibody recognizes the human Fas antigen specifically. Clone UB2 does not recognize the mouse Fas antigen.
Isotype IgG1
Formulation BSA
Label: FITC
State: Liquid Ig fraction
Stabilizer: 1% BSA
Preservative:
0.09% NaN3
Purification Protein A agarose
Conjugation FITC
Storage Condition Store at 2-8 °C.
Gene Name Fas cell surface death receptor
Database Link
Background It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology with a number of cell-surface receptors, includin g tumor necrosis factor receptor and nerve growth factor receptor.
Synonyms FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Note This product was originally produced by MBL International.

Protocol: Flow cytometric analysis for floating cells
We usually use Fisher tubes or equivalents as reaction tubes for all steps described below.
1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN 3].
2) Resuspend the cells with washing buffer (5 x 10 6 cells/mL).
3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration.
4) Add 20 μ L of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
5) Add 20 μ L of FITC labeled anti-Fas monoclonal antibody (UB2). Mix well and incubate for 30 minutes at room temperature.
6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer.
(Positive control for Flow cytometry; transfectant)
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (13)

The use of this Antibodies has been cited in the following citations:
Fas drives cell cycle progression in glioma cells via extracellular signal-regulated kinase activation ,Shinohara H, Yagita H, Ikawa Y, Oyaizu N, Cancer Res. ,PubMed ID 10749152 [FAS]
Suppression of Akt signaling induces Fas ligand expression: involvement of caspase and Jun kinase activation in Akt-mediated Fas ligand regulation ,Suhara T, Kim HS, Kirshenbaum LA, Walsh K, Mol. Cell. Biol. ,PubMed ID 11756562 [FAS]
Induction and regulation of Fas-mediated apoptosis in human thyroid epithelial cells ,Mezosi E, Wang SH, Utsugi S, Bajnok L, Bretz JD, Gauger PG, Thompson NW, Baker JR, Mol. Endocrinol. ,PubMed ID 15563545 [FAS]
Expression of Fas/Apo-1 (CD95) and apoptosis in tumor cells from patients with plasma cell disorders ,Hata H, Matsuzaki H, Takeya M, Yoshida M, Sonoki T, Nagasaki A, Kuribayashi N, Kawano F, Takatsuki K, Blood ,PubMed ID 7544648 [FAS]
Perforin, Fas/Fas ligand, and TNF-alpha pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL ,Ando K, Hiroishi K, Kaneko T, Moriyama T, Muto Y, Kayagaki N, Yagita H, Okumura K, Imawari M, J. Immunol. ,PubMed ID 9164947 [FAS]
Homocysteine enhances endothelial apoptosis via upregulation of Fas-mediated pathways ,Suhara T, Fukuo K, Yasuda O, Tsubakimoto M, Takemura Y, Kawamoto H, Yokoi T, Mogi M, Kaimoto T, Ogihara T, Hypertension ,PubMed ID 15117910 [FAS]
Fas ligand is present in human erythroid colony-forming cells and interacts with Fas induced by interferon gamma to produce erythroid cell apoptosis ,Dai CH, Price JO, Brunner T, Krantz SB, Blood ,PubMed ID 9454753 [FAS]
The involvement of Fas-Fas ligand pathway in fibrosing lung diseases ,Kuwano K, Miyazaki H, Hagimoto N, Kawasaki M, Fujita M, Kunitake R, Kaneko Y, Hara N, Am. J. Respir. Cell Mol. Biol. ,PubMed ID 9870917 [FAS]
Functional Fas ligand expression in thyrocytes from patients with Graves' disease ,Hiromatsu Y, Hoshino T, Yagita H, Koga M, Sakisaka S, Honda J, Yang D, Kayagaki N, Okumura K, Nonaka K, J. Clin. Endocrinol. Metab. ,PubMed ID 10443697 [FAS]
Rituximab-induced inhibition of YY1 and Bcl-xL expression in Ramos non-Hodgkin's lymphoma cell line via inhibition of NF-kappa B activity: role of YY1 and Bcl-xL in Fas resistance and chemoresistance, respectively ,Vega MI, Jazirehi AR, Huerta-Yepez S, Bonavida B, J. Immunol. ,PubMed ID 16081784 [FAS]
Functional proteomic screen identifies a modulating role for CD44 in death receptor-mediated apoptosis ,Hauptschein RS, Sloan KE, Torella C, Moezzifard R, Giel-Moloney M, Zehetmeier C, Unger C, Ilag LL, Jay DG, Cancer Res. ,PubMed ID 15753387 [FAS]
Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in sensitivity to Fas ligand-induced cell death: implications for vascular disease and therapy ,Sata M, Suhara T, Walsh K, Arterioscler. Thromb. Vasc. Biol. ,PubMed ID 10669625 [FAS]
Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis ,Boirivant M, Pica R, DeMaria R, Testi R, Pallone F, Strober W, J. Clin. Invest. ,PubMed ID 8958226 [FAS]

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