Melanoma (25-110 kDa) Mouse Monoclonal Antibody [Clone ID: NKI/C3]

Specifications

Product Data
Clone Name	NKI/C3
Applications	IHC
Recommend Dilution	Immunohistochemistry on Paraffin Sections. Standard ABC technique. Working Dilution: 1/10-1/20. Incubation Time: 60 minutes at 25°C. Positive Control: Tonsil. Staining Pattern: Cytoplasmic and membrane.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Smooth plasma membrane fraction of MeWo cells.
Specificity	This antibody clone NKI/C-3 recognises a heterogeneous 25-110 kD glycoprotein that is located mainly in the innerside of membranes of cytoplasmic vesicles in melanoma cells. The antigen has a 25 kD unglycosylated precursor in formalin fixed and paraffin-embedded tissue sections. Cross-reactivity: The antibody reacts with some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions and with cells with secretory functions such as salivary glands, bronchial glands, sweat glands, pancreas and prostate.
Isotype	IgG1
Formulation	50mM PBS, pH 7.4 State: Ascites State: Liquid diluted Ascites Stabilizer: 1% BSA Preservative: 15mM Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	Melanoma marker
Reference Data

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