Hepatocyte Specific Antigen (Hepatocellular Marker) Mouse Monoclonal Antibody [Clone ID: HSA133]

Specifications

Product Data
Clone Name	HSA133
Applications	IF, IHC
Recommend Dilution	Immunofluorescence: 0.5-1 µg/ml. Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 µg/ml for 30 minutes. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. Recommended Positive Control: Normal liver or hepatocellular carcinoma (HCC).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	SK-H1A9-2 human hepatocellular carcinoma cells
Specificity	Monoclonal Antibody HSA133 stains Human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellul ar carcinoma biopsies and cell lines are found to bind this MAb on the cell surface. HSA133 str ongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraf ormaldehyde fixed cell preparations. Cellular Localization: Cell Surface.
Isotype	IgG2b
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Background	Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
Reference Data

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