BCL2 Mouse Monoclonal Antibody [Clone ID: 100/D5+124]

Specifications

Product Data
Clone Name	100/D5+124
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1ug/million cells. Immunofluorescence: 0.5-1 µg/ml. Western Blotting: 0.5-1 µg/ml. Immunoprecipitation: 5-1 µg/500 µg protein lysate. Immunohistochenmsitry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide, amino acids 41-54 (GAAPAPGIFSSQPG-Cys) of Human Bcl-2 (100/D5 & 124)
Specificity	This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative. Cellular Localization: Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. Negative Species: Mouse and Rat.
Isotype	IgG1
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	25-26 kDa
Gene Name	B-cell CLL/lymphoma 2
Database Link	Entrez Gene 596 Human UniProt ID P10415
Synonyms	BCL2, Bcl-2 alpha
Reference Data

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