WNT3A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Sandwich ELISA: To detect Human WNT-3a by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml is required. This affinity purified polyclonal antibody, in conjunction with Biotin conjugated Polyclonal Anti-Human WNT-3a (AP00591BT-N or AP00591BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a. Western Blot: To detect Human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 0.125-0.25 µg/ml with an overnight incubation at 4°C. This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human breast invasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommneded. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	E.Coli derived recombinant Human WNT-3a
Specificity	This antibody detects Human WNT-3a. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Wnt family member 3A
Database Link	Entrez Gene 89780 Human UniProt ID P56704
Synonyms	Protein Wnt-3a
Note	Centrifuge vial prior to opening.
Reference Data

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