Galectin 3 (LGALS3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA Indirect: To detect hGalectin-3 (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. In conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-3. Sandwich: To detect Human Galectin-3 (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. In conjunction with Biotinylated Polyclonal Anti-Human Galectin-3 (AP01146BT-N or AP01146BT-S) as a Detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-3. Western blot To detect Human Galectin-3, this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Galectin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry. 31.25 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection systemwas used with a DAB Chromogen. Heat induced antigen retrieval with pH 6.0 Sodium Citrate buffer. This antibody stained Formalin-Fixed paraffin-Embedded Sexctions of Human Pancreas infiltrating ductal Adenocarcinoma tissue.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) recombinant Human Galectin-3
Specificity	This antibody detects Galectin-3.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	lectin, galactoside binding soluble 3
Database Link	Entrez Gene 3958 Human UniProt ID P17931
Background	Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.
Synonyms	Mac-2, Lgals3, GAL3, GALBP, CBP35, L-31
Note	Centrifuge vial prior to opening!
Reference Data

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