Oct4 (POU5F1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 200-250 of Human Oct-3/4.
Specificity	The antibody detects endogenous levels of OCT3/4 protein. (region surrounding Lys222)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen. The purity is > 95% (by SDS-PAGE).
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 38 kDa
Gene Name	POU class 5 homeobox 1
Database Link	Entrez Gene 18999 MouseEntrez Gene 294562 RatEntrez Gene 5460 Human UniProt ID Q01860
Background	Organic cation transporters (OCT) are expressed in the plasma membrane of epithelial cells from a wide range of tissues, where they function in the elimination of ndogenous amines, cationic drugs and other xenobiotics. The structure of OCTs consists of a 12-transmembrane-domain structure and a large extracellular hydrophilic loop. In humans, OCT1 is primarily expressed in the liver while OCT2 is expressed in the kidney. OCT3 is expressed in the placenta, skeletal muscle, prostate, aorta and liver. OCT3, also known as extraneuronal monoamine transporter, is widely expressed in different regions of the brain including the hippocampus, cerebellum and cerebral cortex. OCT3 mediates the uptake of several neuroactive agents, including dopamine, and may play an important role in the disposition of neurotransmitters and cationic neurotoxins in the brain. The genes encoding human OCT1–3 map to a conserved cluster at chromosome 6q26-q27.
Synonyms	POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding transcription factor 3, Oct-3, Oct-4
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors

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