DsbA-L Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/1000. Immunohistochemistry: 1/200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Full length recombinant Mouse DsbA-L protein.
Specificity	Specific for the ~25 kDa DsbA-L protein in Western blots of 3T3-L1 adipocytes and Mouse white and brown adipose tissue lysate samples. It is suggested that you add 1% BSA to the antibody dilution buffer.
Formulation	State: Serum State: Liquid Serum in 40% Glycerol.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution (Gao F et al., 2009). Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis (Liu et al., 2008).
Synonyms	Disulfide-bond-A oxidoreductase-like protein
Reference Data

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