Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: M20]

Specifications

Product Data
Clone Name	M20
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: 1/80, preferable in PBS. Immunohistochemistry on Paraffin Sections: 1/5-1/25 after TUF treatment.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytokeratin purified from the Human breast carcinoma cell line MC-7
Specificity	This Monoclonal Antibody Clone M20 does stain almost all epithelial cells. In immunoblotting experiments the antibody recognizes only Keratin 8 (52,2 kD) and does not cross react with other cytokeratins. Frozen sections give excellent results in immunoperoxidase and immunofluorescence tests. Cross reactivity: Vimentin : not detectable Desmin : not detectable GFAP : not detectable Neurofilament : not detectable.
Isotype	IgG1
Formulation	State: Supernatant State: Liquid Culture Supernatant Preservative: 10mM Sodium Azide, 1% FCS
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data

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