B-Cells Mouse Monoclonal Antibody [Clone ID: MB2]

Specifications

Product Data
Clone Name	MB2
Applications	IHC, WB
Recommend Dilution	Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: For use in paraffin embedded tissue no antigen preteatment is needed but better results could be obtained if a TUF pretreatment is preformed with an antibody dilution of 1/20. Recommended Dilutions: Working dilution for regular immunohistochemistry is approximately 1/5-1/40, but the optimal dilution should be tested by serial dilutions. Recommended Positive Control: Tonsil, Lymph nodes. Incubation time: 60 min at RT.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Isolated from Human lymphocytes
Specificity	The antibody MB2 is an excellent Marker for lymphoma diagnosis. This MB2 antibody reacts with all B-cells. Further positive reaction with epithelia and endothelium was observed. MB2 recognizes neuraminidase-resistant cytoplasmic 28 kDa antigen, expressed strongly on B-cells and weakly on mature T-cells; no reaction with immature T-cells; negative staining with plasmacytomas.
Isotype	IgG1
Formulation	0.09% Sodium Azide State: Supernatant State: Liquid Culture Supernatant
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Reference Data

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