MIG (CXCL9) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	ELISA: 0.5-2.0 µg/ml (100 µl/well) will detect 0.2-0.4 ng/well of MIG.  To detect Human MIG by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIG (Cat.-No PP1055B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIG. Western Blot: 0.1-0.2 µg/ml will detect 1.5-3.0 ng/lane under reducing or non-reducing conditions. Neutralization: 5-7 µg/ml will neutralize 50% of the biological activity of 100 ng/ml of MIG. Immunohistochemistry on Frozen Sections or cells has been described: 1 µg/ml recommended for starting dilution. Immunohistochemistry on Paraffin Sections: 0.25-0.50 μg/ml with an overnight incubation at 4°C.  This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified (>98%) E.coli derived recombinant Human MIG.
Specificity	Reacts with Human Monokine Induced by Interferon-gamma (MIG). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 9
Database Link	Entrez Gene 4283 Human UniProt ID Q07325
Background	MIG (monokine induced by interferon-gamma), a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. MIG selectively chemoattracts Th1 lymphocytes, and also exerts other activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. Human MIG is active on murine cells.
Synonyms	C-X-C motif chemokine 9, CXCL9, CMK, MIG, SCYB9
Note	Centrifuge vial prior to opening!
Reference Data

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