Osteoprotegerin (TNFRSF11B) Rabbit Polyclonal Antibody

CAT#: PP1107P1

Osteoprotegerin (TNFRSF11B) rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



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CNY 3,910.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution Sandwich ELISA: To detect Human OPG by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5–2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated anti-Human OPG antibody (Cat.-No PP1107B1 or PP1107B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human OPG.
Western Blot To detect Human OPG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human OPG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: This antibody stained Formalin-Fixed Paraffin-Embedded Sections of Human breast invasive ductal carcinoma.
Use at 0.5 µg/ml with overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly purified (> 98%) E. Coli derived recombinant Human OPG (Cat.-No PA159)
Specificity Recognizes Human Osteoprotegerin (hOPG). 
Other species not tested.
Formulation PBS, pH 7.2 without preservatives
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing immobilized hOPG matrix
Conjugation Unconjugated
Storage Condition

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Gene Name tumor necrosis factor receptor superfamily member 11b
Background Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.
Synonyms OPG, OCIF
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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