Blood Group Lewis b Antigen Mouse Monoclonal Antibody [Clone ID: 2-25LE]

Specifications

Product Data
Clone Name	2-25LE
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot. Immunohistochemistry on Paraffin Sections: 5-10 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 min. Postive Control: Human colon adenocarcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).
Specificity	The antibody 2-25LE reacts with Lewis b blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. It does not react with the blood group H type 2 antigens or with Lewis A, Sialyl Lewis A, Lewis X, Sialyl Lewis X, Lewis Y, and Lewis C.
Isotype	IgG1
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for 6 months or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles.
Background	Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Synonyms	Blood Group Antigen Lewis b
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.