Rb (RB1) Mouse Monoclonal Antibody

Specifications

Product Data
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	The recombinant protein from 703aa-772 aa of human Retinoblastoma.
Isotype	IgG1
Formulation	This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Purification	The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	RB transcriptional corepressor 1
Database Link	NP_000312 Entrez Gene 5925 Human UniProt ID P06400
Background	The retinoblastoma (RB), a tumor suppressor protein, is a negative regulator of the cell cycle. This protein stabilizes constitutive heterochromatin to maintain the overall chromating structure. It can be inactivated by phosphorylation during G1/S stage by one or more cyclin-dependent kinases (CDKs). Different phosphorylation sites of RB may require more than one different CDKs which leads to a differential regulation of downstream effectors’ pathways. In the hypophosphorylated state, pRB is active and binds transcription factor E2F1, carries out its role as a tumor suppressor by inhibiting cell cycle progression. Phosphorylation by CDks inactives pRB which causes childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. pRB is widely expressed in a variety of human tissues including mammary carcinoma, esophageal cancer, squamous cell carcinoma and cervical cancer.
Synonyms	OSRC; p105-Rb; pp110; PPP1R130; pRb; RB
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.