Pcca (NM_144844) Mouse Untagged Clone
CAT#: MC200415
Pcca (untagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), (10ug)
CNY 5312.00
Product images

Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | C79630 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC006915 sequence for NM_144844
CGGACGCGTGGGGCCGGCAAGTCTGCGGGGTGGACGATGGCGGGGCAGTGGGTCAGGACCGTGGCGCTGT TGGCGGCCAGGCGGCATTGGCGGCGGTCCTCGCAGCAGCAATTGCTGGGGACGCTGAAGCATGCTCCAGT CTATTCATACCAATGCCTAGTGGTGTCCAGAAGTCTCAGTTCTGTGGAATATGAGCCTAAAGAAAAGACT TTTGATAAAATTCTCATTGCTAACAGAGGAGAAATTGCCTGTAGGGTTATTAAAACTTGCAAGAAGATGG GCATCAAGACAGTTGCCATTCACAGTGATGTTGATGCCAGTTCTGTTCACGTGAAAATGGCGGATGAGGC TGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTACCTCAACATGGATGCCATCATGGAAGCCATTAAG AAAACCAGGGCCCAAGCTGTACACCCAGGGTATGGATTCCTGTCAGAAAACAAAGAGTTTGCAAAGCGTC TGGCAGCAGAAGATGTCACTTTCATTGGACCTGATACTCATGCTATTCAAGCCATGGGTGACAAGATAGA AAGCAAACTATTAGCCAAGAGAGCAAAGGTCAACACAATCCCTGGTTTTGATGGGGTAGTAAAGGATGCA GATGAAGCTGTCAGAATTGCAAGGGAAATTGGCTACCCTGTGATGATCAAGGCCTCAGCAGGCGGTGGTG GGAAAGGCATGCGCATCGCCTGGGATGACGAAGAGACCAGGGATGGCTTTAGATTTTCATCCCAGGAAGC TGCTTCTAGTTTTGGTGATGATAGACTACTAATAGAAAAATTTATCGATAACCCTCGTCATATAGAAATC CAGGTTTTAGGGGATAAACATGGCAATGCTCTGTGGCTCAATGAGAGGGAGTGCTCGATCCAGAGAAGGA ACCAGAAGGTGGTAGAGGAGGCGCCAAGCATTTTTCTGGATCCTGAAACTCGCCAAGCAATGGGAGAGCA GGCCGTGGCTTTGGCTAAAGCCGTGAAGTATTCTTCTGCTGGAACTGTGGAATTTCTTGTGGACTCCCAG AAGAATTTTTACTTCTTGGAGATGAATACAAGACTACAGGTCGAACATCCTGTCACAGAGTGCATTACTG GCCTGGACTTAGTCCAAGAAATGATCCTTGTTGCTAAGGGTTACCCACTCAGGCACAAGCAAGAGGATAT TCCCATCAGTGGCTGGGCAGTTGAATGTCGGGTTTATGCTGAGGACCCCTACAAGTCTTTCGGTTTACCG TCTATTGGGAGGCTGTCCCAGTACCAAGAGCCGATACATCTACCTGGTGTCCGAGTTGACAGTGGCATCC AACCAGGAAGTGACATCAGCATCTATTATGATCCTATGATTTCAAAGCTAGTCACATATGGGTCTGACAG AGCAGAAGCCCTGAAGAGGATGGAAGACGCACTGGACAATTATGTGATCCGGGGTGTTACACACAACATC CCATTGCTCCGGGAGGTGATAATCAACACACGTTTTGTGAAAGGAGACATCAGCACTAAGTTTCTCTCTG ATGTGTATCCTGATGGCTTCAAAGGGCACACGTTAACACTGAGTGAGAGAAACCAGTTATTGGCCATTGC ATCATCTGTATTTGTGGCATCCCAGCTACGAGCTCAGCGCTTCCAAGAACGTTCAAGAGTACCAGTTATT AGGCCTGATGTGGCTAAGTGGGAGCTCTCGGTAAAGTTACATGATGAAGATCATACTGTCGTGGCATCTA ACAATGGGCCGGCATTTACCGTGGAAGTTGATGGCTCGAAACTAAATGTGACCAGTACGTGGAACCTGGC GTCACCCTTATTGTCTGTCAACGTTGATGGCACGCAGAGGACTGTGCAGTGTCTTTCTCGGGAAGCAGGT GGAAACATGAGCATCCAGTTTCTTGGCACAGTGTACAAAGTGCACATTTTAACCAAGCTTGCTGCAGAGC TGAACAAATTCATGCTTGAAAAAGTGCCCAAGGACACCAGCAGCACTCTGTGCTCCCCGATGCCTGGAGT GGTGGTGGCCGTTTCTGTCAAGCCTGGAGACATGGTAGCAGAAGGTCAGGAAATCTGTGTGATTGAAGCT ATGAAAATGCAGAACAGTATGACAGCTGGGAAAATGGGCAAGGTGAAATTGGTGCACTGCAAAGCTGGAG ACACAGTTGGTGAAGGAGACCTGCTTGTGGAGCTGGAATGAAGTATTTCTAGCCTTTCCGTCAGCACTGC TCACTGCTTTAACTGGCGTCTCCATTATTCTCTAACCCTCAATTTGCGCAAGCATTTACAGGAGCACTCC TGTGCAGGAGGTTCTACAGCCTCCTGTTTATTCCTCAGAAGAGGCAAAACCGACATTCTGCCAACAAATC ACCAATGGAAATTCTTGATATAAATACTTGTACATATGATTTGTACTTCTGCTGAGATTTCCTAGTGTCA AAATTAAATCAATAAAACCAAGCATTTGTCTAAAAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | NM_144844 |
Insert Size | 2175 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC006915, AAH06915 |
RefSeq Size | 2499 bp |
RefSeq ORF | 2175 bp |
Locus ID | 110821 |
UniProt ID | Q91ZA3 |
Gene Summary | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG210302 | Pcca (tGFP-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) |
CNY 6370.00 |
|
MR210302 | Pcca (Myc-DDK-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) |
CNY 5304.00 |
|
MR210302L3 | Lenti ORF clone of Pcca (Myc-DDK-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) |
CNY 7700.00 |
|
MR210302L4 | Lenti ORF clone of Pcca (mGFP-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) |
CNY 7700.00 |