Rpusd4 (NM_028040) Mouse Untagged Clone
CAT＃: MC211270
Rpusd4 (untagged) - Mouse RNA pseudouridylate synthase domain containing 4 (Rpusd4), (10ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2410001E19Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC211270 representing NM_028040
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGCCCTGCTTGCGTACGCCTGGTGTCCAGCTTTTGAGTATGAGTTCGCGTCCCGGGAGATTGT TCACTCCCGGCTCCTGGTCGTTTTGTTCCAGCGCTACTTCCTCTAGGCCCCTGAATGCTCAGAGATTGGC AGAGAAGCTCCGAGCCCAGAAACAAGAGCAAAAGGCAAAGGAAGTGCGGGTGCCTACAAACCCCGTTCAG CGGAGAGTGCAGGAACTAGTGCGATTCACACAGCAGCTGCAGCGAGTTCACCCCAATGTGCTTGCTAAGG AACTGAGCCGAAGGATTCTCCACCAGGACAACGACCTCGTGGTCATTAATAAACCCTATGGTCTCCCTGT CCATGGTGGCCCAGGAGTCCAGCTCTGCATCAGTGATGTACTGCCCATCTTGGCAAAGATGCTTCATGGC CACAAGGCAGAACCTTTGCATCTGTGCCACCGTCTGGACAAAGAAACCACAGGTGTAATGGTTTTGGCTT GGGAGAAGGACATGGCACATCAAGTCCAGGAGCTATTTAGAACCCGCCAGGTGGAAAAGAAGTACTGGGC CATCACGGTGCGTGTTCCCTTGCCCTCGGCAGGAGTCGTGGATATCCCCATCAAGGAGAAAGAAGTGCAG GGGCCTCAGCAACACCACAAGATGACGCTGTCCCCAAGTTACCGTCTGGACAATGGGAAAATGGTAAAGG TACGGGCCAGCCGCGATGCGCACGTTGCTGTAACGCAGTACCAGGTGCTTAGCAGCGCTTCCTCCTCTGC CCTTGTGGAGCTCCAGCCTGTTACTGGAATAAAGCATCAGCTCCGAGTTCACCTGTCCTTTGGGCTGGAT TGCCCAATCCTTGGGGATCACAAGTACTCAGACTGGACTAGGTTGGCCCCTCAGAAACTTTCTGCTGGCA CACTGAAGAAGCTGGGGCTACAGCAGTCAAAGGCCCGCTACATCCCGCTTCACCTGCACGCCAGGCAGCT CATCCTGCCTGCTCTGGGCTCCAGGACAGAGGAACTCCTCCTGACCTGCAAGCTTCCTCACTTCTTTGCA CGTTCCCTGCTCCGACTGGGTTTAGATATGCCAAATCAGGATCAAAGCCGAGGCAATAAGGCCAGGCATG TGGAAGCACGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028040 |
| Insert Size | 1134 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028040.2, NP_082316.1 |
| RefSeq Size | 1827 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 71989 |
| UniProt ID | Q9CWX4 |
| Gene Summary | Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome. Acts on position 39 in mitochondrial tRNA(Phe). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205860 | Rpusd4 (tGFP-tagged) - Mouse RNA pseudouridylate synthase domain containing 4 (Rpusd4) |
CNY 4370.00 |
|
| MR205860 | Rpusd4 (Myc-DDK-tagged) - Mouse RNA pseudouridylate synthase domain containing 4 (Rpusd4) |
CNY 5488.00 |
|
| MR205860L3 | Lenti ORF clone of Rpusd4 (Myc-DDK-tagged) - Mouse RNA pseudouridylate synthase domain containing 4 (Rpusd4) |
CNY 5890.00 |
|
| MR205860L4 | Lenti ORF clone of Rpusd4 (mGFP-tagged) - Mouse RNA pseudouridylate synthase domain containing 4 (Rpusd4) |
CNY 5890.00 |
