Dtd2 (NM_029545) Mouse Untagged Clone
CAT＃: MC214512
Dtd2 (untagged) - Mouse RIKEN cDNA 6530401N04 gene (6530401N04Rik), (10ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4930578F06Rik; 6530401N04Rik; B830049N13Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC214512 representing NM_029545
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGGTGGTCGTGTGGCCCAGGCTCGTGCGCTCCTGCAGCAGTGCCTGCACGCTCGCCTGCAAG TGCGCCCGGCCGATGGAGACGCCGCGGCGCAGTGGGTGGAGATTCGGAGAGGATTGGTGATCTATGTGTG TTTCTTCAAGGGAGCTGACACAGACCTTCTCCCCAAAATGGTTAATACGCTGTTAAATGTGAAGTTAAGC GAAACGGAAACCGGCAAGCACGTCTCCATCCTCGATCTGCCTGGCGATGTTCTGATCATCCCGCAAGCCA CCCTCGGGGGCAGGGTGAAAGGGCGGAGCATGCAGTACCACTCTAACTCTGGAAAGGAGGAGGGCTCAGA ACTGTACTCCCAGTTTGTGAGTCTGTGTGAAAAAGCCGTGGCCAACAACACCAAGAGTGTAGAAGCGGGG GTTGCGGTGGCGCACGGCACGTACGGGAACAGGCAAGTGTTAAAGCTGGACACCAATGGACCCTACACGC ACCTCATTGAGTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_029545 |
| Insert Size | 507 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_029545.2, NP_083821.1 |
| RefSeq Size | 2564 bp |
| RefSeq ORF | 507 bp |
| Locus ID | 328092 |
| UniProt ID | Q8BHA3 |
| Gene Summary | Deacylates mischarged D-aminoacyl-tRNAs. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS. Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) is longer and has a distinct C-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220756 | Dtd2 (tGFP-tagged) - Mouse RIKEN cDNA 6530401N04 gene (6530401N04Rik), (10ug) |
CNY 2850.00 |
|
| MR220756 | Dtd2 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6530401N04 gene (6530401N04Rik) |
CNY 2400.00 |
|
| MR220756L3 | Lenti ORF clone of Dtd2 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6530401N04 gene (6530401N04Rik) |
CNY 4750.00 |
|
| MR220756L4 | Lenti ORF clone of Dtd2 (mGFP-tagged) - Mouse RIKEN cDNA 6530401N04 gene (6530401N04Rik) |
CNY 4750.00 |
