Nme8 (NM_001167909) Mouse Untagged Clone

CAT#: MC218460

Nme8 (untagged) - Mouse thioredoxin domain containing 3 (spermatozoa) (Txndc3), transcript variant 2, (10ug)



  "NM_001167909" in other vectors (3)

CNY 6940.00


货期*
4周

规格
    • 10 ug

Product images

推荐一起购买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1700056P15Rik; Sptrx-2; Txndc3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC218460 representing NM_001167909
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGCAAAAAGCGTGAAGTCCAGCTACAGTCAGTCGTCAATAGTCAGAACTTGTGGGATGAGATGT
TGCTGAACAAAGGCTTAACAGTGATTGATGTTTACCAAGCCTGGTGTGGACCTTGCAAAGCCGTGCAAAG
TTTATTCAGAAAACTAAAAAATGAACTGAACGAAGATGAGATTCTTCACTTCGTCGTTGCTGAAGCTGAC
AACATTGTGACTCTCCAGCCATTTAGAGATAAATGTGAGCCCGTGTTTCTCTTTAGTCTTAATGGTAAAA
TCATTGCAAAGATTCAGGGTGCAAATGCTCCACTTATCAATAGAAAAGTCATTACCTTGATAGATGAAGA
GAGGAAAATTGTAGCAGGTGAAATGGATCGTCCTCAGTATGTTGAAATTCCACTAGTAGATGCAATCGAT
GAAGAATATGGGGAAGTACAGTATGAAAGTGCTGCGGAAGTTTACAATATGGCAATTATCAAACCTGATG
CTGTACTCATGAGAAAAAATATAGAAGTTAGGGAAAAAATAGCCAAAGAAGGATTTGTTATAGAAATACA
AGAAAACCTGATTCTCCCTGAAGAGGTAGTGAGGGAATTCTACACTCATATAGCAGACCAGCCTGACTTT
GAAGAGTTTGTCGTTTCTATGACAAATGGCCTCAGCTGTGTGCTCATTGTATCTCAAGAAGACTCCGAGG
TTATTCAGGAAGAAACTCTCCCGCAGACTGATACAGAAGAAGAACCTGGCGTTTTGGAAGAGCCTCACGT
TAGGTTTGCACCTGTGATGATAAAGAAGAAACGGGACAGTTTGCAAGAGTATATGGACCGACAGCATATG
TCTGATTACTGCGATGTCGAGGACGATGCGGTTAAGGTCTCTAAGCTCATTGACATATTATTCCCTGATT
TTAAAACTATGAAAAGCACGAATGTACAAACGACGCTAGCATTACTGCATCCAGACATCTGTGAGGAAGA
GAAAGATGACGTGTTGAACGTTATTCACAATGAAGGGTTCACCATACTGATGCAGAGGCAAATCGTATTA
TCAGAGGAAGAAGCAAGAACAGTGTGCAAGATCCATGAAAACGAAGAGTATTTTGATAATCTTATAGGGC
ACATGACCAGTAATCACTCTTATGTCCTTGCTCTACGGAGGGAAAATGGTGTGGAATATTGGAAAACATT
AATTGGGCCAAAAACGATTGAGGAAGCTTATGCATCTCATCCACAGAGTTTATGTGTACAGTTTGCTTCA
GGGAATTTTCCTACCAACCAGTTCTACGGGAGCAGTTCAAAAGCAGCAGCTGAGAAGGAAATAGCGCATT
TCTTCCCTCCCCAGAGCACACTTGCATTGATCAAGCCTCATGTGACACACAAAGAAAGAATTCACAGAAG
CTCAAGGAGGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001167909
Insert Size 1413 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001167909.1, NP_001161381.1
RefSeq Size 1704 bp
RefSeq ORF 1413 bp
Locus ID 73412
UniProt ID Q715T0
Gene Summary Probably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment near the C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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