Col9a3 (NM_009936) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV006866
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC220406 representing NM_009936 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGGAGCGCCTGCCCTAGCCCTGCTGCTGCTGGGGCAGCTCCTGACGGCCACCTCCGCGCAGAAAG TGGGACCCCGAGGCCCCCCTGGTCCCCAAGGGCCTCCTGGAAAACCCGGCAAGGATGGCATTGATGGAGA AGCTGGCCCTCCAGGTCTGCCTGGCCTTCCAGGACCCAAAGGGACCTCAGGGAAGCCAGGGAAGCCGGGA GAGGCAGGACTGCCAGGACTGCCTGGTGTAGATGGTCTGACAGGGAGAGATGGACCCGCAGGACCCAAAG GTGCCCCTGGAGAACGGGGAAGTCTAGGACCCCCAGGGCCACCAGGACTTGGGGGCAAAGGCCTCCCTGG ACCTCCTGGAGAGGCAGGAGTGAGTGGCCTCCCAGGTGGGATTGGTCTACGTGGCCCCCCGGGACCCTCT GGACTTCCAGGACTGCCTGGCCTCCCAGGACCTCCTGGACCTCCTGGAAACCCTGGAGTCCTCCCTGAAG GTGCTACTGATCTGCAGTGTCCCGCCATCTGCCCGCCAGGCCCTCCGGGACCCCCAGGAATGCCGGGGTT CAAGGGGCCTACTGGCTACAAAGGGGAACAAGGAGAAGTTGGCAAAGATGGTGAGAAGGGTAGTCCTGGC CCCCCCGGGCCTCCTGGAATCCCAGGCACCGTGGGGCTACAGGGCCCACGAGGATTAAGAGGACTTCCAG GGCCACTCGGGCCCCCTGGGGACCGGGGTCCCATTGGGTTTCGGGGCCCCCCTGGGACCCCAGGAGCACC TGGGAAAGTGGGTGACAGGGGTGAAAGGGGACCAGAAGGGTTCCGTGGCCCTAAGGGTGACCTGGGCAGG CCTGGTCCCAAAGGAATCCCTGGAATGGCTGGGCCAGGCGGAGAACCAGGCATGCCAGGCAAGGATGGCA AAGATGGTGTGCCGGGACTTGATGGTGAGAAGGGAGAGGCTGGTCGCAATGGTGGCCAAGGAGAGAAAGG CCCCAATGGGCTGCCGGGGCTCCCTGGACGAGCAGGGTCCAAAGGCGAGAAGGGAGAACCGGGTAGAACT GGCGAGCTGGGTGAGGCTGGCCCCTCTGGAGAGCCAGGTATCCCTGGAGATGTTGGTGTTCCAGGGGAGC GTGGTGAGGCTGGTCACAGGGGCTCCGTGGGAGCTCTTGGCCCACAAGGTCCTCCTGGTGCTCCTGGCAT CCGTGGCTTTCAGGGACAAAAAGGCAGCACAGGAGACCCTGGCCTTCCGGGCCCCCAAGGCCTACGGGGA GATGTGGGTGACCGGGGTCCAGGAGGTGCCACAGGCCCTAAGGGAGACCAGGGCATTGCAGGTTCCGATG GTCTTCCAGGGGACAAAGGCGAGCTGGGTCCTAATGGCCCCGTTGGACAAAAAGGAGAGTCTGGCAGCCG AGGGGAACTGGGCCCCAAAGGCATCCAGGGCCCCAATGGCACCAGTGGAGTCCAGGGTGTACCTGGTCCC CCAGGTCCACTGGGCCTCCAAGGTGTGCAGGGTGTCCCAGGCATCACCGGGAAGCCTGGAGTTCCGGGCA AGGAAGCCAGTGAACAGCGCATCAGGGAGCTATGCGGGGGTATGATCAGTGAGCAAATTGCACAGTTGGC TGCACACCTGAGGAAGCCCTTAGCACCAGGCTCCATCGGCAGGCCTGGTCCAGCTGGCCCCCCAGGCCCT CCAGGCCCTCCAGGCTCTATTGGCCACCCTGGCGCTCGGGGTCCCCCTGGATACCGTGGTCCCACTGGGG AACTAGGAGATCCTGGACCAAGAGGGAGCCAGGGTGACAGAGGGGACAAGGGAGCAACAGGTGCAGGGCT GGATGGGCCTGCTGGGGACCAGGGCTACCAAGGGCCTCAAGGTGTACCTGGCATCAGCAAAGATGGTCGA GATGGTGCTCATGGTGAGCCTGGCCTTCCCGGTGATCCTGGCCTTCCTGGAGCTGCTGGTGCTCAGGGTA CCCCAGGGATCTGCGACACTTCAGCCTGCCAAGGAGCTGTGTTAGGGGGAGGTGGGGAAAAGTCGGGTCC TCGGAGCTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009936
Insert Size	2043 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009936.2, NP_034066.2
RefSeq Size	2846 bp
RefSeq ORF	2043 bp
Locus ID	12841

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