Mthfd1l (NM_172308) Mouse Untagged Clone
CAT＃: MC222931
Mthfd1l (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)
CNY 12540.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2410004L15Rik; AI647056; Fthfsdc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222931 representing NM_172308
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGTCCGCCTCCCTCTCCTGCTTCGCCAGCTCGGCCGCCAGCAGCTGCCCTCGGGCCCGGCGTGTC GCCTCCGTGAGCTGTGTCGCTCAGGCAGCAGAAGCAGCAGCAGCGGAGGTGGTGACCCCGAGGGGTTACG CGGGCGGCGGCTGCAGGATGGCCAAACCTTCAGCAGCCATGGCCCAGGCAATCCGGAAGCCCCCGGGATG GACTCCATCGTCAGAGACGTCATTCACAATTCCAAGGAAGTGCTGAGCTTGTTGCAAGAAAAGAACCCTG CCTTTAAGCCAGTGCTTGTGGTTATTCAGGCCGGTGATGACAATTTGATGAAGGATATGAACCAGAATTT GGCCAAGGAGGCTGGTCTGGACATCACTCACATCTGCTTGCCTCCAGACAGCGGAGAGGATGAGATCATA GATGAAATCCTGAAGATTAATGAAGACCCCAGAGTGCATGGCCTTACCCTTCAGATCTCTGAAGATTCAT TGAGCAACAAAGTTCTCAATGCTTTGAAACCAGAAAAAGATGTGGACGGGGTGACCGACATCAATCTGGG GAAGCTCGTACGAGGTGACGCTCCGGAGTGTTTTGTTTCACCCTTGGCCAAAGCTGCTGTAGAACTTGTT GAAAAGTCAGGGATCACTTTGGATGGAAAGAAGGTCTTGGTCGTAGGGGCGCACGGGCCCCTGGAAGCTG CCCTGCAGTGGCTTTTCCAGAGAAAAGGATCTATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCC AGACAAGCTTCGAGAGGCAGACATTGTGGTCTTAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGTGTGG ATTCCATCAGGAACCACCATTCTCAATTGCTTCCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTC CTGGGGTCCCCGTGGACAAACTCATTGCAGAAGAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCA GAACATGGTGAGCAGCGGCAGGAGGTGGCTCAGAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTG AAACTGCAGCCTCTCTCACCTGTGCCCAGTGATATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGG ATGTCCTTGCCAAGGAGATAGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGT CCAATTGTCCCTGCTGGAGAGGTTAAAGGATCAAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACG CCCACCCCGCTCGGAGAAGGGAAGAGTACAGTCACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGA AAGTCAACTCCTTCGCCTGTCTGAGGCAGCCTTCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGC AGGAGGTGGATATGCTCAGGTCATCCCCATGGAGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCC ATCACTGCCGCTAATAACTTGCTGGCCGCAGCCATCGACACGAGAATTTTACATGAGAGCACTCAAACAG ACAAGGCTCTCTACAATCGACTGGTTCCCTTAGTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTC TCGGCTGAAGAAACTGGGGATACATAAGACTGATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTC GCCCGCCTCAACATTGACCCCGCAACCATCACGTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGA GAAAAATAACCATTGGACAGGGAAGTACAGAGAAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGT GGCCAGCGAGATCATGGCCGTGCTGGCCTTGACTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGA ATGGTGGTGGCCAGTGACAAAGACGGGCAGCCTGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGA CAGTTTTGATGAAAGACGCCATCAAGCCAAATCTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCA CGCTGGTCCCTTTGCCAACATCGCTCACGGCAACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTG GTCGGCGAAGAAGGGTTTGTGGTGACTGAAGCTGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCA ATATCAAATGCCGAGCTTCTGGCCTGGTGCCCAACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAA GATGCATGGAGGCGGGCCGAGTGTAACTGCTGGGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATT CAGCTGGTAGCTGATGGCTGCTGCAACCTACAGAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGG TGGTGGTGGCACTGAATGTCTTCAAGACGGACACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAA ACGGGCCGGTGCCTTTGATGCGGTCCCCTGCTATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTG GCCCGGGCTGTGAGAGAAGCTGCGAATAAAAGAAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCA TCGTGGAAAAGATAAGAGTCATTGCCCAGACTGTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGC GCAGTCCAAAATCGATCGCTATACGCAACAGGGTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCAC CTCTCTCTCTCTCATGAACCTGACAAAAAGGGAGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCC GGGCCAGCATCGGGGCTGGGTTCATTTATCCTTTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCAC CCGACCCTGCTTTTATGATATAGACCTTGACACAGAAACGGAGCAAGTTAAAGGCTTGTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172308 |
| Insert Size | 2934 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172308.4, NP_758512.3 |
| RefSeq Size | 3731 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 270685 |
| UniProt ID | Q3V3R1 |
| Gene Summary | May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221835 | Mthfd1l (tGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug) |
CNY 10740.00 |
|
| MR221835 | Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9790.00 |
|
| MR221835L3 | Lenti ORF clone of Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 11690.00 |
|
| MR221835L4 | Lenti ORF clone of Mthfd1l (mGFP-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 11690.00 |
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