Phlpp (BC059254) Mouse Untagged Clone
CAT＃: MC223110
Phlpp (untagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203), (10ug)
CNY 12920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | SCOP, mKIAA0606 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC059254
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTGCCAGTGAACCGATGGACAAGACGACAGGTCATTCTGTGCGGGACGTGCTTGATAGTGTCGT CCGTGAAAGACAGCGTGAGTGGGAAGATGCATGTCCTGCCCCTCATTGGTGGAAAAGTGGAGGAAGTGAA AAAGCACCAGCACTGTTTAGCGTTCAGCTCTTCGGGGCCTCAAAGCCAGACTTACTACATCTGCTTTGAT ACTTTCACGGAGTACTTACGGTGGCTGCGGCAAGTCTCCAAGGTTGCATCACAGCGTATAAGCTCAGTAG ACCTCTCCTGTTGTAGCCTTGAACATCTGCCTGCCAACCTCTTTTACAGCCAAGACCTTACTCATCTCAA CTTAAAACAGAACTTCCTAAGGCAAACCCCCACCCTCCCAGCTGCCAGAGGACTTGGTGAACTGCAAAGG TTCACCAAATTGAAGAGCCTTAACCTTTCCAATAACCACCTAGGAGCCTTTCCGTCAGCAGTCTGCAGCA TTCCAACCCTAGCAGAGCTGAATGTATCTTGCAATGCCCTGCGAGAAGTCCCAGCAGCTGTTGGAGATAT GCAGAACTTGCAGACGTTTCTGCTGGATGGAAATTTTCTCCAGTCCCTTCCTGCTGAACTGGAGAGCATG CACCAGCTCAGCTATCTGGGTCTTTTCTTTAATGAATTCACTGACATTCCAGAGGTATTGGAGAAGCTGA CTGCTGTGGATAAGCTGTGTATGGCTGGGAACTGTGTGGAGACCCTCAGACTACAGGCCTTAAGAAGAAT GCCTCATATTAAACATGTGGACCTAAGACTGAACATACTCAGAAAGCTTATGGCAGATGAGGTGGACTTT GTGCAACATGTCACTCAGCTTGACCTGCGAGACAATAAGCTTGGTGATCTAGATGCTATGATCTTCAACA ACATAGAAGTTCTGCACTGTGAGAGGAATCAGCTGGTGACATTGAACGTTTGTGGCTATTTCCTAAAGGC ACTCTATGCTTCTTCTAATGAACTTGCTCAACTTGATGTCTACCCAGTTCCGAATTATCTCTCTTACATG GATGTCTCAAGAAACTGCCTAGAAAGTGTGCCTGAGTGGGTATGTGAAAGCCGGAAATTAGAAGTTTTGG ATATTGGCCATAATCAAATATGTGAACTTCCTGCCCGCCTGTTTTGTAATAGTAGTCTCCGGAAATTGCT AGCAGGACACAACCGGTTGGCAAGGCTTCCTGAAAGGCTGGAGAGAACTTCTGTGGAGGTCTTGGATGTC CAGCACAACCAGATCACTGAGCTCCCACCAAACCTCCTCATGAAGGCTGACAGCCTGAGATTCCTGAATG CATCTGCAAACAAACTGGAAACCCTGCCTCCAGCCACACTTTCTGAGGAGACGAGCAGTATATTACAGGA GTTGTACCTGACAAACAACTGCCTCACGGATAAGTGTGTGCCCTTGTTAACAGGGCACCCCCGTCTGAAG ATCCTACACATGGCCTATAACCGGCTTCAGAGCTTCCCGGCAAGTAAAATGGCAAAACTGGAGGAACTTG AAGAAATTGATATCAGTGGGAATAAGCTGAAAGCCATCCCCACAACAATCATGAACTGCAGACGCATGCA CACCGTGATTGCTCACTCCAATTGTATCGAAGTCTTTCCTGAAGTTATGCAGCTCCCAGAAGTCAAGTGT GTAGATCTGAGCTGTAATGAGCTGAGTGAAATCACGTTACCAGAAAACCTGCCACCTAAGCTGCAGGAGC TAGACCTGACTGGAAATCCACGCCTTGCCCTGGACCACAAAAGCCTGGAGCTGCTCAATAACATTCGCTG CTTCAAGATTGACCAGCCGTCAGCAGGAGATGCATCAGGAGCCCCAGCAGTATGGAGTCATGGTTACACT GAAGCATCGGGAGTAAAAAACAAGCTGTGTGTCGCAGCCCTGTCTGTAAATAACTTCCGTGACAACCGAG AGGCCCTCTATGGTGTGTTTGATGGAGACCGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACCATGAG TGACATTTTGGCTGAAGAGCTTCAGAAAACGAAAAATGAAGAAGAATACATGGTCAATACATTCATAGTC ATGCAAAGGAAACTGGGAACTGCTGGGCAGAAACTTGGTGGTGCTGCTGTCCTCTGCCACATCAAGCCTG ACCCTGTGGACCTGGGAGGATCCTTTACCCTGACCTCTGCTAATGTTGGCAAGTGCCAAACGGTTCTCTG TCGAAATGGGAAGCCACTGTCTCTGTCCAGGTCATACATCATGAGCTGTGAAGAAGAGCGGAAGAGGATT AAGCAGCACAAAGCCATCATCACTGAGGATGGCAAGGTCAATGGAGTGACAGAGTCCACGCGCATCCTGG GCTACACCTTCCTCCATCCCAGTGTGGTGCCTCGCCCCCATGTGCAGTCGGTGCTTCTGACTCCACAGGA TGAGTTTTTCATCCTGGGCAGCAAAGGGCTGTGGGACAGCCTATCCATTGACGAGGCTGTGGAAGCTGTA CGCAATGTGCCAGATGCTCTGGCTGCTGCTAAGAAGCTCTGCACCCTGGCTCAGAGCTATGGCTGCCATG ACAGCATCAGTGCTGTGGTGGTACAGCTAAGTGTCACCGAAGACAGCTTCTGCTGCTGTGAGCTCAGTGC TGGAGGGAGCATGCCACCACCCAGCCCCGGAATCTTCCCACCATCCGTCAACATGGTCATCAAGGACCGA CCCTCAGATGGGCTGGGTGTGCCATCCTCCAGCAGTGGCATGGCATCTGAGATCAGCAGTGAACTGTCTA CCTCCGAGATGAGTAGTGAGGTGGGCTCCACAGCCTCTGATGAGCCCCCGTCTGGAGTCCTGAATGAGAG CAGCCCTGCCTACCCCAACGAGCAGCGCTGCATGCTCCACCCTGTCTGCCTGTCTAACTCCTTCCAACGT CAGCTGTCCAGCGCTACTTTCTCCAGCGCGTTCTCTGACAACGGCCTTGACAGTGACGATGAAGAACCCA TTGAGGGGGTGTTCAGCAACGGCAGCCGGGTTGAGGTGGAAGTAGACATCCACTGCAGCAGGGCCAAGGA AAAGGAGAGACAGCAGCACCTACTTCAGGTGCCAGCTGAGGCCAGTGATGAGGGCATTGTCATCAGTGCC AATGAGGATGAGTCAGGTCTGTCCAAGAAGGCAGACTTTTCTGCTGTGGGAACCATTGGACGACGCAGGG CTAATGGCTCTGTAGCTCCCCAGGAAAGGAGCCATAATGTGATAGAGGTTGCTGCAGATGCGCCTCTCCG GAAGCCAGGAGGCTATTTTGCAGCCCCTGCTCAGCCAGATCCAGATGATCAGTTTATCATCCCCCCAGAG CTGGAAGAGGAAGTCAAAGAAATCATGAAACATCACCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC TGCCACCACCACCCCAGCCGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCG GCACTTCCAAATGGATCACCTGCCAGACTGTTACGATACACCGCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC059254 |
| Insert Size | 3549 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059254, AAH59254 |
| RefSeq Size | 4783 bp |
| RefSeq ORF | 3548 bp |
| Locus ID | 98432 |
| Gene Summary | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (By similarity). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (PubMed:17382888). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (PubMed:20080691). Involved in development and function of regulatory T-cells (PubMed:21498666).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211767 | Phlpp (tGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203) |
CNY 12920.00 |
|
| MR211767 | Phlpp (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203) |
CNY 11780.00 |
|
| MR211767L3 | Lenti ORF clone of Phlpp (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203) |
CNY 13680.00 |
|
| MR211767L4 | Lenti ORF clone of Phlpp (mGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203) |
CNY 13680.00 |
