Pum2 (NM_030723) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730503J23Rik; Pumm2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223422 representing NM_030723 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA GACTGCATGGGGAACTTCTCATCATTCAATGTCCCAGCCTATTATGGTGCAGAGAAGATCTGGACAGAGT TTTCATGGAAACAGTGAAGTAAATGCAATTCTTTCTCCACGCTCAGAAAGTGGAGGCCTTGGTGTGAGCA TGGTAGAATATGTATTAAGTTCCTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAACTTTTGG TACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG GACCAAAACAGAGATCTTAAACAAGATGATGAGGACTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAA TGGATGCCGATTGCAAAGATTTTAATCGCACTCCTGGAAGTCGCCAAGCCTCTCCAACTGAAGTAGTTGA GCGCCTTGGCCCTAGTACTAATCCCCCAGAAGGATTGGGCCCTCTTCCTAATCCGACAGCGAATAAACCA CTTGTTGAAGAATTTTCAAACCCTGAAACTCAGAATCTGGATGCAATGGACCAAGTTGGTCTGGATTCCT TACAGTTTGACTATCCTGGTAATCAGGTACCCATGGATTCTTCAGGAGCTACTGTAGGCCTTTTTGACTA CAATTCCCAACAGCAGCTCTTTCAGAGGACTAGTGCACTAACAGTTCAGCAGTTAACTGCAGCTCAGCAG CAGCAGTATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTCCAG CTGCATTTGTGCCAAATCCATATATTATTAGTGCTGCTCCTCCAGGGACTGACCCGTATACTGCAGCAGG ATTGGCTGCAGCAGCAACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGTGTTCCATGGGGA GTGTATCCAGCCAATTTATTTCAGCAACAAGCTGCAGCTGCGGCAAGCAACACAGCCAACCAGCAAGCAG CATCACAAGCTCAGCCTGGACAGCAGCAGGTTCTTCGTCCTGGAGCAGGTCAGCGTCCTATTACTCCAAG TCAGGGCCAACAAGGGCAGCAAGCAGAGTCACTTGCAGCAGCTGCAAACCCAACTTTGGCTTTTGGTCAG AGTCTTGCTGCAGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCT TAGTGGTTGGCCCCGGAGCAAGAACTGGCCTTGGAGCTCCAGTACGATTAATGGCTCCAACACCTGTCTT AATAAGTTCAACAGCAGCACAAGCTGCAGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAATAGTCTTACA GGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAGCAGCAGCAGCAAC CAAGCACTAACCTGCAGTCTAATTCATTTTATGGGAGCAGCTCTTTGACTAACAGCTCCCAGAGCAGCTC TTTATTCTCTCATGGACCTGGCCAACCTGGAAGTGCGTCTCTTGGCTTTGGAAGTGGTAGCTCTTTAGGA GCTGCTATAGGCTCAGCTCTCAGTGGATTTGGCTCATCAGTTGGCAGTTCTGCAAGTAGTAGTGCCACAA GGAGAGAGTCTCTATCTACTAGCTCTGACTTGTACAAAAGATCTAGTAGCAGCCTAGCACCCATAGGGCA ACCATTTTACAATAGTCTGGGATTTTCCTCCTCTCCAAGTCCAATAGGCATGCCTCTGCCAAGCCAAACT CCAGGACATTCACTTACGCCACCGCCATCACTTTCATCACATGGATCCTCATCCAGTTTGCATTTAGGAG GACTGACAAACGGTAGTGGTCGGTATATCTCTGCAGCACCTGGAGCAGAAGCAAAGTACCGAAGTGCTTC AAGCACTTCCAGTCTATTTAGCTCCAGCAGCCAGCTCTTTCCTCCTTCTCGGCTCCGCTATAATAGATCT GACATCATGCCCTCCGGCCGCAGTAGGTTATTGGAAGATTTCAGGAACAACCGCTTCCCCAACCTTCAGC TCAGAGACTTAATTGGACATATAGTCGAGTTTTCTCAAGACCAGCATGGTTCCAGATTCATACAGCAAAA GCTAGAGAGAGCTACTCCAGCTGAGCGACAAATAGTATTTAATGAAATTCTACAGGCAGCCTATCAATTA ATGACAGATGTTTTTGGAAACTATGTTATACAGAAGTTTTTTGAGTTTGGAAGTTTGGATCAGAAATTAG CCCTGGCTACTCGTATTCGTGGTCATGTTCTACCATTAGCCTTGCAGATGTATGGCTGCCGTGTTATTCA AAAGGCGTTAGAATCTATTTCTTCTGATCAGCAGGTAATTAGTGAAATGGTTAAGGAACTAGATGGCCAT GTACTTAAATGTGTGAAAGATCAAAATGGAAACCATGTTGTACAGAAATGCATTGAATGTGTTCAGCCAC AGTCACTGCAGTTCATCATCGATGCTTTCAAAGGACAAGTATTTGTGCTTTCAACCCATCCTTATGGCTG CAGAGTCATTCAGCGTATCTTAGAGCACTGCACGGCAGAGCAGACCTTACCCATCTTAGAAGAACTTCAC CAACACACAGAACAGTTGGTACAGGATCAGTATGGCAATTATGTTATTCAGCATGTACTGGAACATGGTC GACCTGAAGACAAGAGCAAAATTGTTTCCGAAATCAGAGGAAAGGTCTTAGCCCTGAGTCAACACAAATT TGCCAGCAATGTAGTAGAAAAGTGTGTTACTCATGCCTCCCGTGCTGAGAGAGCTTTACTGATTGATGAG GTCTGCTGTCAGAATGATGGTCCTCACAGTGCCTTATACACCATGATGAAGGATCAGTATGCCAACTATG TGGTTCAGAAGATGATTGATATGGCTGAGCCTGCGCAGAGAAAGATAATCATGCACAAGATTCGACCTCA TATTACTACTCTTCGCAAATACACATATGGGAAGCATATACTGGCCAAGTTGGAAAAATACTATCTGAAA AACAGCCCAGATCTAGGGCCAATTGGAGGACCACCAAATGGGATGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_030723
Insert Size	3201 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030723.2, NP_109648.2
RefSeq Size	6235 bp
RefSeq ORF	3201 bp
Locus ID	80913
UniProt ID	Q80U58
Gene Summary	Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3' UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3' UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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