Nlrp1b (NM_001040696) Mouse Untagged Clone
CAT＃: MC223845
Nlrp1b (untagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1, (10ug)
CNY 15110.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | ENSMUSG00000070390; Nalp1b; OTTMUSG00000006087; OTTMUSG00000006089 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223845 representing NM_001040696
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACAATCTCAGCCCAAGAAGAAAAGTCGCACAAAGGTGGCTCAGCATGAGGGTCAACTAAATTTGA ACCCCACCTTCAAAACAAGGAAACGTAAAGAGGTGGAGCTGATGAAGCGCAGACCCAAGCCAGAGGGACA CTTGAAGCTAGGGACAATTCCAAAAGTACACATAAAACAAAAAGGAGAGACTCTGGACCCCACATGGTCA AGGAAAAGAAAGAATCTGGTCCAAAAGTTAACAAACCTACTACTCTTTGAAAAATTGTGCTCAAGAGGCT CAGAAAACTTGATCAGGAAAAGCTGGTATTCATGTGAAGAAGAAGAAAGAGGACATATGATTGAGATCCA AGACTTATTTGGCCCAAACAGAGGTACCCATAAAAAGCCTCAATTAGTCATAATAGAGGGTGCTGCTGGA ATTGGGAAGTCAACACTGGCCAGGCAGGTGAAGAGAGCCTGGAAGGAAGGCCAGCTCTACAGGAATCACT TCCAGCATGTCTTCTTCCTCAGTTGCAGGGAGCTGGCCCAGTATGAGCAGCTGAGTCTGGCTGAGCTCAT AGCACAAGGCCAGGAAGTGCCCACCGTTCCCATCAGGCAGATCCTGTCTCACCCTAAGGAGCTGCTCTTC ATCCTGGATGGCATAGATGAGCCAGCATGGGTCTTGGCAGACCAGAATCCTGAGCTATGTCTGCACTGGA GTCAAAGACAGCCTGTGCACACTCTGCTGGGCAGTTTACTAGGGAAATCCATCCTTCCTGGGGCTTCCTT CTTGCTCACAGCTCGGACCACAGCTCTACAGAAGATCATTCCTTATGTGGGGCAGCCACGTCGGGTGGAA GTCCTGGGGTTCTCCAAGTTTGAACGGGAGGTCTATTTCCGCAAATATTTTGTGAAGGAGAGCGATGCAA TTGCTGCTTTTAGATTGGTGGTATCTAACCCAGTGCTCCTGACCCTGTGTGAGGTTCCCTGGGTGTGCTG GTTGGTCTGCACTTGCCTGAAAAAGCAGATGGAGCAGGGTGGAGAGCTCTCCCTGACCTCACAGACCACC ACAGCCCTCTGCCTGAAATACCTTTCCCTGACAATCCCAGGGCAGCACATGAGGACCCAGCTCAGAGCCC TCTGCTCACTGGCTGCTGAGGGGATCTGCCAAAGAAGAACCCTGTTCAGTGAAAGCGACCTCTGTAAGCA AGGGTTAGCAGAGGATGCCATTGCCACTTTCCTGAAGATCGGTATCCTTCAAAAGCAGGCCAGCTCTCTG AGCTACAGCTTTGCCCACTTGTGTCTCCAGGAGTTCTTTGCAGTCATGTCTTACATCTTGGAGGATAGTG ATGAAAGATGTGATGGTATGGAATTCAAAAGAACTGTGGAAACACTGATAGAAGTATATGGAAGACATAC TCTGTGTGAGGAACCCACTGTGCACTTTCTATTTGGTCTTGTGAATGAACAGGGAATGAGAGAAATGAAA AAGATCTTTGACTGCAAACTGCCTTTGGGGACAGAGTTGAAGATGCTAAAGAGCACCCTAGGGAACCCAA CCTATCAACATCATCTGGGTTTGCTCCACTGTCTGTATGAAAGTCAGGAAGAGGTGCTCCTGACATATGT GTTATGTAATCTTCACTTAACAGGGCCTGACAAAAATTACATGGAAGCCACAGTGTCACAGACAAATGTG AAGCACCTGGTGATACAGACAGATATGGAGCTCATGGTGGTCACTTTCTGCATTCAGTTCTGTTGTCACG TGAGGAGTCTTCGGGTGAACATGAAAGGACAGCAGGGACATAAGCTGACAGTTGCCAGTATGGTTCTGTA CAGGTGGACCCCAATCACTAATGCCAGTTGGAAGATTCTTTTCTACAATCTTAAATTCAACAGTAATCTG GAGGGGTTGGACCTCAGTGGAAATCCACTGAGTTACTCTGCAGTGCAATATCTCTGTGATGCCATGATAT ACCCTGGCTGCCAACTAAAGACTTTGTGGCTTGTTGAATGCGGCCTCACACCCACATACTGCTCACTCCT GGCCTCAGTGCTCAGTGCCTGCTCCAGCCTGAGAGAGCTAGACCTGCAGCTAAATGACCTGTGTGACGAT GGTGTAAGGATGCTGTGTGAGGGGCTCAGGAATCGTGCCTGCAACCTCAGAATCCTGCGGCTGGACCTGT ACTCTCTCAGTGCCCAGGTGATTACAGAGCTCAGGACACTGGAGGAAAATAATCTGAAGCTGCACATCTC CAGCATCTGGATGCCACAAATGATGGTCCCCACTGAGAACATGGATGAAGAAGATATCCTGACCTCATTC AAGCAGCAGAGACAACAGTCAGGAGCCAATCCCATGGAAATTCTGGGGACTGAAGAAGACTTCTGGGGCC CTATAGGACCTGTGGCTACTGAGGTGGTTTACAGAGAAAGGAACCTGTACCGAGTTCAATTGCCCATGGC TGGTTCCTACCACTGTCCCAGCACAAGACTCCACTTTGTAGTGACAAGGGCAGTGACAATAGAGATCGAA TTCTGTGCCTGGAGCCAATTCCTGGACAAGACTCCCTTGCAGCAGAGTCACATGGTGGTTGGACCTCTGT TTGACATCAAGGCTGAGCAAGGAGCTGTGACTGCAGTGTACCTCCCTCATTTTGTGTCCCTCAAAGACAC AAAGGCAAGCACATTTGACTTCAAGGTGGCCCACTTTCAAGAACATGGGATGGTTCTAGAAACGCCAGAT AGGGTGAAGCCTGGATACACAGTACTGAAAAACCCAAGCTTCTCCCCAATGGGAGTTGTACTGAGAATAA TCCCTGCTGCCCGGCACTTCATCCCCATTACTTCCATCACACTGATCTACTATCGAGTCAATCAGGAAGA AGTCACCCTTCACCTCTACCTAGTCCCTAATGACTGCACCATACAGAAGGCCATAGATGATGAAGAAATG AAGTTTCAGTTTGTGAGAATAAACAAGCCACCCCCAGTAGACAATCTTTTCATTGGCTCCCGCTACATTG TGTCTGGTTCTGAGAACCTGGAAATCACCCCAAAGGAACTGGAGCTGTGCTACAGGAGCAGCAAGGAATT CCAACTCTTCTCTGAGATATACGTTGGAAACATGGGTTCAGAGATTAAGCTGCAAATCAAAAACAAAAAG CACATGAAACTCATATGGGAAGCCTTGCTGAAACCAGGAGACCTCAGACCTGCTCTGCCCAGGATTGCCC AAGCCCTCAAAGATGCCCCTTCCTTGCTGCACTTCATGGACCAGCATCGGGAGCAGCTGGTAGCCCGAGT GACATCAGTGGACCCTCTCTTGGACAAGCTGCATGGTCTGGTGCTGAATGAAGAATCCTATGAGGCAGTG CGGGCTGAGAACACAAACCAAGATAAGATGAGGAAGCTCTTCAACCTCAGCCGGTCCTGGAGTCGAGCCT GCAAAGACCTATTCTACCAAGCTCTGAAGGAGACCCATCCTCACCTGGTCATGGACCTCTTAGAAAAGTC AGGTGGGGTCTCTTTGGGATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001040696 |
| Insert Size | 3525 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040696.1, NP_001035786.1 |
| RefSeq Size | 4159 bp |
| RefSeq ORF | 3525 bp |
| Locus ID | 637515 |
| UniProt ID | A1Z198 |
| Gene Summary | As the sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (By similarity). May be activated by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, in a NOD2-dependent manner (PubMed:18511561). Might be activated by Toxoplasma gondii, although at a lower extent than allele 1 (PubMed:24218483). Contrary to Nlrp1b allele 1, allele 2 is not activated by Bacillus anthracis lethal toxin (PubMed:16429160, PubMed:21170303, PubMed:24492532).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG215179 | Nlrp1b (tGFP-tagged) - Mouse NLR family pyrin domain containing 1B (Nlrp1b) transcript variant 1, (10ug) |
CNY 11400.00 |
|
| MR215179 | Nlrp1b (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1 |
CNY 8176.00 |
|
| MR215179L3 | Lenti ORF clone of Nlrp1b (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1 |
CNY 12260.00 |
|
| MR215179L4 | Lenti ORF clone of Nlrp1b (mGFP-tagged) - Mouse NLR family, pyrin domain containing 1B (Nlrp1b), transcript variant 1 |
CNY 12260.00 |
