Eif5b (NM_198303) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A030003E17Rik; BC018347; IF2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223983 representing NM_198303 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGAAACAGAAAAACAAGAGCGAAGACAGTACCAAGGATGATACTGATTTGGGCGCCTTGGCTG CAGAAATAGAAGGAGCTGGTGCTGCCAAGGAGCAGGAGCCTCAGAAGTCAAAGGGAAAGAAGAAAAAGGA GAAAAAGAAACAAGACTTTGATGAGAACGACATTCTGAGAGAGCTGGAGGAGTTGTCTCTGGAAGCCCAA GGCATCAGAGCTGACAGAGACGCCGCTGCAGTCAAGCCTACAGAAAACAATGAAGAAGAATCTGCCTCAA AACAAGATAAAAAGAAGAAGGGACAAAAAGGCAAGAAAACAAGTTTTGATGAGAATGATAGTGAAGAATT GGAAGATAAAGATTCAAAGTCAAAAAAGACTGCCAGACCTAACTCAGAGGCACCCCTTTCTGGGAGTGAG GATGCTGATGATTCTAATAAACTTTCTAAAAAAGGTAAAAAAGCCCAGAAATCCACGAAAAAACGGGACG GGTCAGAAGAGGATGAAGACAATAGCAAAAGAAGTAAAGAACGTTCGAGAGTAAACTCCTCTGGTGAAAG TGGTGGTGAGTCCGATGAATTTTTGCAGTCCAGAAAAGGACAGAAAAAAAATCAGAAGAACAAGTCCGTT CCTACCGTAGACAGTGGGAATGAGGACGATGACTCTTCCTTCAAAATTAAGACGGTGGCCCAGAAGAAGG CAGAAAAGAAAGAGCGCGAGAAGAAAAAGAGAGATGAAGAGAAAGCGAAGTTGCGGAAGATGAAGGAGAA AGAAGAGCTAGAGAAGGGCAAGAAGGAGCAGAGTAAACAGAGGGAGCCTCAGAAGAGACCTGAGGAGGAG GTGTTGACACTCAGAGGGACTCCTGACACAGGAGCTGCCTCGGAAGAGAAAGGAGACACTGCTGCAGCTC TGGAAGACGACAATGAAGGAGACAAAAAGAAAAAAGATAAGAAGAAAAAGAAAACAGAGAAAGATGAGAA AGAAAAAGAGAAGAAGAAAGGCCCTAGTAAATCTACTGTTAAAGCTATCCAGGAAGCTTTGGCTAAGCTC AAAGAGGAGGAGGAGAGACAGAAGAGAGAGGAGGAAGAAAGGATTAAGCGGCTTGAGGAGCTTGAAGCCA AACGAAAAGAAGAGGAACGATTAGAACAAGAAAAAAGAGAAAGGAAAAAGCAAAAAGAAAAAGAAAGAAA AGAACGGTTAAAAAAAGAAGGAAAGCTGTTAACTAAGTCCCAGAGAGAAGCCAGAGCCAGAGCCGAAGTG ACCCTTAGACACCTCCAAGCTCAGGGTGTTGAAGTGCCATCAAAAGACTCGTTGCCAAAGAAAAGGCCAG TTTATGAAGATAAGAAGAAGAAGAAAACACCACAGCAGCTAGAGAGTAAAGAAGTATCTGAAACTTTGGA AATAAGTGCTCCTGTGGAAGCTGTGGACCAAGGAGGACCAGAAAAGGAAGAGACGCCACCTTCTGTTGAG CCAGAGGAAGAGGAAGACACCGAAGATGCTGGCTTAGATGACTGGGAGGCTATGGCCAGTGATGAGGAGA GAGAAAAAGAAGGAAATATGATTCACATAGAAGTAGAAGAAAACCCTGAGGAAGAAGAAGAGGAAGAAGA AGAGGAGGAGGAAGAAGAGAGTGAAGATGAAGAAGAGGAAGGGGACAGTGAAGGCAGCGATGGGGACGAG GAGGACTGCAAGCTGTCGGATGAGAAGGACTCAGGGAAGGCTGGAGACACGAAGCCCAGCAAAGACGCTA GCTCAGACTCCGAGTACGACTCCGACGACGATCGGACTAAAGAAGAGCGGGCGTACGACAAAGCGAAACG GAGGATTGAGAAACGACGACTTGAACATGGTAAAAATGTAAATACAGAAAAGCTGAGAGCCCCTATCATC TGTGTGCTTGGACATGTAGACACAGGAAAGACAAAAATTCTAGATAAGCTCCGTCACACACATGTGCAAG ATGGCGAAGCAGGTGGTATTACACAGCAGATTGGTGCCACAAATGTTCCTCTTGAAGCTATTAATGAACA AACTAAGATGATTAAAAATTTTGACAGAGAGAATGTCCGAATCCCAGGGATGCTGATTATTGATACTCCT GGACACGAGTCTTTCAGTAACCTGAGAAACAGAGGAAGCTCTCTTTGTGACATTGCCATTTTAGTTGTTG ACATTATGCATGGTTTAGAGCCCCAGACAATTGAATCAATCAACATTCTCAAATCTAAAAAGTGCCCCTT CATTGTTGCACTTAATAAGATTGACAGGTTATATGATTGGAAGAAGAGTCCTGATTCTGATGTGGCTGTT ACTTTAAAAAAGCAGAAGAAGAATACAAAGGATGAATTTGAGGAGCGTGCAAAGGCCATTATTGTAGAAT TTGCACAGCAGGGTTTGAATGCTGCTTTGTTTTATGAGAATAAAGATCCCCGCACTTTCGTGTCCTTGGT ACCTACCTCTGCACATACTGGTGATGGCATGGGAAGTCTCATCTACCTTCTTGTGGAGTTAACTCAAACC ATGTTGAGCAAGAGGCTTGCACACTGTGAAGAGCTGAGAGCACAAGTCATGGAGGTTAAAGCTCTCCCAG GAATGGGCACCACAATAGATGTCATATTGATCAATGGACGTTTGAAGGAAGGGGATACAATCATTGTCCC TGGAGTAGAAGGGCCCATTGTCACTCAGATTCGAGGCCTCCTTTTACCTCCTCCTATGAAGGAATTACGT GTGAAGAACCAATATGAAAAGCATAAAGAAGTGGAAGCGGCCCAGGGAGTAAAGATTCTTGGGAAAGACC TTGAAAAAACCCTGGCTGGCTTACCTCTCCTAGTGGCCTACAAGGATGATGAAATCCCAGTTCTCAAAGA TGAATTGATCCATGAGTTAAAGCAGACACTAAATGCTATCAAACTAGAAGAAAAAGGAGTTTATGTCCAG GCATCTACACTGGGCTCTTTGGAAGCTCTTCTTGAATTTCTGAAAACATCAGAGGTGCCATATGCAGGAA TTAACATTGGCCCAGTCCATAAAAAGGACGTTATGAAGGCTTCAGTGATGTTGGAACATGATCCCCAGTA TGCAGTAATTTTGGCCTTTGATGTAAGAATTGAACGGGACGCACAAGAGATGGCTGATAGTTTGGGAGTT AGAATCTTTAGTGCAGAAATTATTTATCACTTATTTGATGCCTTTACAAAATACAGACAGGACTACAAAA AACAAAAACAAGAAGAATTTAAGCACATAGCAGTATTTCCCTGCAAGATGAAAATCCTCCCTCAATACAT TTTCAATTCTCGAGATCCAATAGTAATAGGGGTCACTGTGGAGGCTGGTCAAGTGAAACAAGGAACACCT ATGTGCGTTCCAAGCAAAAATTTTGTTGACATTGGGATAGTAACAAGCATTGAAATAAACCACAAGCAGG TGGATGTTGCAAAAAAAGGACAAGAGGTCTGTGTCAAAATAGAACCTATCCCTGGGGAATCTCCCAAAAT GTTTGGGAGACATTTTGAAGCTACAGATATACTTGTCAGCAAGATCAGCCGGCAGTCCATCGATGCACTC AAAGACTGGTTCAGAGACGAAATGCAGAAGAGTGACTGGCAGCTTATTGTGGAGCTGAAGAAAGTATTTG AAATCATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_198303
Insert Size	3651 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_198303.2, NP_938045.2
RefSeq Size	7797 bp
RefSeq ORF	3651 bp
Locus ID	226982
UniProt ID	Q05D44
Gene Summary	Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.[UniProtKB/Swiss-Prot Function]

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