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Rptor (NM_028898) Mouse Untagged Clone
CAT＃: MC224267
Rptor (untagged) - Mouse regulatory associated protein of MTOR, complex 1 (Rptor), (10ug)
CNY 13,712.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4932417H02Rik; mKIAA1303; r; Rap; Raptor |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224267 representing NM_028898
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGGAGATGCTGCAGTCGCCTCTTATGGGACTCGGGGAGGAAGATGAGGCGGACCTTACAGATT GGAACTTGCCTTTGGCTTTTATGAAGAAAAGACATTGTGAGAAAATCGAAGGCTCCAAATCTTTAGCGCA GAGCTGGAGAATGAAGGATCGGATGAAGACGGTGAGTGTGGCCTTGGTCCTGTGCCTGAATGTGGGTGTG GATCCTCCGGATGTGGTGAAGACCACACCCTGTGCTCGGCTGGAATGCTGGATCGATCCTCTGTCTATGG GCCCTCAGAAAGCTCTGGAAACCATCGGTGCAAACCTACAGAAGCAGTATGAGAACTGGCAGCCAAGGGC TCGGTACAAGCAGAGCCTCGACCCTACTGTGGATGAAGTCAAGAAACTTTGCACATCTCTGCGCCGGAAC GCCAAGGAGGAACGGGTCCTTTTCCACTACAATGGCCACGGGGTGCCCAGGCCTACAGTGAATGGAGAGG TCTGGGTCTTCAACAAGAACTACACTCAGTACATCCCTCTGTCCATATACGACCTGCAGACGTGGATGGG CAGCCCATCCATCTTTGTCTACGACTGTTCCAATGCTGGCCTCATCGTCAAGTCCTTCAAACAGTTTGCA CTGCAGAGGGAGCAGGAACTAGAGGTAGCTGCGATTAACCCAAACCATCCACTTGCCCAGATGCCCTTGC CTCCCTCAATGAAAAACTGCATCCAGTTGGCAGCGTGTGAGGCACACGAGCTGCTGCCCATGATCCCTGA CCTCCCAGCCGACCTGTTCACATCCTGCCTCACCACTCCCATCAAGATTGCTCTGCGCTGGTTTTGCATG CAGAAGTGCGTGAGTCTGGTGCCTGGAGTCACACTGGATTTGATAGAAAAGATCCCTGGCCGGCTGAATG ACCGGAGGACCCCTCTGGGAGAGCTGAACTGGATCTTCACAGCCATCACAGATACCATCGCCTGGAATGT GCTCCCTCGGGATCTCTTCCAAAAGCTTTTCCGACAGGACCTGCTCGTGGCAAGTTTGTTTAGAAATTTT TTATTGGCAGAAAGAATCATGAGGTCATATAACTGCACCCCAGTAAGCAGCCCGCGGCTCCCCCCGACAT ACATGCATGCCATGTGGCAGGCCTGGGACCTTGCTGTGGACATTTGCCTCTCTCAGCTGCCAACCATCAT CGAAGAGGGTACTGCCTTCAGGCACAGCCCATTCTTTGCCGAGCAGCTGACAGCATTCCAGGTGTGGCTC ACAATGGGTGTGGAAAACAGGAGCCCCCCAGAGCAGCTGCCCATCGTCCTGCAGGTGCTGCTAAGCCAGG TGCACCGGCTGAGAGCATTGGACTTGCTGGGACGGTTTTTGGACTTGGGCCCTTGGGCGGTGAGCTTGGC CCTGTCGGTGGGCATCTTCCCCTATGTACTGAAGCTGCTTCAGAGCTCAGCCCGAGAGCTTCGGCCACTC CTTGTCTTCATCTGGGCCAAAATCCTCGCTGTCGACAGTTCGTGCCAAGCTGACCTCGTGAAGGACAACG GTCACAAGTACTTCCTATCCGTCTTGGCAGACCCATACATGCCAGCTGAACATAGGACCATGACAGCCTT CATTCTTGCTGTAATCGTCAACAGCTACACAACAGGGCAAGAGGCCTGCCTCCAGGGAAACCTGATTGCC ATCTGTCTGGAGCAGCTCAGCGACCCACACCCCTTGCTACGCCAGTGGGTGGCCATTTGCCTGGGAAGGA TCTGGCAGAACTTCGACTCTGCAAGATGGTGTGGGGTGAGAGACAGTGCCCACGAAAAGCTCTATAGCCT CCTCTCTGACCCCATCCCTGAGGTCCGATGTGCAGCTGTATTTGCCCTGGGCACCTTTGTGGGAAATTCT GCTGAGAGGACAGACCACTCTACCACCATTGACCACAATGTGGCCATGATGCTGGCTCAGCTGATCAATG ATGGAAGCCCCATGGTCCGGAAGGAGCTGGTGGTGGCTCTGAGTCACCTTGTAGTCCAATATGAAAGCAA TTTCTGCACTGTGGCCCTGCAGTTCATGGAGGAAGAAAAGAACTACCCTTTGCCTTCTCCAGCAGCCACA GAGGGAGGGAGCTTGACCCCAGTTCGAGACAGCCCATGCACCCCCAGACTCCGTTCAGTGAGCTCCTATG GGAACATCCGAGCCGTCACGACAGCTAGGAACCTGAACAAATCTTTGCAGAATCTGAGCTTGACGGAAGA ATCGGGCAGCTCAGTGGCCTTCTCTCCCGGGAACCTCAGCACCAGCAGCAGTGCCAGCAGCACCTTGGGG AGCCCTGAGAACGAGGAGTACATCCTGTCCTTCGAGACCATCGACAAGATGCGGCGTGTGAGCTCCTACT CAGCGCTCAACTCCCTCATAGGAGTTTCTTTTAATAGTGTTTACACTCAAATTTGGAGAGTCTTACTGCA TCTGGCTGCTGATCCCTATCCAGATGTTTCTGATTTAGCCATGAAAGTCCTCAACAGCATTGCTTACAAG GCCACAGTGAACGCCCGGCCCCAACGAATCCTTGACACATCCTCTCTCACACAGTCGGCCCCAGCCAGCC CAACCAACAAGGGCATGCACATGCACCAGGTGGGAGGCTCCCCGCCAGCATCTAGCACCAGCAGCTGCAG CCTGACCAATGATGTGGCCAAGCAGACTGTCAGCCGTGACCTGCCTTCCAGCCGCCCGGGTACTGCCGGC CCCACAGGGGCACAGTACACCCCTCACTCCCACCAGTTCCCTCGAACACGAAAGATGTTTGACAAAGGCC CTGATCAGACCACAGATGATGCAGATGATGCTGCCGGACACAAGAGCTTCATTTGTGCCTCTATGCAGAC AGGGTTCTGTGACTGGAGTGCCCGCTACTTTGCCCAGCCAGTCATGAAGATCCCCGAGGAGCATGACCTA GAGAGTCAGATCCGCAAGGAGCGTGAGTGGCGGTTCCTGAGGAACACTCGAGTCAGGAAGCAGGCTCAGC AGGTCATCCAGAAGGGCATCACCAGACTGGACGATCAGATCTTTCTGAACAGGAACCCCGGCGTTCCTTC TGTGGTCAAATTCCATCCCTTTACACCATGCATAGCTGTCGCCGACAAGGACAGCATCTGTTTTTGGGAC TGGGAGAAAGGAGAGAAGCTGGACTATTTCCACAATGGCAACCCTCGGTACACCAGGGTCACCGCCATGG AGTACCTGAATGGTCAGGACTGTTCCTTGCTGCTGACAGCCACAGATGATGGTGCCATCAGGGTCTGGAA GAATTTTGCTGATTTGGAAAAGAATCCAGAGATGGTGACTGCGTGGCAAGGGCTCTCAGACATGCTTCCA ACAACACGAGGAGCTGGGATGGTGGTAGACTGGGAACAAGAAACTGGCCTTCTCATGAGCTCAGGGGACG TGCGCATTGTCAGGATCTGGGATACTGACCGGGAGACGAAGGTGCAGGACATCCCCACGGGTGCTGACAG CTGTGTGACAAGTCTGTCTTGTGATTCGCACCGTTCGCTCATTGTGGCTGGCCTGGGTGATGGCTCGATC CGTGTCTACGACAGGAGGATGGCACTCAGTGAATGCCGGGTCATGACTTACCGAGAGCACACAGCGTGGG TGGTAAAGGCCTACCTGCAGAAGCACCCTGAGGGCCACATCGTGAGTGTGAGTGTCAATGGAGATGTGCG CTTCTTCGACCCTCGGATGCCCGAGTCTGTGAATGTAATGCAGATTGTGAAGGGGCTGACAGCCCTGGAC ATCCACCCCCAGGCAAACCTGATCGCCTGTGGCTCCATGAACCAGTTCACAGCCATCTACAACGGAAATG GAGAGCTGATCAACAACATCAAATACTATGATGGCTTCATGGGCCAGCGAGTCGGGGCCATCAGCTGCCT GGCTTTCCACCCACACTGGCCTCATCTGGCCGTAGGAAGCAATGACTACTACATTTCCGTGTATTCGGTG GAGAAGCGTGTCAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_028898 |
| Insert Size | 4008 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028898.2, NP_083174.2 |
| RefSeq Size | 6956 bp |
| RefSeq ORF | 4008 bp |
| Locus ID | 74370 |
| Gene Summary | This gene encodes a subunit of mammalian target of rapamycin complex 1 (mTORC1), a component of the mTOR signaling pathway, which regulates cell growth in response to nutrient and energy levels. The encoded protein may regulate the assembly, localization, and substrate binding of the mTORC1 complex. Homozygous knockout mice for this gene exhibit embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225740 | Rptor (tGFP-tagged) - Mouse regulatory associated protein of MTOR complex 1 (Rptor), (10ug) |
CNY 14,630.00 |
|
| MR225740 | Rptor (Myc-DDK-tagged) - Mouse regulatory associated protein of MTOR, complex 1 (Rptor) |
CNY 13,704.00 |
|
| MR225740L3 | Lenti ORF clone of Rptor (Myc-DDK-tagged) - Mouse regulatory associated protein of MTOR, complex 1 (Rptor) |
CNY 15,200.00 |
|
| MR225740L4 | Lenti ORF clone of Rptor (mGFP-tagged) - Mouse regulatory associated protein of MTOR, complex 1 (Rptor) |
CNY 15,200.00 |
