Arhgap32 (NM_177379) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3426406O18Rik; Gc-gap; Grit; mKIAA0712; p200Rhogap; p250Gap; Px-rics; Rics
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224898 representing NM_177379 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCTCGCCCAACAAAACAGAAGCTAAAGCAGCGGGGCATCTTGAAAGAGAGAGTATTCGGTTGTG ACTTGGGGGAGCACCTCCTCAATTCTGGGTTTGAAGTGCCCCAGGTTCTTCAGAGCTGCACAGCGTTCAT CGAGAGGTATGGCATTGTGGATGGGATATATCGTCTCTCTGGCGTTGCCTCCAATATCCAGAGACTTCGT CATGAATTTGATTCTGAGCATGTCCCCGACCTGACAAAAGAACCTTATGTTCAAGACATCCATTCTGTGG GCTCCCTCTGTAAGCTGTACTTCCGAGAACTCCCAAACCCTCTGCTCACCTACCAGCTATACGAGAAGTT CTCTGATGCTGTTTCAGCAGCAACGGATGAAGAACGGTTGATAAAAATCCATGATGTCATCCAGCAACTC CCCCCACCACACTATAGAACCCTGGAGTTCCTGATGAGACACCTGTCTCTTCTAGCAGACTATTGCTCCA TCACAAATATGCATGCGAAAAACCTTGCCATTGTGTGGGCACCAAACCTGCTGAGATCAAAGCAGATAGA GTCAGCTTGCTTCAGTGGGACAGCAGCTTTCATGGAAGTGAGGATTCAGTCTGTGGTTGTTGAATTCATT CTCAATCATGTGGATGTGCTCTTCAGTGGCAAAATCAGCGCTGTCATGCAGGAAGGAGCAGCTTCTCTGT CAAGGCCCAAGTCCCTGCTGGTCTCGTCTCCATCTACCAAGCTGCTGACATTGGAGGAAGCCCAGGCAAG GACGCAGGCTCAGGTCAGTTCTCCTATTGTGACGGAAAATAAGTACATCGAAGTCGGAGAAGGGCCTGCT GCGCTGCAAGGGAAGTTTCACACCGTCATTGAGTTCCCACTTGAAAGAAAGAGGCCTCAGAATAAGATGA AAAAATCTCCCGTTGGCAGCTGGCGTTCCTTTTTCAATTTGGGGAAGTCCTCTTCTGTCTCCAAACGCAA GTTGCAGCGTAATGAAAGTGAGCCCTCAGAAATGAAAGCCATGGCTTTGAAAGGTGGCAGGGCAGAAGGA ACCCTCCGCTCAGCTAAAAGTGAAGAGTCCCTTACTTCTCTCCATGCAGTTGACGGTGATTCCAAGTTGT TCCGACCTAGAAGACCCAGATCCAGCAGCGATGCGCTCTCTGCCTCTTTCAATGGCGACGTGCTGGGGAA CCGGTGCAATTCCTATGACAATCTGCCCCACGACAACGAGAGTGAAGAGGAAGTGGGGCTGCTCCACATT CCAGCTCTTGTGTCTCCTCATTCGGCTGAGGATGTTGACTTGAGTCCACCAGACATCGGAGTGGCCAGTC TGGACTTTGATCCCATGTCATTTCAATGTAGTCCTCCAAAAGCTGAGTCAGAGTGTCTGGAGAGTGGTGC TTCCTTTCTGGATTCATTAGGTTATACCAGGGATAAACTGAGTCCCAGTAAGAAGGATGCAGAAGCAGGT GGTAGCCAGTCACAGACTCCAGGAAGCACCGCAAGTTCCGAACCTGTATCTCCAGTCCAGGAGAAACTGA GTCCGTTTTTCACCCTGGACTTGAGCCCCACTGACGACAAGTCTTCTAAGCCATCTTCATTTACAGAAAA GGTTGTTTATGCTTTCTCTCCGAAGATTGGGCGGAAGCTAAGCAAATCTCCTTCCATGAACATATCTGAG CCTATTTCAGTGACTCTCCCACCTCGGGTATCAGAAGTCATCGGTACGGTCTCCAATACTGTAGCTCAGA ATGCATCACCTACATCTTGGGACAAAAGTGTGGAAGAAAGAGATGTCATAAATAGATCCCCCACCCAGCT GCAGCTAGGAAAGATGAAAGCAGGTGAGAGAGAGGCACAGGAGACCTGTGAACCAGAAGCACAGCCCCTG GAACAGGGGGCAGCTGAAGAAGTAGAGTTGCCAGGGACAGAGGAGCGGCCTGTCCTGAGCAGTCAGAGCA AGGCTGTACCGTCTGGACAGAGTCAGACAGGAGCAGTTACCCATGACCCCCCTCAGGATCCCGTTCCTGT CAGTTCAGTCTCTCTTATCCCACCACCACCGCCTCCGAAAAATGTCGCCCGAATGTTGGCACTAGCGTTA GCTGAGTCTGCACAGCAAGCCTCAAGTCAGACACTGAAGAGACCAGGGGCTTCCCAGGCTGGGTGCACTA GTTATGGAGACACGGCAGTCGTTCCATCTGAAGAGAAGCTGCCTAGTTCCTACTCTAGCCTTACTCTAGA TAAAACCTGTTTCCAAACGGACCGGCCAGCAGAGCAGTTCCACCCGCAGATCAATGGACTCGGAAACTGT AACCAGCCTCTCCCTGAGGCAGCAGCTATGGGAGGTCCCACCCAGTCCAACACAACTGACTCTGGGGAGC AACTCCACCAAGTAGACTTAATAGGGAATTCGCTACATCGAAACCATATATCTGGGGATCCAGAAAAGGC TAGAAGCACTTCAGCTCCCTTAACAGACTCAGAGAAGTCTGATGATCATGGAAGTTTCCCTGAAGACCAC GCTGGGAAGAGCAGCGTGTCCACTGTCTCCTTTTTGGAGCAGGACCAGTCTCCACTCCATTTCTCCTGTG GAGATCAGCCCCTCTCTTACCTTGGTACCAGTGTGGATAAACCCCACCATTCCTCAGAACTTACAGACAA ATCTCCCATGCCTTCTACTTTGCCTAGGGACAAAGCCCACCACCCTCTCTCTGGGTCCCCTGAAGAGAAT TCCAGCACAGCCACCATGGCTTACATGATGGCAACTCCAGCAAGAGCGGAACCGAGCAACAGCGAGGCCA GCAGGGTCCTGGCTGAGCAGCCCTCGGCTGCTGATTTTGTGGCTGCCACCCTTCAGCGCACGCACAGAAC TAATCGGCCCCTCCCTCCACCTCCTTCCCAGAGGCCTGCAGAGCAGCCACCAGTTGTGGGGCAGGTACAA GAAGCACCAAGTATAGGATTAAATAATTCCCACAAGGTCCAGGGAACGGCTCCAGCTCCCGAGAGGCCAC CTGAGTCTCGGGCCATGGGTGACCCCGCACCCATCTTCCTCAGTGATGGCACTGCTGCTGCCCAGTGTCC CATGGGCGCCTCTGCTCCTCAGCCTGGACTGCCTGAGAAGGTTCGGGAAAGCAGCAGGGCTCCACCACTC CACTTACGTGCTGAGTCTTTTCCTGGCCATTCCTGTGGCTTTGCTGCCCCAGTTCCCCCAACACGGACAA TGGAGAGCAAGATGGCTGCTGCCTTGCACTCCAGCGCTGCAGATGCCACCAGCAGTTCAAATTACCACTC CTTTGTCCCTTCGTCAGCCTCAGTGGATGATGTGATGCCCGTGCCACTCCCCGTCTCACAGCCTAAGCAT GCTTCTCAGAAGATAGCCTATTCCTCCTTTGCTAGGCCTGATGTCACCGCAGAGCCCTTTGGTCCAGAAA ACTGTTTGCATTTCAATATGACTCCAAACTGCCAGTTTCGCCCCCAGAGTGTTCCTCCACACCACAATAA GTTGGAGCCACATCAGGTATATGGTGCCCGGTCAGAGCCACCAGCCTCCATGGGTCCTCGTTATAACACC TATGTGGCGCCGGGAAGAAACATGTCTGGACACCACTCCAAGCCGTGTAGTCGGGTTGAGTATGTCTCTT CTCTGGGCTCCTCGGTTAGGAATCCTTGTTGCCCTGAAGACATTCTACCTTACCCTACCATCCGGAGGGT ACAGTCCCTCCATGCACCCCCACCTTCCATGATCCGCTCTGTTCCCATTTCACGGACAGAAGTTCCCCCA GATGATGAACCAGCCTACTGCCCAAGACCAGTCTACCAGTATAAGCCATACCAGTCCTCCCAGGCCCGCT CAGACTATCACGTAACTCAGCTTCAGCCTTACTTTGAGAATGGACGGGTCCATTATCGCTATAGTCCGTA CTCCAGCTCCTCCAGCTCCTATTACAGTCCTGAGGGGGCCTTGTGTGATGTCGATGCCTATGGCACAGTC CAGCTGCGGCCCCTCCACCGCCTGTCCAGTCGGGACTTTGCTTTCTACAATCCAAGGCTACAAGGAAAGA ACGTGTACAACTATGCTGGTTTGCCTCCACGCCCACGGGCCAATGCAACTGGCTATTTCTCTGGTAATGA CCATAATGTAGTCACTATGCCCCCCACTGCTGACGGAAAGCACACCTACACCTCATGGGACCTTGAGGAC ATGGAAAAATACCGCATGCAGTCCATCCGCCGAGAGAGCCGAGCACGGCAGAAGGTAAAAGGGCCTATCA TGTCGCAGTATGACAACATGACACCAGCTGTGCAAGAAGACTTGGGAGGGATCTATGTCATCCATCTGCG CAGCAAATCAGATCCTGGGAAAACTGGACTTCTCTCTGTGGCAGAGGGGAAAGAGGGACGGCACCCAGCC AAGGCTGTCAGTCCTGAGGGGGACGAGCGTTTCTACAGGAAGCACCCAGAGTCCGAATTTGACAGAGCCC ACCATCATGGAGGGTATGGCAGCACACAGGCAGAAAAGCCATCCCTCCCGCAGAAGCAAAGCAGCCTTAG GAACAGAAAGCTTCACGATATGGGTTGTAGCCTCCCAGAGCACAGGGCGCATCAGGAAGCAAGCCATAGG CAATTATGTGAATCAAAAAATGGACCACCTTATCCCCAGGGAGCTGGCCAGTTAGATTATGGGTCCAAAG GGATGCCAGACACTTCTGAGCCAAGCAACTACCATAACTCTGGGAAATATATGACATCAGGGCAGGGGTC TCTAACCCTGAACCACAAGGAAGTGAGGCTTCCCAAAGATCTGGACAGACCTCGAGCCAGGCAGCCACCT GGCCCTGAGAAACACTCCAGAGACTGCTACAAGGAGGAGGAACACTTCTCTCAGTCTATGGTCCCACCCC CCAAACCAGAGAGGAGTCACAGCCTAAAACTTCACCATACCCAGAACCTGGAGAGGGACCCCAGCGTGCT ATACCAGTATCAGACACACAGCAAGCGCCAGAGCAGCATGACTGTTGTGTCCCAGTATGATAACCTGGAG GATTACCACTCCCTGCCCCAGCACCAGCGAGGAGGCTTTGGAGGGGCGGGCATGGGGGCCTATGTGCCCT CTGGCTTTGTCCATCCACAGAGCAGGACATACGCCACAGCATTGGGTCAGGGGGCCTTCCTGCCCACAGA GTTGTCCTTGCCACATCCCGACACGCAGATCCATGCAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177379
Insert Size	5223 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177379.4, NP_796353.3
RefSeq Size	9969 bp
RefSeq ORF	5223 bp
Locus ID	330914
UniProt ID	Q811P8
Gene Summary	GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translational start codon, compared to variant 1. The encoded isoform (2, also known as RICS) is shorter at the N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.