Trip11 (NM_028446) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610511G22Rik; 3110031G15Rik; 6030460N08Rik; AI450776; GMAP-210; TRIP230
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225100 representing NM_028446 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCTGGCTCGGGGGCCTCGGCTCCGGCTTGGGCCAGTCTCTCGGTCAAGTAGGGGGCAGCCTGG CTTCCCTCACTGGCCAGATTTCGAACTTCACGAAGGATATGCTGTTGGAGGGCACGGAGGATGTGCCAGC AGATTTCCCTAATTCCGGGAGAGAGGAGAATGAAGCCACTCATTCAGTCTTAAGATCAGAGAATGAAAGG CTTAAAAAGCTTTACACTGATCTAGAGGAAAAGCATGAAGCATCCGAACTTCAAATTAAGCAGCAATCTT CCAGTTACCGAAGTCAGTTACAGCAGAAAGAGGAAGAAATCAACCACCTTAAAGCAAGGCAGCTCGCACT ACAGGATGAACTGCTCAGACTGCAGTCAGCTGCTCAGTCAGCCCACTTAGGATCTGGCAGCGCACCAGCA GCCAGTGCATCATCTTCATTCAGCTATGGGGTCAGTCATCGTGTGTCAGCCTTCCATGAAGACGACATGG ACTTTGGTGATGTGATCTCATCACAACAAGAAATAAACAGATTATCAAATGAAGTTTCAAGACTTGAGTC TGAACTTGGACATTGGAGACATATTGCTCAGACTAAGGTACAGGGAGCACAAAGCTCTGATCAAACTGAA ATCTGCAAACTACAAAATATTATTAAGGAACTTAAACAGAACAGAAGTCAGGACCTCGATGACCATCAGC ATGAGCTGTCAGCCCTGCAGAACGCTCATCAGCAGAAGCTGACAGAGATAAGTCGGCGGCATCGAGAGGA GTTAAGTGACTATGAAGAGCGTATTGAAGAACTGGAAAATCTGTTACAGCAAGGTGGCTCAGGAGTTACG GTAACTGATCACTCTAAAGTGTATGAGATGCAAAACACTATTCAGATCTTACAGATGGAAAAAGTAGAAT CTACCAAACAAATCGAAGACCTTGAGAATAAAATAAAAGAGATACATAAAAGATTGTCTTCTGCAGAACA TGACCAGGAAGTCTGGAAAAAAGAACAAGAGCGGCTGGAAGTGGAAAAGAGAGAGATGACTGAGCAGTGT GAGCGCTTGAAGCTGGAACTCAGTGAAGCCCAGCAGAGTGCTCTGCGGCAGAGTGATGCTGCGGTGGAGG AGGAGACGATCCTCCCACACAGCTCCTCAGTCGCAGAGGTCCTGAGATTGCAGCAAGCTTTGACTGATGC TGAAAATGAAATAATGAGACTAAGGAGTTTAAACCAGGATATCAGTCTTGCTGAAGACAATCAGAAACTT CAAATGTGTGTCCAAACTTTAGAAAAAGAGAAGTCTTTATTGAGTCAAGAAAAGGAAGAACTTCAGATAT CACTTTCCAAACTGAGCAGTGAATATGAAGTCATTAAAAGTACAGCTACAAGGGACTTAGATTTGTTTTC TCAAGTACATGACTTAAAACATAACCTGGAGGCGAAGGAGCAAGAACTGAACCAGAGTATTCATGAAAAT GAAATCCTTATGGCTGAATTAGAAGAACTGGACAAGCAGAACCAAGAGGCTACTAAGCATGTGATTTTGA TAAAAGATCAGCTATCAAAACAACAAAGTGAAGGAGATAGTGTCATCAAGAAACTGAAAGAAGAGCTAGC TGGTGAGAAGCAGAGAACCCATCAGCTTGAGGATGATAAAATGAATATTATTAAAGAGTTAACCGTACAG AAAGAAAAGCTAACTCACAGTGAACAGGCCCTAAGTGATTTACAGTTAACCAAACAGAAGCTTGAAGATA AAGTAGAAGATTTAGTAGATCAGCTAAGTAAATCAGAGAAAAACAATTTTGACATTCAGAAGGAGAACCA TGAACTGAGGGAACACATCAGACAAAATGAAGAGGAGCTGTCCACAGTCAGGAGTGAGTTAACTCAGTCC CAAACCCAGGGCTCCAGCAGGAACGTGAAGGATGACTTACTTAAAGAAAGGGAAACTCAAGTTCAAAACT TAAAGCAAAATCTTTCAGAAGTAGAACAGCTCAATGAACATTTAGAGCAAGTTGCTTTTGATCTCAGAAC AGAAAATGAAGAGTTGCTTGAAGCATATGAAGAGGTAAGAAATCAGTTGGAAGAATCTGTTGCTGGCAAC AAGCAGATTTCTCTGGAAAAAACTGCTATGCTGGAATGGGAAAAGGCACCACTAGAAACAGAGCTGTGTC GAGCTGAAAAGCGGGTGTTGGAAGAAGAGCGGAAGTACGAGCAAACTGTCCAGGAACTGTCCAGCGCGTG CAGCCCCGATACCTCCGCCTTACAGCTGGAACAGGAGCGCTTAATTCAACTCAATCAAGAAAAAGACTTT GAAATAGCAGAACTCAAAAAAAGTATTGAGCAAATGGATACTGATCATAAACGAACCAAGGAGACTTTGT CATCTAGTTTAGAAGAGCAGAAACAATTGACACAACTTATAAATGAGAAAGAGATTTGTATTGTGAAACT TAAAGAAAAAAGTTCAGAACTGCAGAAGGAATTAGACAAATGTGCTCAGACCTTAAGGAAAAATGAGACT TTAAGGCAGACCATAGAAGAAAAGGATAGAAGTCTTGGATCCATGAAAGAAGAGAACAATCACTTGCAAG AGGAGCTGGAGCGGCTCCGGGAGCAGCAGAGTCGGGTAGTGCCCGCGCCTGAGCCCAGAACCCTTGACAG CACCACCGAGCTGGAGTCTGAGCTGTCTCAGCTGCACAGAATCAAGGGTCATCTGGAAGAAGAGATTAAA CATCACCAGAAGATGATTGAAGATCAAAACCAGAGCAAGCTGCAGCTACTCCAGTCTCTACAGGAGCAGA AGAAAGAACTGGATGAGTTTAAATACCAGCATGAACAAATGAGTATCTCACACACACGACTCTTTTTAGA AAAAGATGAGGAAATTAAGAACTTGCAAAAAACAATTGAACAAATAAAAGCCCAGTTGCATGAAGAAAGG CAGGATAGTCAAACAGAGAATTCCGATATTTTTCAAGAAACAAAAGTTCAGAGCCTTAGTATAGAACATG GAAGTGAAAAACATGACCTCTCTAAAGCGGAGACGGAAAGGTTAGTGAAGGGGATAAAGGAGAGGGAGCT GGAGATCAAGCTTCTGAATGAGAAGAACACCTCGCTAACTAAACAGATCGATCAGCTGTCCAAAGACGAG GTCGGCAAGCTCACGCAGATCATCCAGCAGAAGGACTTGGAGATACAGGCTCTTCATGCCAGGATCTCCT CCGCGTCCTACTCACAGGATGTTGTGTACCTTCAGCAGCAGCTGCATGCATACGCTATGGAAAGAGAGAA AGTGATGGTTATTTTGAATGAGAAGACAAGGGAAAATAGCCAGCTAAAAACAGAATATCATAAGGTGATC GATATTATTAGTGCCAAAGAAGCAGCCCTTATTAAGTTGCAAGATGAGAATAAGAAAATGTCCACTAGAT TTGAAAGTAGTGGTCAAGATATGTTTAAAGAAACTATTCAGAATTTATCCCGGATCATTCGAGAAAAGGA CATTGAGATCGATGCACTGAGTCAGAAGTGCCAGACCCTGCTGACAGTCTTGCAGACTTCTGGCACGGGG AGTGAGGCTGGAGGGGTTAACAGTAACCAGTTTGAGGAGCTCTTACAGGAGCGGGATAAACTGAAGCAGC AGGTGAAGAAAATGGAGGAGTGGAAACAACAAGTGATGACCACCGTCCAGAACATGCAGCACGAGTCTGC CCAGCTCCAGGAAGAGCTTCACCAACTTCAGGCACAAGTTTCGGTTGACAGTGATAACAATTCTAAACTG CAAGTGGACTACACAGGCCTGATCCAAAGTTATGAGCAGAATGAAACCAAACTTAAGAACTTTGGGCAGG AATTGGCACAAGTTCAGCATAGCATAGGGCAGCTTTGTAATACCAAGGACCTTCTTTTAGGAAAACTTGA TATTATGTCTCCTCAGCTCTCCTCTGGCTCATCACTTACCTCTCAGGCAGCAGAGCCTCTTAGAGCAAGC CAGTCTTCTGAGCCACATGAGTCTTCTCAGTTGCTTCAACAAGAAGTAGATGATCTGAGAAAATCACTGC AGGAGAAAGATGCCACAATTCGAACGCTCCAGGAAAACAATCATAGACTGTCTGACTCTGTTGCTGCTTC CTCAGAGGTAGAAAGAAAAGAACACGAGCAAGCTGATTCAGAAATTAAGCAGCTGAAGGAAAAACAAGAG GTTTTACAAAACTTGCTTAAGGAAAAAGACCTCTTAATCAAAGCCAAAAGTGACCAATTACATTCTTCAA ATGAAAACCTTGCTAACAAAGTGAATGAAAACGAGCTTTTGAGGCAGGCAGTGACAAATTTGAAGGAGAG AATATTGATTTTAGAAATGGACATTTCTAAATTAAAAGGGGAAAATGAAAAAATAGTAGACGCATCCAAG GGGAAGGAAACAGAATACCAGGCGTTACAAGAGACCAACATGAAGTTTTCCATGATGTTACGAGAAAAGG AGTTTGAATGCCATTCAATGAGAGAGAAGGCACTTGCTTTTGAGCAACTACTGAAAGAAAAAGAACAGGG CAAGGCTGGGGAGCTAAATCAACTTTTAAATGCAGTTAAATCAATGCAGGAGAAGACAGTTACGTTTCAG CAGGAAAGAGACCAAGTTATGTTGGCCCTGAAACAGAAGCAAATGGAAACCAGTACCCTGCAGAATGAGG TCCAGCGCTTACGGGACAAAGAATCCCGGCTGAACCAGGAGCTACAGAGACTGCGTGACCACCTCTTAGA GTCAGAAGACTCTCATACGCGGGAAGCTTTGGCTGCAGAAGACAGAGAGGCCAAACTGAGGAAGAAGGTC TCGGTGTTGGAGGAAAAGCTGGTGTCATCCTCTAATGCAATGGAAAACGCAAGTCATCAAGCCAGTGTGC AAGTAGAGTCTTTACAAGAACAACTGAACATGGTCTCCAAGCAAAGAGATGAGACTGCCCTGCAACTCTC TGTCTCTCAGGAACAAGTGAAGCAGTATGCTTTGTCACTAGCCAATCTGCAGATGGTTCTTGAACACTTC CAACAAGAGGAAAAAGCTATGTATTCTGCTGAATTAGAAAAGCAAAACCACCTCTTAGCTGAATGGAAGA AAAAGGCAGAGAGTCTGGAAGGAAAAGTGTTATCGCTGCAGGAACGTTTGGATGAAGCAAATGCTGCACT GGATTCAGCATCAAGACTTACAGAACAGTTAGATTTAAAAGAAGAGCAAATTGAAGAACTTAAAAAGCAA AATGAGCTCCACCAAGAGATGCTGGACGACGCGCAGAAGAAACTGATGAGCTTGGTCAACAGCACAGAAG GGAAAGTGGACAAAGTCCTCATGAGAAACCTCTTTATCGGTCATTTCCACACACCTAAACACCAGCGTCA CGAAGTCCTTCGGCTCATGGGGAGCATCCTGGGTGTTAAAAGGGAAGAGATGGAGCAGCTGTTTGCAGAA GATCAAGGAGGTGTTACTCGGTGGATGGCTGGATGGCTGGGAGGAGGATCGAAAAGTGTGCCCAATACAC CTTTGAGACCAAATCAGCAGTCTGTGTTCAATAGTTCTTTTTCAGAACTCTTTGTTAAATTTCTGGAAAC AGAATCTCATCCATCTGTCCCGCCTCCAAAGCTGTCAGTCCATGGCCTGAAGCCTCTAGATTCTCCAGGA AGAAGGAAAGCTGACACAGGCATACCCGAAAGTTTTAGAGATACAGCAGAATCCAGGGCAGGAAGAAGAA CAGATGTGAATCCATTCTTGGCTCCCCGCTCAGCAGCTGTGCCCCTTATTAACCCAGCTGGACATGGACC TGGTGGGCCTGGGCATCTTCTTTTGAAACCCATCTCAGATGTTCTACCCACGTTTACACCTTTGCCAGTG TCACCTGACAACAGTGCAGGTGTTGTATTGAAAGACCTTCTAAAGCAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_028446
Insert Size	5931 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_028446.1, NP_082722.1
RefSeq Size	6448 bp
RefSeq ORF	5931 bp
Locus ID	109181

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