Arhgap32 (NM_001195632) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3426406O18Rik; Gc-gap; Grit; mKIAA0712; p200Rhogap; p250Gap; Px-rics; Rics
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225167 representing NM_001195632 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACAGAAAGTGAGACTAGCTCTTTGGGGGATGACAGTGTGTTTTGGTTGGACTGTGAAGGTGTAA CCCAGCTGACTGATGGTGACGAGGAAGAAAGGGAAGAGAGTTTCAGAAAGATGAAGTCCTCAATACATTC TGAAGAGGATGATTTTGTTCCAGAGCTACATAGAAATGTTCACCCCCGAGAGCGGCCTGATTGGGAGGAA ACACTCAGCGCAATGGCAAGAGGAGCAGATGTCCCTGAGATCCCTGGAGACCTGACTCTTAAGTCCTGTG GCAGTACAGCGAGCACGAAGGTTAAGCATGTGAAGAAGTTACCCTTCACAAAGGGACACTTTCCAAAGAT GGCTGAATGTGCACATTTTCATTATGAGAACGTGGAGTTTGGCAGCATACAGCTCTCACTATCAGAAGAA CAGAATGAAGTAATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATTGCCTGTCAGG GCAAGAGTTGGATTGTTAAAAGAAGTTATGAAGATTTTCGGGTACTCGACAAACATCTTCATCTCTGCAT TTATGACCGCCGATTCTCCCAGCTCACAGAGCTTCCCCGCTCTGACGTCCTGAAGGACAGTCCTGAGTCG GTTACTCAGATGCTTACAGCTTACCTGTCACGCCTTTCAACTATCGCTGGCAACAAGATCAACTGTGGGC CTGCCCTTACCTGGATGGAGATCGACAATAAGGGAAACCATCTTCTAGTTCACGAGGAGTCGTCCATCAA CACTCCTGCTGTCGGTGCTGCCCATGTTATCAAGCGCTACACCGCTCGGGCCCCTGATGAACTGACATTA GAGGTGGGAGACATCGTTTCTGTTATTGACATGCCTCCCAAAGTATTAAGCACATGGTGGAGAGGCAAGC ATGGATTTCAGGTGGGACTCTTCCCTGGTCATTGTGTCGAGTTAATTAACCAGAAAGTTCCTCAGTCAGT AACCAACTCAGTGCCAAAACCAGTGTCTAAAAAGCACGGCAAGCTCATTACTTTCTTGCGAACATTCATG AAGTCTCGCCCAACAAAACAGAAGCTAAAGCAGCGGGGCATCTTGAAAGAGAGAGTATTCGGTTGTGACT TGGGGGAGCACCTCCTCAATTCTGGGTTTGAAGTGCCCCAGGTTCTTCAGAGCTGCACAGCGTTCATCGA GAGGTATGGCATTGTGGATGGGATATATCGTCTCTCTGGCGTTGCCTCCAATATCCAGAGACTTCGTCAT GAATTTGATTCTGAGCATGTCCCCGACCTGACAAAAGAACCTTATGTTCAAGACATCCATTCTGTGGGCT CCCTCTGTAAGCTGTACTTCCGAGAACTCCCAAACCCTCTGCTCACCTACCAGCTATACGAGAAGTTCTC TGATGCTGTTTCAGCAGCAACGGATGAAGAACGGTTGATAAAAATCCATGATGTCATCCAGCAACTCCCC CCACCACACTATAGAACCCTGGAGTTCCTGATGAGACACCTGTCTCTTCTAGCAGACTATTGCTCCATCA CAAATATGCATGCGAAAAACCTTGCCATTGTGTGGGCACCAAACCTGCTGAGATCAAAGCAGATAGAGTC AGCTTGCTTCAGTGGGACAGCAGCTTTCATGGAAGTGAGGATTCAGTCTGTGGTTGTTGAATTCATTCTC AATCATGTGGATGTGCTCTTCAGTGGCAAAATCAGCGCTGTCATGCAGGAAGGAGCAGCTTCTCTGTCAA GGCCCAAGTCCCTGCTGGTCTCGTCTCCATCTACCAAGCTGCTGACATTGGAGGAAGCCCAGGCAAGGAC GCAGGCTCAGGTCAGTTCTCCTATTGTGACGGAAAATAAGTACATCGAAGTCGGAGAAGGGCCTGCTGCG CTGCAAGGGAAGTTTCACACCGTCATTGAGTTCCCACTTGAAAGAAAGAGGCCTCAGAATAAGATGAAAA AATCTCCCGTTGGCAGCTGGCGTTCCTTTTTCAATTTGGGGAAGTCCTCTTCTGTCTCCAAACGCAAGTT GCAGCGTAATGAAAGTGAGCCCTCAGAAATGAAAGCCATGGCTTTGAAAGGTGGCAGGGCAGAAGGAACC CTCCGCTCAGCTAAAAGTGAAGAGTCCCTTACTTCTCTCCATGCAGTTGACGGTGATTCCAAGTTGTTCC GACCTAGAAGACCCAGATCCAGCAGCGATGCGCTCTCTGCCTCTTTCAATGGCGACGTGCTGGGGAACCG GTGCAATTCCTATGACAATCTGCCCCACGACAACGAGAGTGAAGAGGAAGTGGGGCTGCTCCACATTCCA GCTCTTGTGTCTCCTCATTCGGCTGAGGATGTTGACTTGAGTCCACCAGACATCGGAGTGGCCAGTCTGG ACTTTGATCCCATGTCATTTCAATGTAGTCCTCCAAAAGCTGAGTCAGAGTGTCTGGAGAGTGGTGCTTC CTTTCTGGATTCATTAGGTTATACCAGGGATAAACTGAGTCCCAGTAAGAAGGATGCAGAAGCAGGTGGT AGCCAGTCACAGACTCCAGGAAGCACCGCAAGTTCCGAACCTGTATCTCCAGTCCAGGAGAAACTGAGTC CGTTTTTCACCCTGGACTTGAGCCCCACTGACGACAAGTCTTCTAAGCCATCTTCATTTACAGAAAAGGT TGTTTATGCTTTCTCTCCGAAGATTGGGCGGAAGCTAAGCAAATCTCCTTCCATGAACATATCTGAGCCT ATTTCAGTGACTCTCCCACCTCGGGTATCAGAAGTCATCGGTACGGTCTCCAATACTGTAGCTCAGAATG CATCACCTACATCTTGGGACAAAAGTGTGGAAGAAAGAGATGTCATAAATAGATCCCCCACCCAGCTGCA GCTAGGAAAGATGAAAGCAGGTGAGAGAGAGGCACAGGAGACCTGTGAACCAGAAGCACAGCCCCTGGAA CAGGGGGCAGCTGAAGAAGTAGAGTTGCCAGGGACAGAGGAGCGGCCTGTCCTGAGCAGTCAGAGCAAGG CTGTACCGTCTGGACAGAGTCAGACAGGAGCAGTTACCCATGACCCCCCTCAGGATCCCGTTCCTGTCAG TTCAGTCTCTCTTATCCCACCACCACCGCCTCCGAAAAATGTCGCCCGAATGTTGGCACTAGCGTTAGCT GAGTCTGCACAGCAAGCCTCAAGTCAGACACTGAAGAGACCAGGGGCTTCCCAGGCTGGGTGCACTAGTT ATGGAGACACGGCAGTCGTTCCATCTGAAGAGAAGCTGCCTAGTTCCTACTCTAGCCTTACTCTAGATAA AACCTGTTTCCAAACGGACCGGCCAGCAGAGCAGTTCCACCCGCAGATCAATGGACTCGGAAACTGTAAC CAGCCTCTCCCTGAGGCAGCAGCTATGGGAGGTCCCACCCAGTCCAACACAACTGACTCTGGGGAGCAAC TCCACCAAGTAGACTTAATAGGGAATTCGCTACATCGAAACCATATATCTGGGGATCCAGAAAAGGCTAG AAGCACTTCAGCTCCCTTAACAGACTCAGAGAAGTCTGATGATCATGGAAGTTTCCCTGAAGACCACGCT GGGAAGAGCAGCGTGTCCACTGTCTCCTTTTTGGAGCAGGACCAGTCTCCACTCCATTTCTCCTGTGGAG ATCAGCCCCTCTCTTACCTTGGTACCAGTGTGGATAAACCCCACCATTCCTCAGAACTTACAGACAAATC TCCCATGCCTTCTACTTTGCCTAGGGACAAAGCCCACCACCCTCTCTCTGGGTCCCCTGAAGAGAATTCC AGCACAGCCACCATGGCTTACATGATGGCAACTCCAGCAAGAGCGGAACCGAGCAACAGCGAGGCCAGCA GGGTCCTGGCTGAGCAGCCCTCGGCTGCTGATTTTGTGGCTGCCACCCTTCAGCGCACGCACAGAACTAA TCGGCCCCTCCCTCCACCTCCTTCCCAGAGGCCTGCAGAGCAGCCACCAGTTGTGGGGCAGGTACAAGAA GCACCAAGTATAGGATTAAATAATTCCCACAAGGTCCAGGGAACGGCTCCAGCTCCCGAGAGGCCACCTG AGTCTCGGGCCATGGGTGACCCCGCACCCATCTTCCTCAGTGATGGCACTGCTGCTGCCCAGTGTCCCAT GGGCGCCTCTGCTCCTCAGCCTGGACTGCCTGAGAAGGTTCGGGAAAGCAGCAGGGCTCCACCACTCCAC TTACGTGCTGAGTCTTTTCCTGGCCATTCCTGTGGCTTTGCTGCCCCAGTTCCCCCAACACGGACAATGG AGAGCAAGATGGCTGCTGCCTTGCACTCCAGCGCTGCAGATGCCACCAGCAGTTCAAATTACCACTCCTT TGTCCCTTCGTCAGCCTCAGTGGATGATGTGATGCCCGTGCCACTCCCCGTCTCACAGCCTAAGCATGCT TCTCAGAAGATAGCCTATTCCTCCTTTGCTAGGCCTGATGTCACCGCAGAGCCCTTTGGTCCAGAAAACT GTTTGCATTTCAATATGACTCCAAACTGCCAGTTTCGCCCCCAGAGTGTTCCTCCACACCACAATAAGTT GGAGCCACATCAGGTATATGGTGCCCGGTCAGAGCCACCAGCCTCCATGGGTCCTCGTTATAACACCTAT GTGGCGCCGGGAAGAAACATGTCTGGACACCACTCCAAGCCGTGTAGTCGGGTTGAGTATGTCTCTTCTC TGGGCTCCTCGGTTAGGAATCCTTGTTGCCCTGAAGACATTCTACCTTACCCTACCATCCGGAGGGTACA GTCCCTCCATGCACCCCCACCTTCCATGATCCGCTCTGTTCCCATTTCACGGACAGAAGTTCCCCCAGAT GATGAACCAGCCTACTGCCCAAGACCAGTCTACCAGTATAAGCCATACCAGTCCTCCCAGGCCCGCTCAG ACTATCACGTAACTCAGCTTCAGCCTTACTTTGAGAATGGACGGGTCCATTATCGCTATAGTCCGTACTC CAGCTCCTCCAGCTCCTATTACAGTCCTGAGGGGGCCTTGTGTGATGTCGATGCCTATGGCACAGTCCAG CTGCGGCCCCTCCACCGCCTGTCCAGTCGGGACTTTGCTTTCTACAATCCAAGGCTACAAGGAAAGAACG TGTACAACTATGCTGGTTTGCCTCCACGCCCACGGGCCAATGCAACTGGCTATTTCTCTGGTAATGACCA TAATGTAGTCACTATGCCCCCCACTGCTGACGGAAAGCACACCTACACCTCATGGGACCTTGAGGACATG GAAAAATACCGCATGCAGTCCATCCGCCGAGAGAGCCGAGCACGGCAGAAGGTAAAAGGGCCTATCATGT CGCAGTATGACAACATGACACCAGCTGTGCAAGAAGACTTGGGAGGGATCTATGTCATCCATCTGCGCAG CAAATCAGATCCTGGGAAAACTGGACTTCTCTCTGTGGCAGAGGGGAAAGAGGGACGGCACCCAGCCAAG GCTGTCAGTCCTGAGGGGGACGAGCGTTTCTACAGGAAGCACCCAGAGTCCGAATTTGACAGAGCCCACC ATCATGGAGGGTATGGCAGCACACAGGCAGAAAAGCCATCCCTCCCGCAGAAGCAAAGCAGCCTTAGGAA CAGAAAGCTTCACGATATGGGTTGTAGCCTCCCAGAGCACAGGGCGCATCAGGAAGCAAGCCATAGGCAA TTATGTGAATCAAAAAATGGACCACCTTATCCCCAGGGAGCTGGCCAGTTAGATTATGGGTCCAAAGGGA TGCCAGACACTTCTGAGCCAAGCAACTACCATAACTCTGGGAAATATATGACATCAGGGCAGGGGTCTCT AACCCTGAACCACAAGGAAGTGAGGCTTCCCAAAGATCTGGACAGACCTCGAGCCAGGCAGCCACCTGGC CCTGAGAAACACTCCAGAGACTGCTACAAGGAGGAGGAACACTTCTCTCAGTCTATGGTCCCACCCCCCA AACCAGAGAGGAGTCACAGCCTAAAACTTCACCATACCCAGAACCTGGAGAGGGACCCCAGCGTGCTATA CCAGTATCAGACACACAGCAAGCGCCAGAGCAGCATGACTGTTGTGTCCCAGTATGATAACCTGGAGGAT TACCACTCCCTGCCCCAGCACCAGCGAGGAGGCTTTGGAGGGGCGGGCATGGGGGCCTATGTGCCCTCTG GCTTTGTCCATCCACAGAGCAGGACATACGCCACAGCATTGGGTCAGGGGGCCTTCCTGCCCACAGAGTT GTCCTTGCCACATCCCGACACGCAGATCCATGCAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001195632
Insert Size	6270 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001195632.1, NP_001182561.1
RefSeq Size	10633 bp
RefSeq ORF	6270 bp
Locus ID	330914
UniProt ID	Q811P8
Gene Summary	GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1, also known as PX-RICS). This variant lacks full-length transcript support in mouse, with the 5' exon structure being inferred from homologous transcript alignments. Evidence in the literature, including that in PMIDs 17663722, 16716191, 12857875 and 12531901, support the existence of this longer isoform in mouse. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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