Knl1 (NM_029617) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310043D08Rik; 5730505K17Rik; Casc5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225178 representing NM_029617 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGGGTATATTCAGAAGCTAATGAAGAGAATGACAATACACAGAGACCTGTTAGAAGACAGCATT CCTCAATTTTGAAACCCCCAAGGAGTCCTCTTCAGGACCTCAAATGTGGAAATCAAACAAACCAGGAACC TAATCCTCCAAGAAAAAGGAAAAGCTCTCGTCGAGTCAGCTTTGCAGATACTATAAAAGTATTCCAGACA GAGTCTCACATGAAAACAGAGAGAAATTCAGAAATTTCAGGTATGAACACTTTGCTTTGTGCTCCAATTC AGACTCAGATGCAACAAAAGGAGTTCTCAATTACAGACTGCAACCATGAAAGGAAACATGCAAATGATCA GACAGTCATCTTTTCAGATGAAAACCAGATGGATCTGACAGCAAGTCACACTGTAATGATTACCAAAGGT CTTTCAGACTGTACCAAAAATGAAAATTCTACAAAAATAGATACCACGTCCTTTCTGGAGAATTTAAAGC ATCATGCTGCAAATTCAAGAATTAAAAAGGATTTAGCATGTTCCACAGTTTCCTTAAGTCAAAACATTTT CTCAGAAAAGATCAATTCTGACAACTTCATAAAAAGATTGAAGACAGGAAAACATATTTCGTCTTCTACA GAGCTTGATAAGGAAAATGCTGAGATACCTGTTTATTCCAAAGACTCAAACAGTGCCTCTTCAACATATC AAATGCATGCATCTCTTGGTGTAGATGAAAATAGTAGTAATAGGACTAGAATCTTTAGAGAACAAGATGA TGGTATGAATTTGACCCAATGTCATACAGCCTGTATTAAGACTTGGATTCCTCCATCCACGGAGGCCAAA ATAGGAGAATTCAAAGGTGATAAAACAATTTATGGCAATGAGTGTATGGAGTTGACAACTAACTATACGA TACAGGTTTTGTCATCAGAAAATAATTTATCTGAAAGAGAAACTCAAACTCAAAATGGTATGAATGTTAC AACAGTTGATGGAGCTACAGCTCCAGCTCCAGAGAAGAAAACAGCCCTTAAGGATAAACTAAATGCTGCT TTTCAAGGCTCTTTCCCAAACCCTGAAAATAAAATTCATATTATCAAATGTCATCCTATAGAGTCAGAAA CTCATACAGTCACACAGATTTCTAGTCAAAGTGCCAGCACATTGGCTGTAACTTCAAAATCTATATGTTC TAGTCCAGCTATTGAGGGGTATAAGACAATTTTTCATTCTAGTAGCAATGATGCAATGGAACTGACTAAG TGTCTATCAGCTATGGAAGAGGAAAAAAAATTGCTGAAGGCTGATGATAAGTATTCTAAAATATGTACCA ATCCAGATGCTGGCCCACTCAGAGAGAAAACTATTTATTTAGAAGAGGATAGCATGGACATTACCAAAAG TCACACCGTTGCAATAGATAATAAAATATTTAAACATGATCAAGAAAACATTAAAAAAGAAATAATAGCC ATACCAATATTTGAAAAGGAAATGGTGCTCCGAAATCTTATGCCTATGTCAAAAGATGAGAAAAGGGATG TAAATTATATTTCAGTTCCTCAAGTATCTAAGGAAAGCTTACAACGGAGTCAGACAAACACTTTATCTGT TTCATTGACTGACAAAAAGATGGAATTCTTGGCAGATGAAGATATGGATCTGACTAAAAGTCACACAACT AAGTTAAGTCAAGTTATTCCTACAACTTTTGACTTAGCATCAAAGAATGTCACCAAATCATACTCTCACA GCAAAAGCCCTTTAAATGAGTGGGAAAGCCTGGATAAACAAGTAGTGCTAGGCCAGCATTCTAAACTTCC TTTACCACAGAGGAAAGATAGAGATGATCCTGACTGTTCTCATCATAAGATAATGTATTCAGAGGAGTTA CAAACTATGGATCTAACCAAGAGCCACACTATTGTAATTGGCTTTGGTCCTTCTGAAGTACAAGAACATA GTAAAATTAATTTAGAACACAAAAACAGCCAACTTACAGCAGAAAGCATACAAACAGCTGTGAACGTTCC TGCAGCAAACTCTAGAGTAGTAACAACTAATGATATGGACATGTTAAAGGACAGAAGTACACATAAGCCA GAATTGCTGAAGGAAAAGCAAAACATCAAAATCTATGGAAGAAAAAGTATTGGTAGACTGAAAATTGATA AAACTATATTATTTTCAGAGGGTAATGAGGGTGATATGGATATCACCAAGAGTTGCACAGTCAAAATAAA TCATAGATCTTTATTAGATAAGCATGATTCTCATTTAGTATCTTTGGCAGGAACTTCTAAAACCATTTTG CATGCACGTGGTCAGGTTGAAATGGAAATCAATAGAAGTCACACAACTCCTTTAGAATGTAAAATTATCT CGCCCAGTGATATAACTCCTGGTGATCTGGATAAGACTATGATGTCTATAGATGATCATGAGGAACTAGA CATGACCAAGTCCCATACTGTTTTCATTGACTACCAAGCAGAGGCAAAAGGTGTACTTCCAGACAGACTT GACTTTCAATTATCCAAAAAGGAAAGCCTACAGAAGCCGAAAGTGACATCTCTGGCTGAAGAGATTTACA TTTCAAAAAATAGTGAAAGCAACCATCTACCAGCAAAAGGCAGCCAGCTAACAATATTAGAGGAAGGGTC TAATAGCGGGCTTGGAGAGGAAACTAATGATGCACAAAAGCCTGGATTTCTGAATGAACTTCTGTCAGGC AAAACTCAGAGAAGAAAAAGCCTTAGCCTCAAAAACAAGAGTATTACATTTCCAGAGAATGATAAAAGTT ATAGAGAGATCCCTCAGAGTTCTGCAGTAGAAATAAATAATGAAACTAGGCTGGAAGACAGAAAGGGCTT CAGTTTTGTGCCATTGGCAGGAACTTCCAAACCAGTTTTGTCTGCATATGGGCCCGAGGACATGGAGATT TCTATAGGTCAAACCACTGCTTCAGAATATAAAACTGTCCCACCAGAAGAAATAACTACTATACCTATGG ATAAAACTGTAATGTTTGTAGATAATTTCGGTGATCTGGATGTCACCAGATCCCATACGGTTTTCATCGA TTGTCAAGCCAAAGAAAAGGTACTTGATGAATATACTAACTTGGGAATACAGAAAACAAAAACTTTGAGT GGTTCTGAAGGTGATACCCATATTCAAGAAATCACAAAAAACCCAGCAGCTCAACACAAACACCATATGA CAACTGTTATTCCTTCTAGTACAGTAGTTAGTGATCAAAGTTCAATGAAAATCAAGTTTCACAAAGCTGA TAGAGATGAGGAGGTCAAAGGAAAGGAAGTGGAAGCCAATATGCTGAAACAAACTAAACCTGAAAGCTGT CTGTTAAATATAACAGATGGAAAAAATGTGGATTTTACAAGCAGTTATACAGCTGATGTTTGTAGATCCA GTGACAAGTATTCCTCTTTACCAAATATTTCTTCTTCTGATAATTCGGGTGGTAATACTATGTCCTTGTG TGATAAAAATAAGGAGAAAGCTTATAATTGCCAAGTGCCAAATGAATTTACTTATGCAGCTATCTTACCA AGTACATATCACATGGATTCTAAAAAACTGTCTGTCTTTCCACCTTGTCCTTCAAAAGAAGTCACTCAAA CTGAAAGTGCCATAGCGCTGCTTAAAGATGAAGATCCGGTAGAAGAGCCTCTGGGGGAGATGGCTACATT TAACTCAAAGCATGTATCTCTTAACCTGGCAAAGGATCAAACAGAGGCCTTTGTTGATGTTAGTGTTGCT TCTCAGCCTCATCTCTCAGCCCAACAAAGTCCATCAACTCAAAAAGGGCAGGATGTTGCCCGTAGAGATG AAGGAATATTAGCTAAAGCTGGGAAGAAGGCTTTACCTTTTCTTCTAGAAAATGTCGCTGCATCCACATG GGAAAATGAGTCCAAAATACCCACTAATGTGGAACACTTTGCTGTAACCTATGAAAAAGAACTGAGTATA AGTATTCAAACAGATAAATGTAATACAAATGTACAGAGCCCTAGTAACTCGGCCCTGACAACACAAGTCA TTCAGACGCATGCAAATGCTGAAGGAGCACTAGACTTTTTAGTTCCATCTACTGTTTCATGTTTTAGTAG TACCAAACCAAGTCTGAGTAATTTGAATAGAAAAACTGAGGAGGTCTTAGACTTTCAGACTGTTAATTTA TTACCTCCTGCCGAACAGTTACTTGAAGAAGGAAGTCAGGCACATAGTATGAGTATAGTACAAGCTACAG AAATATATAGACTAGGTTCCAGGAATGATAGAGATGAAGAAAGTAAAACTTTCTGTAATGAGGCTGAAAC TACATCTGTACCACTAAAAACAGCTGTTAAAGACAAAACAAGAAGATGTTCATTGGGGATATTTTTACCC AAATTACCAAGCAAGAGAAGTTGTAGTATCACTGGTGTTGATGACCTGGAACAGATTCTAGCAGATGCAG CTGACTTAACTCAACTAGAAACTCAGCCAGTCTGTAGTAAAGATCCAGGCATTGGATCTGTTGCAGCCAA ACTGAATTTGAGTCCATCTCAGTTTATAAATGAGGAAAATCTCCCTGTATATCCTGGTGAGATACTTTCA TCAGATTCTGTTAGCCTGGACATTGAGGAAAGTGTTCTGATTGACACATCTCAGAGAGAGAGTTTACCGT CTGAAAACAAAACCGAAAACTGCAGGGCCCAAAAAAGAACCCGGGTTGAAGAAAATGATGTTACAAATGA GAAAAAAATCAGAACACATGACAGTGCACAAGATCAAGAGATTTTTGATAATCATATGGAAGAGGATATC AATAAAAATGTCAACAGTGTATTGCTGAAAAGCCTGAGCAGGACACCGTCTAGTTGCAGCAGCTCTCTGG ATTCAATCAAGTCTGATGGACTCTCTCTGGATGTCAGCACACAGCGTAATAGCCAAATGGAATCACAGTT TCTTGGTGACACTATCTCTGAAGAGAGTTTGAAGGAGAAACTCAAGGATGGACAAATTACAATAAAGGAG TTCTTCATACTTCTTCAGGTCCACATCTTAATACAGAAACCTAGGCAGAGCACTCTCCCAGCCAAATTTA CTATAAACACATTACCTACCACAGAAGATCTGATGTTGAGACAATATGTTTATGGACCCAGGATACAGAT TTATAAAGAAGACTGTGAGCTTCTTCGACAAAAGATTGACGAATTAAAGATATCTGCATTGAACCAAAAT AAACTATTGGCGGATGTAAATAGGAACTTGTGGGAAAAAGTGAAAGACTACTCTGATGAAGAGCTGAAGA ACTATGGGATTTACCTTAACAAAATCAAATCACGTTATACCAAGATGACAAAAGTCTTCAATCACCAAGG AAAAGTGGCTCTGTATAACAAATTGGTGCATTCAGCTGAGAATGAGAAGAACAAACTTCAGATAAAAATA AATGAGATGGACACTATCCTCAAGAAGATCAATAACTGTCTCGCTGAAGTGGAAACAGAAACTAAGAATT TGGAGAATGAAGAGAAAAATGATGCCATGGAAGAATGGGATTCTGAAATGAGAGATGCAGAGAAAGAACT GGAACAGCTGAAAACTGAAGAAGAGGAGCTTCAGAGAAAGTTCTTAGAAGTAGAGACACAGAAAACGCAG ACCCTTGCTCAGATAGAATTTATAAAAGAACAAACAACTAAAACTGAAGAATTACTGGATCAATTGAGTT TGTCTGAGTGGGATGTCATTGAGTGGAGTGATGATCAAGCTGTATTTACCTTTGTCTATGATTCAATAGA ACTCATCATCACCTTTGGAGAGCCACTAGTTGGTCTCCCTTTTTTGGACAAGGCTTGCAGGAAGATTAAT GCACTCAGTTTTCAGTCTCTGTTAGATGAAGATAAAGCTCCTCCCTCCTCCCTTTTAGTTCACAAGCTTA TTTTTCAGTACATTGAGGAACAGGAATCCTGGAAGAAGAAATGCACAGCACAGCATCAGGTGCCCCAGAT GCTTCAAGAACTCTCACTGGTAGTGAACCATTGTAGGCTTCTTGGAGAGGAGATCGAATTTTTAAAGCGA TGGGGGCCAAATTATAGCCTAATGCATATAAATGTGAATAATACTGAATTGAGGCTTTTATTCTCCAGTT GTGCAGCATTTGCAAAGTTTGAGATAACTTTGTCTCCCTCGGCCCATTATCCCTTGGTCCCGTTACCGTT CACCATTCACAATCACATTGGGAAGACTGGCCATGATGAAATTGCTGCAATTATCTCTAAAGTGCCCTTA GAGGAAAACTACCTGAAGAATGTAGTCAAGCAAATTTACCAAGATCTACTTAAGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_029617
Insert Size	6360 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029617.2, NP_083893.2
RefSeq Size	6525 bp
RefSeq ORF	6360 bp
Locus ID	76464

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.