Kntc1 (NM_001042421) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D330012D13; jgl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225227 representing NM_001042421 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAACAATATTGAGCTGCTAACAAGCGATGACACTGGAAGTGGATGCCTGAGTGTTGGCTCAAGAA AAGAAAACGTGACTGCACTGTACCAAGCCGACTTGCTTGGGAAGATCTCCTCTGAGAAGACCTCACTGAG TCCGAAGATCCAAGCATTCAGCTTAAGCCATGGGTTTATAATCGTTGCAGACCAGTCAGTGATATTGCTT GACAGTATATGCAGATCACTCCAGTTGTTTCTTATCTTCGATACTGACGTGGATGTGGTCGGCCTGTGTC AAGAAGGAAAGTTTCTCTTTGTGGGGGAGAGAAGCGGCAACTTCCACCTTATCTACGTCACATCGAAGCA AACGCTGTTCACCAAGGCTTTTGTGGAGAAAGCTTTGGACGAGAGTCAGCGAACGTACCGGAATCTCATT ATTGAGAAAGATGGTTCAAATGAAGGTACCTATTATATGTTGCTTCTAACGAACAATGGATTTTTTTATA TTACAAACCTCCAGCTTTCACAAATTGAACAGGCCATTGAGAACACGGACTTGGATTCAGCCAAGAAGTT ACAAGGACAATTCAAATGTAGTTTTATTTCTACTGAAAATTACCACAGTTGTCTCAGTCTTGTGGCATCT CAATCTGGAACATTTGCAAGTAAAACTTCTGTGATAATTGGGGGAACCGGTAGTTGTGCATTCTCAAAAT GGGAACCCGATTCTACCAAGAAAGAAATGTCTCTTAAGAACTTTGTGGGCACAGACATTATTAAAGGTGC AAAGTCATTTCAGCTGATCGACAACCTGCTTTTTGTGCTGGATACTGATAATGTGCTGAGCTTGTGGGAT GCTTACACTCTCACTCCTGTGTGGAACTGGCCCTCTCTTCCCGTAGAGCAGTTTGTTCTCACGACAGAAG CGGATTCTCCCTCATCTGTTACCTGGCAAGGAATTACAAATCTCAAATTAGTCACCCTGACAGCAACAGC GAAGGAGAAGATGAGAAGCCTCATCATTTACTCTCTGCCTTCGATGGAAACACTGTACTCTTTGGAAGTA TCTAGTGTTTCTTCTTTAGTTCAAACAGGAATCAGCACGGACACTATATATCTTCTGGAAGGAATTCACA AAAATGATCCTAACCTATGTGAAGACTCTGTGTCTGACTTAGTACTTAGGTACCTCACAGAGGTGTTACC AGAAAACAGACTGAGCCGGTTACTTCACAAGCACAGATTTGCCGAAGCCGAGAGCTTTGCCATTCAGTTC GGACTGGATGTTGAGCTTGTTTATAAGGTCAAATCAAATGATATGTTGGAGAAGCTGGCTTTGATTTCCT CGGACAAGAGTGAGCAGTCCAAATGGCAGCAACTTGTAGATGAAGCTAAAGAAAACCTATGCAAAATCCA GGACGATGACTTCGTGGTGAATTTCTGCCTCAAGGCCCAATGGGTAACCTATGAAACCACCCAGGAAATG CTCAGTTATGCCAAAACCAGGCTCATGAAGAAAGAAGACAGAGCTCTACCTGCCTCTTCTGATGCCTTCA TGGAGGTGCTAAAAGCTCATGCAAAGTTAACTACATTTTATGGAGCATTTGGACCAGAAAAATTCAGTGG TAGCTCTTGGATTGAGTTCCTGAATAACGAAGATGACCTCAGGGATGTTTTTTTGCAGCTAAGTGAAGGA AACTTCGCTTGTGCTCAGTATCTTTGGCTTAGACATCGGGCTGACTTTGAAAGCAAGTTTGACGTGAAAA TGCTGGAGAATTTGCTGAATTCAATATCTACACAATTCCCCTTGGAAAACCTTTGTTCCTGGTTTAAAAA TGAAGTGATTCCGTTTGTGAGGAGGATTGTGCCCGAAGGACAAAACATTCTTGCAAAATGGCTAGAACAA GCATCAAGGAACCTAGAGTTAACTGATAAGGCAAACTGGCCAGAAAATGGACTTCAGCTGGCAGAGGTGT TCTTCACTGCAGAAAAAACAGACCGGTTTGGATTCGCATCTTCTTGGCACTGGATTTCCTTGGATTATCA AAACACAGAGGAAGTCCGCCAGTTACGGACACTGGTGAGCAAGTTGCGGGAGCTGATCATTCTGCATAGG AAATACAACTGCAAGCTGGCCCTCTCGGACTTTGAGAAGGAAAATGCCACCACTGTGGTGTTCCGCATGT TCGACAGGGTCTCGGCCCCAGAGCTGATCCCTTCGGTCTTAGAGAAGTCTGTGAGAGTTTACATTCGCGA ACAGAATCTGCAAGAGGAGGAGCTGCTGTTGCTCTATATAGAGGATTTACTGAAGAGGTGTAGCTCAAAG TCAATGACTCTCTTCGACACAGCGTGGGAAGCAAAGGCCATGGCCGTGATAAGATGCTTGTCTGACACAG ATCTCATCTTTGATGCTGTGCTGAAGATAATGTACAAGGCGGTGGTTCCCTGGAGTGCAGCCGTGGAGCA GCTGGTGAAACAGCACCTGGAAATGGACCACCCCAAAGTCAAGTTATTGCAGGAAAGCTACAAACTGATG GAGATGAAGAAACTTTTGCGAGGGTATGGGATAAGAGAGGTGAATCTCCTGAACAAGGAAATAATGAGAG TGATCCGATACATTCTAAAGCAGGACATCCCGTCTTCTTTAGAAGATGCTCTGAAGGTGGCCCAGGGGTA CAGGCTGTCTGATGACGAAATCTACAGCCTGAGAATCATCGATCTGATTGACAGGGAGCAGGGGGGAGAT TGCCTCCTGCTACTGAAGTCTCTGCCTGCCGCTGAAGCCGAGAAGACTGCAGAAAGGGTTATCATCTGGG CTCGACTGGCACTGCAGGAGGAGCCTGACGGCTCTGAGGAGGACAAGGCCTGGAGAATATCTGTGGCGAA GACATCGGTGGATATTCTCAAGATCCTGTGTGATATTCGAAAAGACAATCTGCAGAAGAAGGATGAATCT GAAGAATTCCTGAAGCGATTTCAAATGGTTGCTAGTTTACAGGAGAACTTTGAGGTCTTCCTTCCATTTG AAGACTACAGCAACACCGCCTTGGTAGCTGGCCTTCGAGAACAGTATATTAAGGCACAGGAAGCTGCACA GGCTGAGCACAAACACAGGGGACCCCCCGGGCCCACCCCGGCTAGGGGGACACACCTGAGCATCAAGTCA AAGCTGCACCGACAGGCACTGGCCCTGCAGGTGTCTGAACAGGAGCTGGAGGCAGAGCTGACCTTGAGAG CCTTAAAGGACGGGAAGGTGGTGGCCGCCCTGAGCAAATGCAGAGATTTACTTAAGCATTACTGCAATGC CGACACTGGGAGGTTACTGTTTGTGGTGTGTCAGAAGCTCTGTCAGATGTTGGCTGACGATGTCCCAATG GTGGCCCCAGGAGGACTCAGCCTTCCTTCTGAGATCCATGACCTCGCATGCCACGCTGTCACCATCTGCA GTCCAGATTATTTGCTGGATGTTTTAGAACTAAGTAAATACACTTTAACCGCTGTGGAGCTTTGCAGACA ATGCCAGATGGATGACTGTGGAATGCTGATGAAGGCCGCTCTTGGAACTCACAAGGATCCCTATGAAGAG TGGTCCTTTAGTGACTTCTTCAGTGAAGATGGAATTGTTCTCGAGTCCCAGGTGGTGCTGCCTGTCATCT ATGAATTGATCTCATCAGTGATGCCCCCTGCAGAAAGCAAAAGACATCCTTTGGATTCTATAAGTTTGCC ATACTGCTCCACTAGTGAAGGAGAAAACCGTATTCTCCCTCTTGTAAGCTCCATCTCTGCTCTGCTCCGG AGCCTCCAGGAATGTAGTCAGTGGGAGCTAGCCCTGAGGTTCGTGGTGGGCTCCTTTGGTACCTGCCTGC AGCACTCTATGTCAAACGTCATGAGTATCAGCTTGAGTAAACAGTTACTTGGGAAGAACACATTGGCGAA CTCCAGGCATATCATTATGGAATTGAAAGAGAAGTCCATCACATTTATCAGAGAAAATGCTACGACACTC CTGCATAAAGTGTTTAACTGTCGGGTGGTGGATCTGGACCTCGCCTTGGCTTACTGCACTCTCTTACCTC AAAAGGATGTATTCGACAACCTCTGGAAGTTCATAGATAAAGCGTGGCAGAATTACGACAAAATTCTGGC ATTATCTTTGGTGGGCTCTCAGCTGGCAAATCTCTACCAGGATATTGAAACAGGACTTTGGTTCCACGAG CTCAGTATCGATGCCAAGTGGGGTATTCGTCTTGGTAAACTTGGTATTTCTTTTCAGCCTGCTTTTAGAC AAAATTTTCTCACCAAGAAAGACCTTATCAAAGCTCTTGTGAATAACATAGACATGGACACCAGCCTCAT TCTGGAGTATTGCAGCACATTTCAGCTGGACTCTGACGCTGCCCTTCGGCTGTTCATTGAAACTCTGCTC CGCAACACTAGCAGCCAGAGCCAGGGAGATGCTGCCCCGGAGTCCACAAAGCATCAGCATTCCAAACTCC TGGCGAAAGCCACTGAGCTGGTCCCTTTACTGAAGAACACAAAGGATCTGGTGATCAGTCTTAGTGAGAT CCTCTATAAGCTGGACCCATATGATTATGAAATGATTGATGTTGTCCTGAAAGTTTTAGAACAAGCTAAC GAAAAGATAACCAGTGTGAACATCAATCAGGCGCTGAATCTTCTGAGACACTTGAAGTCATACAGAAGGA TCTCCCCGCCAGTGGACCACGAATACCAGTACGCGCTGGAGCACATGATCACTCTGCCACCGGCTGCTCA CACTAGACTGCCCTTTCACCTAATACTGTTTGGCACAGCACAGAACTTCTGGAAAATTCTCTCCTCAGAG CTCAGCGAAGAGTCGCTGCCAACCCTGCTCTTAATCGCGAAGCTGATGAAGTTCTCCCTAGATACTCTCT ATGTGTCTACCGCCAAACACTTGTTTGAAAAAAACCTGAAGCCAAAGCTCCTGAAGTCGGCACAAGCCAG GTCCTCCACCCTGATGAGCAAGGAAGTGGATAAGCTCATGCAGACGCTGGAGTCGTACCTCCTGTCCATC GTCAACCCCGAGTGGGCTGTCGCCATTGCCATCAGCCTCACCCAGGAAGTCCCAGAAGGCCCCTTCAAGA TGTCTAGTTTGAAGTTCTGCCTGTATTTAGCTGAGAGATGGCTACAAAACATCCCACCTCAGGATGAGAC GTGTGAAAAGGCCAAAGCCTTGCAGAAGAAGCTCTGCCTCCAGGTCCGGCTCTCAGGAACGGAAGCCGTG CTCATAGCCCACAAGCTGAACGATCAGGAATATTTAAGAGTGATAGGGAAGCCAGCGCACCTCATCGTGA GCCTCTACGAACATCCCAGCATAAGCGAAAGGCTTTGTACCACATCTGGCAAAGATTATCCCGATATCCA TACAGCCGCTAAAGAAATAGCCGAAGTCAATGAAGTTAATTTGGAAAAAATCTGGGACATGTTGTTGGAG AAATGGCTGTGCCCCTCAACGGTGCCCAGTGAGAAAGCATCGGAGTTCTTTGAGCTGGAAGAGGATGAAG TGCTGCATAGAGTCGTGTATCTCCTCCAGGCTCGTCCTGTTGATTATTGTTCGAGAATGCTGTTTGTATT TGCCACTTCAGCTACAAGTACACTGGGCATGCGCCAGCTAACGTTTGCCCACAAAGCACGAGCTCTGCAG TGTCTCCTCTATTTGGCTGACAAGGAAACAATTGAATCTCTCTTCAAAAAGCCCATTAAAGAAATGAAAT CCTATCTGAAATGTATAACTTTCCTGGCATCATTTGAGACATTGAATATTCCCATCACATATGAATTATT CTGCAACAGTCCCAAAGAAGGCATGATTAAGGGCTTGTGGAAGAACCACAGCCACGAACCTATGGCAGTA CGATTGGTTGCAGAGCTTTGCTTAGAATACAAAATCTATGACCTGCAGCTTTGGAATGGGCTCTTGCAGA AGCTTCTGGGCTTCAACATGATTCCTTACCTGAGGAAAGTGTTATCCTGCATTTCTAGCATCCATTCGTT ATGGCAGGTGCCCTACTTCAGCAAAGCGTGGCAGCGCGTGATCCAGATCCCTCTGCTCTCGGCTTCCTGT CCTTTAAGACCCAGTCAGTTAGCAGACTGCTGTGACAGTCTCGTCGCCATCCTTGAATGTCCAGTTTCAG ATGACCTGGACATGATGGGAGTCGCCAAGCAGTACGTCCAGTTAGACCTTCCCGCTTTTGCTCTGACCTG CCTGACGCTCATGCCCCACTCTGAAAAAAGACACCAGCAGATCAAGAATTTTCTGAATTCCTGTGATGCT CGGATCATTTTACAGCAGATAGAAGAACATATGAACACTGGCCAGCTAGCAGGGTTTTCACATCAGATTG GAAGTCTGGTTCTGAATCATGTTGTAAATAAGAAGGAATTTGGGATTTTGGCAAAGACAAAGTATTTTCA GTTGTTGAAATGCCACGTGATAAACACCGGCAACGTCACTGAATTGGTAAACTATTTGGCAAATGACTTC AGTGTAGATGAAGCATCAGCGTTGATAAATGAGTATTCAAAGCATTGTGGGAAGCCTGTGCCAGCAGATG CAGCTCCCTGTGAGATCCTGCAGACATTTCTTGGTGGATCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001042421
Insert Size	6624 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001042421.1, NP_001035886.1
RefSeq Size	6961 bp
RefSeq ORF	6624 bp
Locus ID	208628
UniProt ID	Q8C3Y4
Gene Summary	Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (By similarity).[UniProtKB/Swiss-Prot Function]

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