Pard6a (NM_001286344) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0710008C04Rik; 2610010A15Rik; Par-6; PAR-6A; Par6; PAR6alpha; Par6c; TAX40; Tip-40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226049 representing NM_001286344 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTTCGCGTGGCTCCCCAGGGCCTGGAGCGGGTTCCAGGTATCTTCATCTCCCGCCTGGTACGTG GGGGCCTGGCTGAGAGTACAGGGCTGCTGGCGGTCAGTGATGAGATCCTTGAGGTCAACGGCATTGAGGT GGCCGGGAAGACCTTGGACCAAGTGACGGACATGATGGTCGCCAACAGCCACAACCTCATCGTCACTGTC AAGCCTGCCAACCAGCGTAATAATGTGGTACGGGGGGCATCTGGGCGTCTGACAGGGCCTTCCTCTGTAG GGCCTGGGCCTACTGATCCTGACAGTGACGATGACAGCAGTGACCTGGTCATTGAGAATCGCCACCCTCC CTGTTCTAATGGGCTGTCTCAGGGGCCCCTGTGCTGGGACCTGCAACCTGGCTGCCTACTTCCTGGTGCT GGCAGCTCTCTGCCCTCCTTGGATAGCAGAGAGCAAGCCAATTCTGGCTGGGGGAATGGCATGCGAGGTG ATGTTAGCGGATTCAGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286344
Insert Size	513 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286344.1, NP_001273273.1
RefSeq Size	1266 bp
RefSeq ORF	513 bp
Locus ID	56513
Gene Summary	Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:15761148). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon. This encodes a shorter protein (isoform 4), compared to isoform 1.

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