Kdf1 (NM_001083916) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1810019J16Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227481 representing NM_001083916 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGCCGGGACAGCCCCGCCCATCATCTGGGCCTCCACGCTTGGGGCCCTGGGAGAGGCCATCAG AGTTATGCCTGGAAACGAACGATGAGCGCTCCCAGCCCCCACCGGGCCGTCGCACCAGGAGGCCAGACCC TAAGGACCCTGGCCACCACGGGCCAGAGAGTATCACCTTCATTTCAGGCTCTGCAGAACCAGCCAACGAG CCCCCAACCTGCTGCCTCCTCTGGCGCCCCTGGGGTTGGGACTGGTGTAGGGCTGCCTTCTGCTTCCGAC GCTGCAGGGATTGCCTGCAGCGCTGTGGGGCTTGTGTGCGGGGCTGCAGCCCCTGCTTGTCTGCCGGAGA CCCCATTGAAGGGTCTGCCGAAGCCGCCTGGGCCAAGGAACACAATGGTGTGCCCCCCAGCCCAGACCGT GCACCCCCCAGCCGCCGGGATGGCCAGAGGCTCAAGACAAGCATGGGCAGCAGCTTCAGCTACCCTGATG TTAAGCTCAAAGGCATCCCTGTCTACCCCTACCGCCATGCCACCTCCCCAGTCCCTGACGTGGACTCCTG CTGCAAGGAGCCCCTGGCCGAGCCTCCTCCCACGCGGCACAGCTTGCCTAGCACCTTCACCAACAGCCCC CGCGGCTCTGAGGAGTACTACTCCTTCCATGAATCGGACCTGGACCTGCCTGAGATGGGCAGTGGCTCCA TGTCGAGCCGGGAGATCGACGTGCTTATTTTCAAGAAGCTGACAGAGCTGTTCAGCGTGCACCAGATTGA CGAGCTGGCCAAGTGCACATCGGACACCGTGTTCCTGGAGAAGACCAGCAAGATCTCAGACCTGATCAGC AGCATCACGCAGGACTACCACCTGGATGAGCAAGACGCCGAGGGCCGCCTGGTGCGGGGCATCATCCGTA TTAGTACCCGCAAAAGCCGCTCCCGCCCACAGACCTCCGAGGGGCGCTCAGCCCGCTCTACTGCCCCTGC TGCTGCCCCCGACAGTGGCCATGAGACCATGCTGGGCTCTGGCCTCAGCCAGGACGAGCTGACAGTCCAG ATCTCCCAGGAGACAACCGCAGATGCCATCGCCAGGAAACTGAGGCCGTATGGAGCCCCAGGGTACCCAG CCAGCCAAGACTCATCATTCCAGGGCACGGACACAGACTCTTCAGGGGCACCCCTGCTGCAGGTGTACTG CTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001083916
Insert Size	1194 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001083916.1, NP_001077385.1
RefSeq Size	1804 bp
RefSeq ORF	1194 bp
Locus ID	69073
UniProt ID	A2A9F4
Gene Summary	Plays a role in the regulation of the epidermis formation during early development. Required both as an inhibitor of basal cell proliferation and a promoter of differentiation of basal progenitor cell progeny.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

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