Glra1 (NM_001290821) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	nmf11; oscillator; ot; spasmodic; spd
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227795 representing NM_001290821 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAGCTTCAATACTCTGCGATTCTACCTTTGGGAGACCATTGTATTCTTCAGCCTTGCTGCTTCCA AAGAGGCTGAAGCCGCCCGCTCCGCACCCAAGCCTATGTCACCCTCGGACTTCCTGGATAAGCTCATGGG GAGGACTTCTGGGTATGACGCCAGGATCAGGCCCAACTTTAAAGGTCCTCCTGTGAATGTAAGTTGCAAC ATCTTCATCAACAGTTTCGGTTCCATCGCTGAGACAACCATGGACTATAGGGTCAACATCTTCCTGAGGC AGCAGTGGAACGACCCCCGTCTGGCCTACAATGAATACCCTGATGACTCTCTGGACCTTGACCCATCTAT GTTGGATTCCATCTGGAAGCCTGACTTGTTCTTTGCCAATGAGAAGGGGGCCCACTTCCACGAAATCACC ACGGACAACAAACTGCTAAGAATCTCCCGGAATGGCAATGTCCTCTACAGCATCAGAATCACCCTGACGC TGGCCTGCCCCATGGACCTGAAGAATTTCCCGATGGATGTACAGACGTGTATCATGCAACTCGAAAGCTT TGGATATACCATGAACGACCTCATCTTTGAGTGGCAGGAGCAAGGAGCTGTGCAGGTGGCAGACGGACTG ACCCTGCCTCAGTTTATTCTGAAGGAAGAGAAAGACCTGAGATACTGCACCAAGCACTACAACACAGGTA AATTCACCTGCATCGAGGCCCGATTCCACCTGGAGCGGCAGATGGGCTACTACCTGATCCAGATGTACAT CCCCAGCCTGCTCATCGTCATCCTGTCCTGGATCTCCTTCTGGATCAACATGGATGCTGCACCAGCTCGT GTGGGGCTGGGCATCACCACAGTGCTCACCATGACCACACAGAGCTCTGGCTCCCGAGCCTCCCTACCCA AGGTGTCCTACGTGAAAGCTATTGACATTTGGATGGCTGTTTGCCTGCTCTTCGTGTTCTCTGCCCTGCT GGAGTACGCCGCCGTCAACTTTGTGTCTCGGCAACACAAGGAACTGCTTCGATTTAGGAGGAAGAGGCGA CATCACAAGAGCCCCATGCTAAATCTGTTTCAGGATGATGAGGGTGGAGAAGGCCGCTTCAACTTCTCTG CCTATGGGATGGGCCCAGCCTGTCTGCAGGCCAAGGATGGCATCTCTGTCAAGGGTGCCAACAACAACAA CACCACTAACCCGCCTCCTGCGCCATCCAAGTCCCCGGAGGAGATGCGGAAACTCTTCATCCAGAGAGCC AAGAAGATCGACAAGATATCTCGCATCGGTTTCCCCATGGCCTTCCTCATCTTCAACATGTTCTACTGGA TCATCTATAAGATCGTCCGGAGAGAGGATGTCCACAACAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290821
Insert Size	1374 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290821.1, NP_001277750.1
RefSeq Size	2413 bp
RefSeq ORF	1374 bp
Locus ID	14654
UniProt ID	Q64018
Gene Summary	Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:16672662, PubMed:17114051, PubMed:24801766). Channel opening is also triggered by taurine and beta-alanine (By similarity). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization (By similarity). Plays an important role in the down-regulation of neuronal excitability (PubMed:9145798). Contributes to the generation of inhibitory postsynaptic currents (PubMed:16672662, PubMed:17114051, PubMed:24801766). Channel activity is potentiated by ethanol. Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol (PubMed:24801766).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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