Far1 (NM_001285831) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2600011M19Rik; 2900034E22Rik; 3732409C05Rik; AI850429; Mlstd2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228126 representing NM_001285831 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTCAATCCCAGAATACTACGAGGGGAAGAATATCCTTCTCACAGGAGCCACTGGTTTCTTAGGCA AGGTGCTTCTGGAAAAGTTGCTGAGATCTTGTCCCAGGGTGAATTCTGTCTATGTTTTGGTGAGGCAAAA AGCTGGTCAGACACCCCAAGAGCGAGTGGAGGAAATCCTCAGTAGCAAGCTTTTCGACAGGTTGAGAGAT GAAAATCCAGATTTTAGAGAGAAAATTATAGCGATCAACAGTGAACTTACCCAACCTAAACTGGCTCTCA GTGAAGAAGATAAGGAGATCATTATAGATTCTACAAATGTCATATTCCACTGTGCGGCTACTGTAAGATT TAATGAAAATTTAAGAGATGCTGTCCAGTTAAATGTGATAGCTACACGACAGCTTATTCTCCTTGCACAG CAAATGAAGAATCTGGAAGTGTTCATGCATGTCTCAACAGCGTATGCCTACTGCAATCGAAAGCATATTG ATGAAGTAGTTTATCCACCCCCTGTAGATCCCAAGAAGCTGATTGATTCTTTAGAATGGATGGATGATGG CCTAGTAAATGATATCACACCAAAATTGATAGGCGACAGGCCTAACACGTACATATACACCAAGGCATTG GCAGAGTATGTTGTGCAGCAGGAAGGAGCAAAGCTAAATGTTGCCATTGTAAGGCCGTCGATCGTCGGCG CCAGTTGGAAAGAACCTTTTCCAGGATGGATTGATAACTTTAATGGACCAAGTGGTCTCTTCATTGCGGC AGGGAAAGGAATTCTTCGAACAATGCGTGCCTCCAACAATGCCCTTGCAGATCTCGTTCCTGTAGATGTA GTGGTCAACACGAGCCTTGCTGCAGCCTGGTATTCTGGAGTTAATAGACCAAGAAACATCATGGTGTATA ATTGCACAACAGGCAGCACTAATCCTTTCCACTGGGGGGAAGTTGGAGATTATCTAAATCATTCCTTCAA GATGAATCCTTTAAACCAAGTATTCAGGCACCCCTATGTTAAATTCTGTTCCAACAACCTAATGCTTCAT TATTGGAAGGGTGTCAAACATACAGTACCTGCATTATTGCTCGATCTTGCTCTCAGGTTGACAGGTCAGA AACCGTGGATGATGAAAACAATCACTCGTCTTCACAAAGCCATGGTATTCCTTGAGTATTTTACAAGTAA CTCTTGGGTTTGGAATACTGATAATGTCAATATGTTAATGAACCAACTAAACCCTGAAGATAAAAAGACC TTTAATATTGATGTACGGCAGTTACATTGGGCAGAATATATAGAAAACTACTGCATGGGGACTAAGAAGT ACGTGTTGAATGAAGAGATGTCTGGCCTCCCTGCTGCTCGGAAGCATCTCAACAAGTTGCGGAATATCCG TTATGGTTTCAATACAATCCTTGTGATACTTATCTGGCGCATTTTTATTGCAAGATCACAAATGGCAAGA AACATCTGGTATTTTGTGGTTAGTCTGTGTTACAAATTTCTGTCATACTTCCGAGCATCCAGCACTATGA GATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285831
Insert Size	1548 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285831.1, NP_001272760.1
RefSeq Size	4339 bp
RefSeq ORF	1548 bp
Locus ID	67420
UniProt ID	Q922J9
Gene Summary	Catalyzes the reduction of saturated and unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols (PubMed:15220348, PubMed:15220349). It plays an essential role in the production of ether lipids/plasmalogens which synthesis requires fatty alcohols (By similarity). In parallel, it is also required for wax monoesters production since fatty alcohols also constitute a substrate for their synthesis (PubMed:15220349).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate exon in the coding region compared to variant 1. The resulting isoform (2) is the same length and has the same N- and C-termini compared to isoform 1 but differs at an internal segment. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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