Dync1i2 (NM_001198873) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110079H08Rik; AW554389; Dncic2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228578 representing NM_001198873 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACAAAAGTGATTTAAAAGCTGAGTTGGAACGTAAGAAGCAACGGTTGGCCCAAATCAGAGAAG AAAAGAAGAGGAAAGAAGAGGAAAGGAAAAAAAAGGAAACTGATCAGAAGAAGGAAGCTGCTGTTTCTGT ACAAGAAGAGTCTGATCTTGAAAAAAAAAGAAGAGAAGCTGAGGCATTGCTTCAAAGCATGGGACTAACT ACGGACTCCCCTATTGAGGATTCTAGCTTCAATCAAGTTGACATAAAACTAGTTTTTTCTGAGTACTGGG TCCCTCCTCCTATGTCTCCATCCTCCAAGTCGGTGAGCACGCCAAGTGAAGCTGGAAGCCAGGATTCTGG AGATGGCGCCGTGGGATCTAGGACGCTGCATTGGGATACAGATCCATCAGTTCTTCAGCTTCACTCAGAT TCCGATTTGGGACGAGGACCTATTAAACTTGGAATGGCCAAAATTACTCAAGTTGACTTTCCCCCTCGAG AAATCGTCACATACACAAAGGAAACTCAGACCCCGGTTACAGCTCAACCCAAAGAAGATGAGGAGGAGGA AGATGATGTAGCAACTCCTAAACCTCCTGTTGAACCTGAAGAAGAGAAAACTTTGAAAAAAGACGAAGAG AATGATAGCAAAGCTCCACCTCATGAACTAACTGAAGAAGAAAAGCAACAGATTTTACACTCAGAAGAAT TTTTAAGTTTCTTTGACCATTCTACGAGAATTGTAGAAAGAGCCCTTTCTGAGCAGATTAACATCTTCTT TGACTACAGTGGGAGAGACTTGGAAGACAAAGAAGGGGAGATTCAAGCAGGTGCTAAGCTGTCATTAAAT CGACAGTTCTTTGATGAGCGTTGGTCAAAGCATCGAGTTGTTAGTTGTTTGGATTGGTCATCCCAGTATC CAGAATTACTTGTGGCTTCCTATAATAACAATGAAGAGGCTCCTCATGAGCCTGATGGTGTGGCCCTCGT GTGGAATATGAAGTACAAAAAAACTACCCCAGAGTATGTGTTCCACTGCCAGTCAGCTGTAATGTCTGCT ACATTTGCAAAATTTCATCCGAATCTTGTTGTTGGAGGTACATATTCAGGCCAGATTGTGCTTTGGGATA ACCGCAGCAATAAAAGAACTCCTGTCCAGAGAACGCCGCTGTCAGCTGCTGCACACACACACCCTGTCTA CTGTGTAAATGTTGTTGGAACACAAAATGCTCACAATCTGATTAGCATCTCTACTGATGGAAAAATTTGT TCCTGGAGTTTGGACATGCTTTCCCATCCACAGGATAGCATGGAGTTGGTTCATAAACAGTCAAAGGCAG TAGCTGTAACATCTATGTCTTTCCCTGTTGGAGACGTCAACAACTTTGTTGTGGGCAGTGAAGAAGGTTC TGTGTATACAGCATGCCGCCATGGCAGCAAAGCTGGAATCAGTGAGATGTTTGAGGGACACCAGGGACCC ATCACTGGAATCCACTGTCACGCGGCTGTTGGAGCAGTAGACTTCTCACATCTGTTTGTCACTTCGTCTT TTGACTGGACAGTTAAACTTTGGACAACTAAGAATAACAAGCCTTTGTACTCATTTGAAGATAATTCAGA CTATGTCTATGATGTTATGTGGTCACCCACCCACCCAGCCCTGTTTGCCTGTGTGGACGGCATGGGGAGA CTGGATCTATGGAATCTCAACAATGACACAGAGGTACCAACTGCCAGCATTTCTGTGGAGGGCAACCCTG CTCTTAACCGTGTGAGATGGACCCACTCAGGAAGAGAAATTGCCGTGGGTGATTCTGAAGGACAGATTGT CATCTATGATGTGGGAGAGCAGATCGCTGTTCCCCGAAATGATGAATGGGCAAGATTTGGCCGAACACTC GCAGAAATTAACGCAAACCGAGCTGACGCAGAGGAGGAGGCAGCTACCCGAATCCCTGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198873
Insert Size	1953 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198873.1, NP_001185802.1
RefSeq Size	2659 bp
RefSeq ORF	1953 bp
Locus ID	13427
Gene Summary	Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2; also called 1a.E) uses an alternate splice acceptor site in the 5' coding region, compared to variant 1. Variant 2 encodes a shorter isoform (2), compared to isoform 1. Variants 2 and 7 encode the same protein.

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