Trim2 (NM_001271725) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mKIAA0517; narf
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228905 representing NM_001271725 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGAGTGGCCGTTATGGAACCCAGCAGCGTGCAGGGTCGAGGACAGCCGGCCCCCCATGTCAGT GGTCTAGGATGGCCAGTGAGGGCGCCAGCATCCCAAGTCCCGTGGTGCGCCAGATTGACAAGCAGTTTCT GATCTGCAGTATATGCCTGGAACGGTACAAGAACCCCAAGGTCCTGCCCTGTCTGCACACTTTCTGCGAG AGGTGCCTGCAGAACTACATCCCAGCCCACAGTTTAACCCTCTCCTGCCCGGTGTGTCGCCAGACATCCA TCCTGCCCGAGAAAGGGGTGGCCGCACTTCAGAACAACTTCTTTATCACGAACCTCATGGATGTGCTGCA GCGGACGCCAGGCAGCAACGGCGAGGACTCTTCCATCCTGGAGACGGTCACCGCTGTGGCTGCAGGAAAG CCTCTTTCGTGCCCAAACCATGATGGAAATGTGATGGAATTTTACTGCCAGTCCTGTGAGACCGCCATGT GTCGGGAGTGCACGGAGGGGGAGCACGCGGAGCACCCCACAGTCCCGCTGAAGGATGTGGTGGAGCAGCA CAAGGCCTCGCTCCAGGTCCAGCTGGACGCAGTCAACAAAAGACTCCCAGAAATAGATTCTGCTCTTCAG TTCATCTCAGAAATCATCCATCAGTTAACCAACCAAAAGGCCAGCATTGTGGATGACATCCACTCCACCT TTGATGAGCTGCAGAAGACTTTAAATGTTCGCAAGAGCGTGCTGCTCATGGAGTTGGAGGTCAACTATGG TCTCAAACACAAAGTGCTGCAGTCGCAGCTGGATACCCTGCTCCAAGGCCAGGAAAGCATTAAGAGCTGC AGCAACTTCACGGCTCAGGCCCTCAACCATGGCACGGAGACGGAGGTGCTGCTGGTGAAGAAGCAGATGA GCGAGAAGCTGAACGAACTGGCGGACCAGGACTTCCCACTGCACCCGCGCGAGAACGACCAGCTGGATTT TATCGTGGAAACGGAGGGACTGAAGAAGTCTATCCACAACCTGGGGACCATCCTCACCACTAACGCGGTC GCCTCTGAGACGGTGGCCACGGGGGAGGGGCTGCGGCAGACCATCATCGGGCAGCCCATGTCCGTTACTA TAACCACCAAGGACAAAGACGGAGAGCTGTGCAAGACAGGCAACGCATACCTCACCGCTGAGCTCAGCAC CCCGGACGGCAGCGTGGCCGACGGAGAGATCCTGGACAACAAGAACGGCACCTACGAGTTCTTGTATACT GTGCAGAAGGAGGGGGACTTCACCCTGTCCCTCAGGCTCTACGACCAGCATATCCGAGGCAGCCCGTTTA AGCTCAAGGTCATCCGCTCTGCCGACGTGTCCCCCACCACCGAGGGCGTGAAGCGACGAGTCAAGTCCCC GGGGAGCGGGCACGTCAAGCAGAAGGCTGTGAAGAGACCTGCCAGCATGTACAGCACCGGCAAGCGAAAA GAGAACCCCATCGAGGACGACCTGATCTTCCGAGTCGGTACCAAAGGAAGAAACAAAGGAGAATTTACAA ACCTGCAGGGTGTGGCTGCATCTACAAGTGGAAAGATATTAATTGCAGATAGTAACAACCAGTGTGTGCA GATCTTTTCCAATGATGGACAGTTCAAAAGTCGTTTCGGCATCCGAGGACGATCACCCGGACAGCTCCAG CGGCCCACGGGGGTAGCGGTGCACCCCAGTGGGGACATCATCATTGCAGATTATGACAACAAGTGGGTTA GCATTTTCTCCAATGATGGGAAGTTTAAGACAAAAATTGGATCAGGAAAACTGATGGGGCCCAAAGGAGT TTCTGTGGACCGCAATGGGCACATTATTGTGGTGGACAACAAGGCGTGCTGTGTGTTTATCTTCCAGCCC AACGGGAAAATAGTCACCAGGTTTGGTAGCCGAGGAAACGGGGACAGGCAGTTTGCAGGTCCCCACTTTG CAGCTGTGAATAGCAATAATGAGATCATCATTACAGATTTCCATAATCACTCTGTCAAGGTATTTAATCA GGAAGGAGAATTCATGTTGAAGTTTGGCTCCAATGGAGAAGGAAATGGGCAGTTTAATGCACCAACAGGT GTGGCCGTGGACTCAAATGGGAACATCATTGTGGCTGACTGGGGGAACAGCAGGATCCAGGTTTTTGATG GGAGTGGATCATTTTTGTCCTATATCAACACATCTGCTGACCCGCTCTATGGCCCCCAGGGCCTGGCTCT CACTTCAGATGGCCATGTTGTGGTTGCAGACTCCGGAAACCACTGTTTCAAGGTCTACCGGTACTTACAG TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271725
Insert Size	2313 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271725.1, NP_001258654.1
RefSeq Size	7108 bp
RefSeq ORF	2313 bp
Locus ID	80890
UniProt ID	Q9ESN6
Gene Summary	UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1).

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