Eml1 (NM_001286347) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110008N23Rik; A930030P13Rik; AA171013; AI847476; AI853955; ELP79; EMAP; EMAPL; heco
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228963 representing NM_001286347 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGTCAGACCGCATCGCCTCTCTGGAGCAGCGCGTCCAGATGCAGGAGGATGACATTCAACTGC TCAAGTCAGCGCTGGCCGACGTGGTTCGGAGGCTGAACATCACGGAGGAGCAACAGGCTGTGCTCAACAG GAAAGGACCTACCAAAGCGAGGCCACTGGGGCAGACCCTGCCATTGAGAACCACCGTCAACAATGGCACC GTGTTACCAAAGAAACCCAGTGCCTCCCTCCCGGCACCCTCCGGGGCCAGGAAAGAAGTAGTTGTGCCGG TAACCAAAAGCATCAATAGGACCAGCTCTTCCGAAAGAGTGTCTCCAGGTGGCCGGAGGGAGAGCTCTGG GGACTCCAAAGGAAGCCGGAACCGCACGGGCTCAACCAGTAGCTCCTCCAGCGGCAAGAAGAACAGTGAG AGCAAACCCAAGGAGCCCGCATTCAGTCCAGCCCTTCAGTCTCCGAAACCCCAAGGGAAGCGCCGGGTGA CACACTGCAAAGAAGAAGGATATGTAAAAATGTTTCTTCGAGGCCGTCCGGTCACCATGTACATGCCCAA AGACCAAGTGGATTCGTACAGTTTGGAAGCAAAAGCTGAGTTACCAACGAAGCGGCTGAAACTGGAGTGG GTCTACGGGTACCGGGGGCGAGACTGTCGCAATAACTTGTACTTGCTCCCGACCGGGGAGACCGTGTACT TCATTGCGTCTGTCGTGGTGCTTTACAATGTGGAGGAGCAGCTGCAGAGGCATTACGCGGGCCACAACGA TGACGTCAAGTGCCTAGCGGTCCATCCTGACAGGATCACCATAGCAACGGGACAAGTGGCGGGCACATCT AAGGATGGAAAGCAACTGCCACCACATGTGCGCATCTGGGACTCTGTGACACTGAACACTCTGCATGTCA TTGGAATAGGCTTTTTTGACCGGGCTGTCACCTGCATCGCATTCTCAAAGTCTAACGGAGGAGGCCATCT CTGTGCTGTGGATGATTCCAACGATCACGTGCTGTCCGTGTGGGATTGGCAGAAAGAAGAGAGACTGGCC GACGTGAAGTGTTCGAACGAAGCGGTATTTGCGGCGGACTTCCACCCCACAGACACCAATATCATAGTCA CCTGCGGGAAGTCACATCTCTACTTTTGGACCCTAGAAGGAAATTCCCTTAACAAGAAGCAAGGGTTGTT TGAGAAACAAGAGAAGCCAAAGTTTGTTCTCTGCGTGACGTTTTCTGAAAATGGCGACACCATTACTGGA GATTCAAGCGGCAACATCTTGGTGTGGGGGAAAGGTACAAATCGGATAAGCTATGCAGTTCAAGGGGCCC ACGAGGGCGGCATTTTTGCACTTTGTATGCTGAGAGACGGGACGCTGGTGTCCGGAGGAGGGAAGGACCG GCGACTCATTTCCTGGAACGGAAACTATCAAAAACTTCACAAAGCAGAGATTCCTGAGCAGTTTGGCCCA ATACGGACAGTAGCCGAAGGGAAGGGCAACGTCATCTTGATAGGCACTACTAGAAACTTTGTCCTGCAAG GCACCTTGTCAGGGGACTTCACACCCATCACTCAGGGCCACACCGATGAGCTCTGGGGGCTAGCCATCCA TGCCTCCAAGCCTCAGTTCCTGACTTGTGGACATGACAAACATGCCACTCTCTGGGACGCTGTCGGTCAC CGGCCAGTCTGGGACAAAATCATAGAGGATCCAGCTCAGTCCTCTGGTTTTCATCCTTCCGGGTCTGTGG TTGCAGTGGGGACACTGACTGGGAGGTGGTTTGTGTTTGACACGGAGACAAAGGACTTGGTCACCGTCCA CACGGATGGGAATGAGCAGCTGTCCGTGATGCGGTATTCTCCAGATGGGAACTTCTTAGCAATCGGCTCC CATGACAACTGCATCTACATATATGGAGTTACCGACAATGGAAGGAAGTACACACGAGTTGGCAAGTGCT CCGGCCATTCCAGCTTCATCACCCACTTGGACTGGTCCGTGAACTCACAATTCCTGGTGTCAAATTCCGG GGACTACGAGATCCTCTACTGGGTTCCGTCTGCCTGTAAGCAAGTCGTGAGTGTGGAAACCACAAGGGAC ATCGAGTGGGCCACCTATACCTGCACCTTGGGATTCCACGTCTTTGGAGTGTGGCCGGAGGGCTCCGATG GGACAGACATCAACGCCGTCTGCCGGGCTCACGAGAGAAAGCTCTTGTGCACAGGCGATGACTTCGGCAA AGTGCACCTCTTCTCATACCCGTGCTCACAGTTCCGGGCTCCAAGCCACATCTACAGTGGACACAGCAGC CACGTCACCAACGTGGACTTCCTCTGTGAAGACAGCCACCTTATCTCCACGGGTGGGAAAGACACAAGCA TCATGCAGTGGCGAGTCATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286347
Insert Size	2403 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286347.1, NP_001273276.1
RefSeq Size	3857 bp
RefSeq ORF	2403 bp
Locus ID	68519
UniProt ID	Q05BC3
Gene Summary	Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, initiates translation at a downstream start codon, and contains an additional internal in-frame exon, compared to variant 1. The resulting isoform (4) is shorter; it has a shorter N-terminus and an additional internal segment, compared to isoform 1.

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