Dsc3 (NM_001291809) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5430426I24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229028 representing NM_001291809 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTTCCTGAGTTCCGGAGCCCACAGTGCAGGGCACTATGCACGAAGCTGCTGCTGACCCTCTGGG TCTTCAGTTTTGTTGGTGAAGCCTGTAAAAAGGTGACATTTCATGTACCTTCAACACTAGAGGCAGACAA GATCATCGGAAGAGTTAGTTTGAAAGAGTGTCTCAGCTCGGCAGATGGCATCATGCCAAGTGATCCTGAT TTCAGAGTTCTAGATGATGGGTCAGTTTATCCGACCAGAGCTGTTGTATTGTCTGATGAGAAAAGATCTT TTACCATTCAGCTTTCTGATTCAAAGATGCAGACACAGAAAGAGATCCCTGTAATCCTGGAGCACAAGAA GAAGGTACTGAAGAAAAGACACACTAAGGAAACTGTCCTCAGAAGATCTAAGAGAAGATGGGCACCCATT CCATGTTCAATGCAAGAAAATTCCTTGGGTCCCTTCCCATTGTTTCTTCAACAGGTTCAGTCTGATGCAG CTCAGAACTACACAGTCTTCTATTCAATAAGTGGGCGTGGAGCTGATCAAGAACCACTTAACTGGTTTTT CATCGAGAGAGACACTGGGAATCTATATTGTACTCGGCCTGTGGATCGTGAGGAATATGATGTTTTTGAT TTGATTGCCTATGCATCCACTGCAGATGGCTATTCAGCAGATTTACCTCTCCCACTGCCCATCAAAATAG AGGATGAAAATGACAATTACCCTCTTTTTACAGAAGCAATTTATGCTTTTGAAGTTCCAGAAGGCAGTAG ACTTGGTACTGTAGTGGGAACAGTTTGTGCCACGGATAAAGATGAGCCTGACACTATGCACACACGCCTC AAGTACAGCATCTTGGAGCAGACCCCACCATCACCTGGGCTTTTTTCTGTGCACCCTGACACAGGTGTCA TCACCACCGTCTCTCACTACATGGACAGAGAGGTCGTGGACAAGTACAAACTGATAATGAAAGTTCAGGA CATGAATGGCCAATTTTTTGGACTAATCAGTACATCAACTTGTATCATAACTGTGCAAGATTCCAATGAC AATGCACCCACATTCAGACAAAATACTTATGAAACTGCTGTGGAGGAAAATACATATAATGTTGAAATCT TGCGCATACCTGTAGATGATAAGGACATGATTAACACTGCCAACTGGAAGGCAAATTTCACCATCTTAAA AGGAAATGAAAATGGATGGTTCAAAATCACAACAGACCCAGTGACAAATGAAGGTGTTCTGTGTGTTGTG AAGCCACTGGATTATGAAGAAAATCGTCAAGTGACCCTAGAGATTGGTGTGAACAACGAAGCTCCATTCA TTAAAGATGTTGCAAACCGAATTCCCACCATGAACCGGGCTATGGTTACAGTACATGTGAAGGATCAGAA TGAGGGGCCAGAATGCAAGCCCCCAGAGCAATATGTGCGGATCAAAGAGAACTCAGCAGTTGGGTCTAAG ATCAATGGCTACAAGGCCTACGACCCTGAAACCAAGAATAGCAATGGCTTAAGGTACAAAAAATTGCAGG ATCCTAAAGACTGGGTCAGCATTGAAGAAGTTTCGGGGTTGCTTACCATATCAAAAACCCTAGACAGAGA AATAATGGCTCCAAGAAATGACATGTACAATATTACAGTCATGGCAATTGACCAAGAGGGTAAATCATGC ACAGGAACACTTGCAGTGAACATCGAGGATGTGAATGACAACGCACCAGAGATAATTCAAGATTATATTG TTATCTGTAAGCCAAAGATGGGGTACACGGACATTTCAGCTGTGGATCCTGATGAACCTATCCATGGCCC TCCCTTTCAGTTCAACTTGGCAAACACTTCTCCAGAAGTCAACAGAATCTGGACCCTCAACCAAGTTAAT GATACAGCTGCTCGCCTGTCCTATCAGAAAACTGCGGATGTTCAAATATACAACGTTCCTGTTACTGTAA AAGACAGAGCAGGCCAATCTGCAACCAAAATCTTGAGAGTTAACCTGTGTGATTGTACTCATCCCAGCCA ATGTCCGCTCAGATCAAGGAGTGCAGGAATTACCCTGGGAAAGTGGGCCATCCTTGCCATCTTGCTGGGC ATTGCTCTGCTATTTTCGGTACTGCTAACTTTGGTATGTGGAGTTGTTACTGCCCGAAAAGGAAAACACT TCCCTGAAGATTTAGCGCAGCAAAACTTAATTATATCAAATACCGAAGCACCTGGGGATGACAGAGTGTG CTCTGCCAATGGATTCACCACCCACACTGCCAACAACTCCAGCCAAGGCTTCTGTGGTACAATGGGGTCA GGAATGAGGAATGGTGGGCAGGAGACCATCGAGATGATGAAAGGACACCAGACCTTGGACTCCTGCCGTG TGGCCGGGCATCACCATACCCTGGACTCCGGCAGGGGAGGACACATGGATACTGACAACTGCAGATACAC ATACTCAGAGTGGCACAGTTTCACTCAACCCCGACTTGGTGAAGACTCCATTAGAGGACACACTGGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291809
Insert Size	2520 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291809.1, NP_001278738.1
RefSeq Size	5348 bp
RefSeq ORF	2520 bp
Locus ID	13507
UniProt ID	P55850
Gene Summary	This gene encodes a member of the cadherin family of proteins that mediates adhesion in desmosomes. Together with desmogleins, the encoded protein forms the transmembrane core of desmosomes, a multiprotein complex involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. Mice lacking the encoded protein exhibit a pre-implantation lethal phenotype. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature protein. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (2) contains an alternate exon in the 3' end compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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